Helicobacter heilmannii–associated primary gastric low-grade MALT lymphoma: Complete remission after curing the infection

Morgner, Andrea; Lehn, Norbert; Andersen, Leif Percival; Thiede, Christian; Bennedsen, Mads; Trebesius, Karlheinz; Neubauer, B.; Neubauer, Andreas; Stolte, Manfred; Bayerdörffer, Ekkehard

doi:10.1016/s0016-5085(00)70167-3

Cited by 265 publications

(203 citation statements)

References 29 publications

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“…Although H. heilmannii infection is rare in humans, it is considered to be of medical importance because of its association with gastritis, gastric ulcer, carcinoma, and mucosa-associated lymphoid tissue lymphoma (15)(16)(17)(18). In contrast to H. pylori infection, H. heilmannii more frequently colonizes the antral gastric mucosa.…”

Section: Introductionmentioning

confidence: 99%

<a name="home"></a>The role of IFN-gamma and IL-4 in gastric mucosa inflammation associated with Helicobacter heilmannii type 1 infection

Cinque

Rocha

Correa-Oliveira

et al. 2006

Braz J Med Biol Res

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Although Helicobacter heilmannii infection is less common than H. pylori infection in humans, it is considered to be of medical importance because of its association with gastritis, gastric ulcer, carcinoma, and mucosa-associated lymphoid tissue lymphoma of the stomach. However, there have been no studies evaluating the role of the Th cell response in H. heilmannii gastric infection. We evaluated the participation of pro-inflammatory and anti-inflammatory cytokines, IFN-γ and IL-4, in H. heilmannii gastric infection in genetically IFN-γ-or IL-4-deficient mice. The serum IFN-γ and IL-4 concentrations were determined by ELISA. The gastric polymorphonuclear infiltrate was higher (P = 0.007) in H. heilmannii-positive than in H. heilmanniinegative wild-type (WT) C57BL/6 mice, whereas no significant inflammation was demonstrable in the stomach of H. heilmannii-positive IFN-γ -/-C57BL/6 mice. The degree of gastric inflammatory cells, especially in oxyntic mucosa, was also higher (P = 0.007) in infected IL-4 -/-than in WT BALB/c mice. Serum IFN-γ levels were significantly higher in IL-4 -/-than in WT BALB/c mice, independently of H. heilmannii-positive or -negative status. Although no difference in serum IFN-γ levels was seen between H. heilmannii-positive (11.3 ± 3.07 pg/mL, mean ± SD) and -negative (11.07 ± 3.5 pg/mL) WT BALB/c mice, in the group of IL-4 -/-animals, the serum concentration of IFN-γ was significantly higher in the infected ones (38.16 ± 10.5 pg/ mL, P = 0.04). In contrast, serum IL-4 levels were significantly decreased in H. heilmannii-positive (N = 10) WT BALB/c animals compared to the negative (N = 10) animals. In conclusion, H. heilmannii infection induces a predominantly Th1 immune response, with IFN-γ playing a central role in gastric inflammation.

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Section: Introductionmentioning

confidence: 99%

<a name="home"></a>The role of IFN-gamma and IL-4 in gastric mucosa inflammation associated with Helicobacter heilmannii type 1 infection

Cinque

Rocha

Correa-Oliveira

et al. 2006

Braz J Med Biol Res

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“…"Candidatus Helicobacter heilmannii" has not been reliably cultured to date, but it can be maintained in mouse stomachs (2,23). In humans, "Candidatus Helicobacter heilmannii" infection has been associated with minimal gastritis, occasional gastroduodenal ulcers, and mucosa-associated lymphoid tissue (MALT)-type gastric B-cell lymphomas (MALT lymphomas) (15). Both H. pylori and "Candidatus Helicobacter heilmannii" infections have been reported to be related to these MALT lymphomas, while the relative significance of H. pylori and "Candidatus Helicobacter heilmannii" for MALT lymphoma formation is controversial.…”

mentioning

confidence: 99%

“…Both H. pylori and "Candidatus Helicobacter heilmannii" infections have been reported to be related to these MALT lymphomas, while the relative significance of H. pylori and "Candidatus Helicobacter heilmannii" for MALT lymphoma formation is controversial. Morgner et al showed that 1.47% of "Candidatus Helicobacter heilmannii"-infected patients and 0.66% of H. pylori-infected patients had primary gastric MALT lymphomas (15). On the other hand, Stolte et al reported a milder form of gastritis and less common MALT lymphomas in "Candidatus Helicobacter heilmannii" gastritis patients than in H. pylori-infected gastritis and duodenal ulcer patients, who are known to have less gastritis (26).…”

mentioning

confidence: 99%

“ Candidatus Helicobacter heilmannii” from a Cynomolgus Monkey Induces Gastric Mucosa-Associated Lymphoid Tissue Lymphomas in C57BL/6 Mice

et al. 2007

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Both Helicobacter pylori and "Candidatus Helicobacter heilmannii" infections are associated with peptic ulcers, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue (MALT) lymphomas. However, good animal models of H. pylori clinical diseases are rare. In this study, we aimed to establish an animal model of "Candidatus Helicobacter heilmannii" gastric MALT lymphoma. We used a urease-positive gastric mucosal and mucus homogenate from a cynomolgus monkey maintained in C57BL/6 mouse stomachs. The bacterium in the homogenate was identified as "Candidatus Helicobacter heilmannii" based on a DNA sequence analysis of the 16S rRNA and urease genes. Mucosal and mucus homogenates were used to inoculate C57BL/6 mice, which were then examined for 24 months. We observed a gradual increase in the surface area of protrusive lesions in almost all infected C57BL/6 mouse fundic stomachs 6 months after infection. Light microscopic observations revealed an accumulation of B lymphocytes along with destruction of glandular elements and the presence of lymphoepithelial lesions consistent with low-grade MALT lymphomas. Electron microscopic observation revealed numerous "Candidatus Helicobacter heilmannii" bacilli in the fundic glandular lumen, the intracellular canaliculi, and the cytoplasm of intact cells, as well as damaged parietal cells. In conclusion, "Candidatus Helicobacter heilmannii" induced gastric MALT lymphomas in almost 100% of infected C57BL/6 mice after a 6-month period associated with the destruction of parietal cells.

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“…Gastric biopsy specimens in 0.5 ml brain heart infusion broth were homogenized using tissue homogenizer (Kontes, Vineland, New Jersey) (Morgner et al, 2000). Then used for:…”

Section: Processing Of Gastric Biopsy Specimensmentioning

confidence: 99%

“…Direct smear was prepared by vigorously rubbing the suspended specimen on a sterile glass slide with a sterile loop. After air drying the smear was fixed by heating and stained with Gram's stain, (Morgner et al, 2000).…”

Section: Gram Stained Film From Culturementioning

confidence: 99%

Bacteriological Investigation of Helicobacter pylori Infections in Patients with Gastric and Duodenal Ulcer

Elshiekh¹,

Sultan²,

Zaky³

2017

Int.J.Curr.Microbiol.App.Sci

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Helicobacter heilmannii–associated primary gastric low-grade MALT lymphoma: Complete remission after curing the infection

Cited by 265 publications

References 29 publications

<a name="home"></a>The role of IFN-gamma and IL-4 in gastric mucosa inflammation associated with Helicobacter heilmannii type 1 infection

<a name="home"></a>The role of IFN-gamma and IL-4 in gastric mucosa inflammation associated with Helicobacter heilmannii type 1 infection

“ Candidatus Helicobacter heilmannii” from a Cynomolgus Monkey Induces Gastric Mucosa-Associated Lymphoid Tissue Lymphomas in C57BL/6 Mice

Bacteriological Investigation of Helicobacter pylori Infections in Patients with Gastric and Duodenal Ulcer

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