Heat sensitivity of a SARS‐associated coronavirus introduced into plasma products

Abstract

If by rare chance SARS-CoV contaminates source plasma, there should be no or only minor risk of this virus infection, due to sufficient inactivation by the 60 degrees C 10 h liquid heating step, although we must pay attention to the composition used for blood product preparation.

Evaluation of inactivation methods for severe acute respiratory syndrome coronavirus in noncellular blood products

“…We found that 90 minutes at either 56 or 65°C was necessary to inactivate the virus. 6 Yunoki and associates 21 examined the sensitivity of SARS-CoV to 60°C heat and found that the different protein compositions of the samples tested affected the time necessary, yet all samples were inactivated to the limit of detection by 1 hour. Additionally, Rabenau and coworkers 22 tested heat inactivation of SARS-CoV at 56°C for 30 minutes and determined that the presence of 20 percent fetal calf serum protected the virus.…”

Coronavirus Disease 2019: Coronaviruses and Blood Safety

“…In-house studies of methods to inactivate coronaviruses in plasma and platelet concentrates focused mainly on heat and solvent/detergent (S/D) treatment. Usually, 60°C for 15-30 minutes is enough for reduction of SARS-CoV from plasma without cells [49], and inactivation could be achieved by 60°C for 10 hours for plasma products [52]. In the other study, heating at 56°C for 25 minutes could reduce more than 4 log 10 TCID 50 /mL of MERS virus [53].…”

“…The infectious titer of virus did not show any significant reduction after 25 cycles of thawing and freezing [44]. After the outbreak of SARS and MERS, a few studies investigated pathogen inactivation/reduction technologies (PRTs) based on in-house or commercial methods with the aim to decrease or completely eradicate the potential risk of transmission of coronaviruses via blood products or blood derivatives [45][46][47][48][49][50][51][52][53][54]. These studies are summarized in Table 1.…”

Temperature Decreases Spread Parameters of the New Covid-19 Case Dynamics

“…The changes in weather alone would not necessarily decrease numbers of confirmed cases during the whole epidemic, but warm and humid weather could make SARS-CoV-2 less transmissible and less stable, provoking a break in the chain of transmission, and hence diminishing the contagious force of the disease [5]. Ancient [1,4,5] studies, as well as characteristics of the covid-19 disease compared to past corona infections like the SARS one [3,[6][7][8], suggest that the spread of covid-19 could diminish in warm weather, particularly at the start of the epidemic, and may have a low temperature threshold under which it could spread fastest. These seasonal changes could occur in exactly the same way as for other pathogens, like the common cold or influenza [9][10][11][12].…”