Heat acclimation causes some alterations in serum enzymes activities in streptozotocin-diabetic rats

Domazetovska, Sashka; Dinevska-Ќovkarovska, Suzana; Miova, Biljana; Bogdanova, Maria; Mitev, Slavco

doi:10.1016/j.jtherbio.2011.03.007

Cited by 1 publication

(1 citation statement)

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“…Rats were randomly distributed into HA or C group. HA group was maintained in chamber temperature of 35 ± 1°C with a relative humidity of 55 ± 5% 2 h daily for 30 days to induce homeostasis and their body weight Tre changes were monitored (Dervisevik et al, 2011; Domazetovska et al, 2011; Alexander-Shani et al, 2017). Tre was measured under the arousal condition of food and water ad libitum .…”

Section: Methodsmentioning

confidence: 99%

Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated Rats

Fan

Wang

et al. 2019

Front. Physiol.

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Circular RNAs (circRNAs) have vital roles in great variety of biological processes. However, expression levels and functions of circRNAs related to heat acclimation (HA) are poorly understood. This study is the first time an in-depth circRNA expression profiling were used to investigate circRNA–miRNA interactions in HA rats in order to further comprehend the mechanisms underlying HA. CircRNA expression profile was performed in rats’ hypothalamus of HA and control group with microarray assays and their functions were predicted by using Bioinformatics analysis. Differential circRNAs and their regulated downstream miRNAs and mRNAs were quantitatively validated by means of quantitative polymerase chain reaction in real-time (RT-qPCR). Enzyme-linked immunosorbent assay (ELISA) was then applied to predict the expression of proteins. In total, 53 circRNAs were expressed distinctively between the HA and Control; up- and down-regulation of circRNAs were 28 and 25, respectively, in HA (fold change > 1.5, P < 0.05). Three circRNAs and two miRNAs and three predicted mRNAs were obviously regulated after validated by RT-qPCR in HA rats. Two proteins expression were proportional to their mRNA changes. Further analysis demonstrates that circRNAs closest to HA can be categorized into three signal pathways: including rno_circRNA_014301-vs-rno-miR-3575-vs- Hif-1 α, rno_circRNA_014301-vs-rno-miR-3575-vs- Lppr4 , and rno_circRNA_010393-vs-rno-miR-20b-3p-vs- Mfap4 in hypoxia response pathways, substance/energy metabolism, and inflammatory response pathways. Our findings implicate that many circRNAs regulate expressions of genes that interact with each other to exert their functions during HA.

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Section: Methodsmentioning

confidence: 99%