Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated Rats

Fan, Lijun; An, Gaihong; Wang, Shang; Chen, Xuewei; Liu, Ying; Liu, Zhifeng; Ma, Qiang; Wang, Jing

doi:10.3389/fphys.2019.01112

Cited by 12 publications

(12 citation statements)

References 44 publications

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“…Furthermore, the metabolic pathway enrichment analysis identified a change in different metabolic pathways, such as biosynthesis of unsaturated fatty acids, linoleic acid metabolism, GnRH signaling pathway, mTOR signaling pathway, phenylalanine metabolism, and arginine biosynthesis. The previous proteomic and gene sequencing results also showed that HE might affect immune-related signaling pathways and carbohydrate/lipoprotein metabolism–related signaling pathways ( Wang et al, 2016 ; Fan et al, 2019 ). The results suggested that the alterations in the levels of different metabolites might lead to ECD during HE and affect the health of the endocrine, immune, and reproductive systems of rats through the lipid metabolism pathway, amino acid metabolism pathway, mTOR signaling pathway, and GnRH signaling pathway.…”

Section: Discussionmentioning

confidence: 88%

Integrative Analysis of Vaginal Microorganisms and Serum Metabolomics in Rats With Estrous Cycle Disorder Induced by Long-Term Heat Exposure Based on 16S rDNA Gene Sequencing and LC/MS-Based Metabolomics

Fan

Chen

et al. 2021

Front. Cell. Infect. Microbiol.

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Long term heat exposure (HE) leads to estrous cycle disorder (ECD) in female rats and damages reproductive function. However, the regulation mechanism of vaginal microorganisms and serum metabolomics remains unclear. This study aimed to explore the effects of microbes on the vaginal secretions of rats with ECD and describe the serum metabolomics characteristics and their relationship with vaginal microorganisms. The alterations in the serum levels of neurotransmitters were used to verify the possible regulatory pathways. The relative abundance, composition, and colony interaction network of microorganisms in the vaginal secretions of rats with ECD changed significantly. The metabolomics analysis identified 22 potential biomarkers in the serum including lipid metabolism, amino acid metabolism, and mammalian target of rapamycin and gonadotropin-releasing hormone (GnRH) signaling pathways. Further, 52 pairs of vaginal microbiota–serum metabolites correlations (21 positive and 31 negative) were determined. The abundance of Gardnerella correlated positively with the metabolite L-arginine concentration and negatively with the oleic acid concentration. Further, a negative correlation was found between the abundance of Pseudomonas and the L-arginine concentration and between the metabolite benzoic acid concentration and the abundance of Adlercreutzia. These four bacteria–metabolite pairs had a direct or indirect relationship with the estrous cycle and reproduction. The glutamine, glutamate, and dopamine levels were significantly uncontrolled. The former two were closely related to GnRH signaling pathways involved in the development and regulation of HE-induced ECD in rats. Serum neurotransmitters partly reflected the regulatory effect of vaginal microorganisms on the host of HE-induced ECD, and glutamatergic neurotransmitters might be closely related to the alteration in vaginal microorganisms. These findings might help comprehend the mechanism of HE-induced ECD and propose a new intervention based on vaginal microorganisms.

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Section: Discussionmentioning

confidence: 88%

Integrative Analysis of Vaginal Microorganisms and Serum Metabolomics in Rats With Estrous Cycle Disorder Induced by Long-Term Heat Exposure Based on 16S rDNA Gene Sequencing and LC/MS-Based Metabolomics

Fan

Chen

et al. 2021

Front. Cell. Infect. Microbiol.

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“…Typically, rat T core > 42 ± 0.5°C is defined as a thermal injury (heat stock) marker [ 22 , 23 ]. In the current study [ 7 , 24 ], the temperature was limited to 38.0 ± 0.5°C, >36.0°C, and< 41.0°C, which is “compensable heat stress” [ 25 ]. The T core was invariable between 38.0 and 39.5°C, without any obvious abnormal behavior or death in the heat stress group rats.…”

Section: Discussionmentioning

confidence: 99%

“…The body weight and rectal temperature of the animals were measured before and after 2 h heat exposure every 10 days. Digital balance (Jiangsu, Tong Jun) and thermal probe (1529 thermometer, Fluke Corporation) were used to detect the body weight and rectal temperature, respectively [7].…”

Section: Body Weight and Rectal Temperaturementioning

confidence: 99%

Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress

An¹,

Chen²,

Li³

et al. 2020

BioMed Research International

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High-temperature exposure is detrimental to women’s reproductive health; however, the impact caused by long-term high temperature is not comprehensive, and a stable model of estrous cycle disorder induced by a high temperature is yet lacking. Herein, we aimed to establish a stable and effective model of estrous cycle disorder in female rats induced by long-term heat stress to study its physiological and pathological characteristics and explore the underlying mechanism. In the present study, female Sprague-Dawley rats with normal estrous cycles were exposed to the temperature of 38±0.5°C, relative humidity (RH) of 55±5% (2 h/d, 1 time/d) hot cabin at more than 90 days. Consequently, after long-term heat stress, no difference was detected in body weight and rectal temperature, but the estrus cycle was prolonged, the uterine organ index was increased, pathological changes occurred, the increase latitude of stress hormones heat shock protein 70 (Hsp70) and corticosterone (CORT) decreased, estradiol (E2) and luteinizing hormone (LH) levels decreased, follicle stimulating hormone (FSH) and prolactin (Prl) levels increased, gonadotropin-releasing hormone (GnRH) and thyroid hormone (T4) showed no difference, and insulin (INS) decreased significantly. Moreover, the mRNA expression of the sex hormone receptor in the uterus and ovary was altered. Therefore, the estrous cycle disorder in female rats can be induced by regular heat stress for 90 days, which can be considered the pioneer method. Subsequently, prominent physiological and pathological characteristics and disruption in the hypothalamic-pituitary-gonadal (HPG) axis were noted.

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“…It is a stress factor that special operating populations have to guard against in high-temperature environments (Kovats and Hajat, 2008). During heat stress, the Frontiers in Physiology frontiersin.org normal thermal homeostasis system of the body may be disrupted, leading to the disruption of digestive somatic functions, impaired blood circulation, and disruption of neuroendocrine functions, which together pose a severe threat to the development of reproductive health concerns in women working under high-temperature environments (Wang et al, 2016;Fan et al, 2019). Menstrual disorders not only affect female reproduction but may also lead to infertility.…”

Section: Menstrual Disorders Caused By High Temperature Environmentmentioning

confidence: 99%

Characteristics of menstrual cycle disorder and saliva metabolomics of young women in a high-temperature environment

Wei

An²,

Fan³

et al. 2023

Front. Physiol.

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Objective: Menstrual disorders induced by high-temperature environments, can seriously damage women’s reproductive health and workability. The regulation mechanism underlying it is not yet to be elucidated. Saliva is an information-rich biological fluid that can reflect systemic diseases. Here, we investigated the characteristics of menstrual cycle disorders and saliva metabolomics to provide a deeper insight of the regulation mechanism of young women in high-temperature environments.Methods: Women from high and normal temperature areas of China were selected and divided into two groups—high-temperature (H group) and control (C group). A questionnaire survey was conducted in summer (July) to investigate the incidence rate of menstrual disorders, characteristics of the disorders, and factors influencing the risk of these disorders in different regions. Metabolomics was applied to analyze the characteristics of the salivary metabolites and neurotransmitters in the two groups of women with menstrual disorders.Results: The incidence rate of menstrual disorders was significantly higher in the H group than that in the C group (p < 0.05). High-temperature environment, stress, and sleep quality were identified as critical factors associated with menstrual disorders. Non-targeted saliva metabolomics identified 64 significantly different metabolites between two groups, which mainly enriched in metabolic pathways such as carbohydrate metabolism, membrane transport, digestive system, and nucleotide metabolism (p < 0.05). N-acetylneuraminic acid, MYO, and tyramine may be candidate markers for early diagnosis of menstrual disorders in high temperature environments. Metabolites may be involving in the acute-phase response during an inflammatory process, to affecting the reproductive system by influencing the HPA axis loop. Regulations about oocyte membrane production and the luteal functions would be exerted in menstrual disorders. Targeted metabolomics of neurotransmitters revealed increased expression of histamine (HA) and glutamine and decreased expression of 5-hydroxyindole acetic acid (5-HIAA) (p < 0.05).Conclusion: Menstrual disorder characteristics induced by high temperature environments were specific. Anxiety, sleep quality and temperature feeling were the key factors to the menstrual disorder. endocrine regulation mechanism and inflammatory reactions might contribute to the development of menstrual disorders through influencing the formation of the follicular cell membrane.

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Circular RNA Expression Profiling and Selection of Key Circular RNAs in the Hypothalamus of Heat-Acclimated Rats

Cited by 12 publications

References 44 publications

Integrative Analysis of Vaginal Microorganisms and Serum Metabolomics in Rats With Estrous Cycle Disorder Induced by Long-Term Heat Exposure Based on 16S rDNA Gene Sequencing and LC/MS-Based Metabolomics

Integrative Analysis of Vaginal Microorganisms and Serum Metabolomics in Rats With Estrous Cycle Disorder Induced by Long-Term Heat Exposure Based on 16S rDNA Gene Sequencing and LC/MS-Based Metabolomics

Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress

Characteristics of menstrual cycle disorder and saliva metabolomics of young women in a high-temperature environment

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