Healing of tibial and calvarial bone defect using Runx-2-transfected adipose stem cells

Enhanced Bone Repair by Guided Osteoblast Recruitment Using Topographically Defined Implant

“…After shaving the scalp hairs, a longitudinal incision was made in the midline of the cranium from the nasal bone to the posterior nuchal line, and the periosteum was elevated to expose the surface of the parietal bones. Using a surgical trephine bur (Ace Surgical Supply Co., Brockton, MA) and a low-speed micromotor, two circular and transosseous defects with a diameter of 4 mm, the critical size of mouse calvarial defect, 22 were produced in the skull. The drilling sites were irrigated with saline and the bleeding points were electrocauterized.…”

Improved vascularisation but inefficient in vivo bone regeneration of adipose stem cells and poly-3-hydroxybutyrate-co-3-hydroxyvalerate scaffolds in xeno-free conditions

“…Both PHB-HV and PHB-HV + hASCs constructs modulated RUNX2, ALPL, BGLAP and COL1A1 expression; hASCs addition further augmented their expression. The expression of these bone markers mRNA before osteoblast mineralisation initiation suggests that the proteins may be involved in the preparation of the extracellular matrix for the ordered deposition of minerals which is necessary for the progressive formation of new bone tissue [29]. The higher BGLAP expression 12 weeks after implantation is in accordance with its expression only postproliferatively with the onset of nodule formation.…”

“…There are some differences between our studies and others with better outcome with hASCs for bone regeneration. Firstly, we did not follow any pre-differentiation protocols [41,42] and hASCs genetically modified [29,43,44] in vitro before grafting as some studies did it. The athymic nude mouse model of nonhealing critical-sized calvarial defect was chosen instead of using an immunocompetent animal model, because it is commonly used to evaluate bone regeneration [45,46] and it lacks T cells being well established for xenogeneic transplants, which would have contributed for long term persistence of hASCs in an in vivo graft and promoted earlier neovascularisation.…”

Epidermal Growth Factor (EGF)-Like Repeats and Discoidin I-Like Domains 3 (EDIL3): A Potential New Therapeutic Tool for the Treatment of Keloid Scars

“…A viral vector can ensure stable gene expression and high transfection efficiency compared with nonviral vectors. On the other hand, viral vectors have a risk of toxicity to the recipient and also can induce an inflammatory response [38,40,41]. Microporation, which uses a nonviral vector, protected the keloidspecific cellular characteristics and established highly efficient transformation protocol that ensured optimal conditions for KFs.…”