Harnessing the action mechanisms of microbial endophytes for enhancing plant performance and stress tolerance: current understanding and future perspectives

Kaur, Gagandeep; Patel, Arvind; Dwibedi, Vagish; Rath, Santosh Kumar

doi:10.1007/s00203-023-03643-4

Cited by 5 publications

(5 citation statements)

References 169 publications

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“…In the future, their nitrogen fixation and nutrient solubilization activities may be further tested after being applied to the field. Additional challenges associated with developing these endophytic bacteria into commercial biofertilizer products include successful product refinement, the passing of toxicology analysis/evaluation, and prototype formulation [90].…”

Section: Discussionmentioning

confidence: 99%

Species Diversity, Nitrogen Fixation, and Nutrient Solubilization Activities of Endophytic Bacteria in Pea Embryos

Hao,

Zhang,

Qiu

et al. 2024

Applied Sciences

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Endophytic bacteria, especially those that participate in nitrogen fixation, play critical roles in supplying essential nutrients for legume plant growth. Despite that there have been numerous investigations targeting bacterial microbiomes in legume roots and nodules, little is known about embryonic bacteria that facilitate plant nutrient utilization after seed germination. Here, we collected and investigated endophytic bacterial microbiome in edible pea (Pisum sativum) embryos using five representative cultivars and a pea sprout (shoot of pea [SHP]) control. Twenty-six nitrogen-fixing bacteria (NFB) were isolated from pea embryos, with three strains found in fresh grain pea (FGP) and snow pea (SP) exhibiting the strongest nitrogenase activity of above 85 nmol C2H4/mL/h. Some NFB isolates are also potassium-solubilizing bacteria (KSB) or phosphorus-solubilizing bacteria (PSB) utilizing inorganic and/or organic phosphorus. All 26 NFB showed variable levels (0.41 to 7.10 μg/mL) of indole-3-acetic acid (IAA) secretion. The nutrient-solubilizing NFB identified in our research are potential targets for biofertilizer development. They could be useful in converting nitrogen, potassium, and/or phosphorus into usable forms for the plants. At the microbiome level, high-throughput 16S ribosomal RNA (rRNA) sequencing of 40 bacterial collections from pea embryos generated 4234 operational taxonomic units (OTUs) using 97% identity as the threshold for clustering high-quality effective reads (valid tags). Analysis of OTU annotation results revealed similar species community structures, abundance, and diversity in most samples. Our embryo-derived endophytic bacterial pool provides a microbiome platform for seed dormancy and germination research of edible peas, as well as for digging new biofertilizer resources in general.

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Section: Discussionmentioning

confidence: 99%

Species Diversity, Nitrogen Fixation, and Nutrient Solubilization Activities of Endophytic Bacteria in Pea Embryos

Hao,

Zhang,

Qiu

et al. 2024

Applied Sciences

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“…Abiotic factors like drought, salt, heat, freezing, heavy metal toxicity, hypoxia and anoxia, waterlogging, and nutritional imbalance are the most severe constraints leading to a drastic decline in crop production (about 51-82%), which hampers global food and nutritional security (Khare and Arora, 2015;Cooke and Leishman, 2016;Yadav et al, 2020;Del Buono, 2021;Raza et al, 2022;Kaur et al, 2023). These stressors have become more common over the past several decades, mainly as a result of the aberrant weather fluctuations triggered by climate change.…”

Section: Endophytic Microbes For Abiotic Stress Tolerance In Host Med...mentioning

confidence: 99%

Deciphering the mechanisms, hormonal signaling, and potential applications of endophytic microbes to mediate stress tolerance in medicinal plants

Pandey,

Tripathi,

Dwivedi

et al. 2023

Front. Plant Sci.

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The global healthcare market in the post-pandemic era emphasizes a constant pursuit of therapeutic, adaptogenic, and immune booster drugs. Medicinal plants are the only natural resource to meet this by supplying an array of bioactive secondary metabolites in an economic, greener and sustainable manner. Driven by the thrust in demand for natural immunity imparting nutraceutical and life-saving plant-derived drugs, the acreage for commercial cultivation of medicinal plants has dramatically increased in recent years. Limited resources of land and water, low productivity, poor soil fertility coupled with climate change, and biotic (bacteria, fungi, insects, viruses, nematodes) and abiotic (temperature, drought, salinity, waterlogging, and metal toxicity) stress necessitate medicinal plant productivity enhancement through sustainable strategies. Plants evolved intricate physiological (membrane integrity, organelle structural changes, osmotic adjustments, cell and tissue survival, reclamation, increased root-shoot ratio, antibiosis, hypersensitivity, etc.), biochemical (phytohormones synthesis, proline, protein levels, antioxidant enzymes accumulation, ion exclusion, generation of heat-shock proteins, synthesis of allelochemicals. etc.), and cellular (sensing of stress signals, signaling pathways, modulating expression of stress-responsive genes and proteins, etc.) mechanisms to combat stresses. Endophytes, colonizing in different plant tissues, synthesize novel bioactive compounds that medicinal plants can harness to mitigate environmental cues, thus making the agroecosystems self-sufficient toward green and sustainable approaches. Medicinal plants with a host set of metabolites and endophytes with another set of secondary metabolites interact in a highly complex manner involving adaptive mechanisms, including appropriate cellular responses triggered by stimuli received from the sensors situated on the cytoplasm and transmitting signals to the transcriptional machinery in the nucleus to withstand a stressful environment effectively. Signaling pathways serve as a crucial nexus for sensing stress and establishing plants’ proper molecular and cellular responses. However, the underlying mechanisms and critical signaling pathways triggered by endophytic microbes are meager. This review comprehends the diversity of endophytes in medicinal plants and endophyte-mediated plant-microbe interactions for biotic and abiotic stress tolerance in medicinal plants by understanding complex adaptive physiological mechanisms and signaling cascades involving defined molecular and cellular responses. Leveraging this knowledge, researchers can design specific microbial formulations that optimize plant health, increase nutrient uptake, boost crop yields, and support a resilient, sustainable agricultural system.

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“…One of the important strategies for improving food security and maintaining soil fertility is breeding bean cultivars resistant to drought conditions [11,12] and capable of a high level of nodule-forming activity [13,14]. The ability of plants to tolerate unfavorable environmental conditions can be enhanced by inoculation with highly effective strains of endophytic bacteria (EB) [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Despite the fact that EB preparations do not exhibit strict species specificity [18], the effect of inoculation depends on the genotype of the host plant [17,33,34,38,39,43] and doses of introduced bacteria [44]. For effective plant cultivation, it is important to address the possibilities of controlling plant productivity using the potential of EB and the limiting factors constraining this process [15][16][17][18]. According to recent studies, it is necessary to select the strain-dose combination individually for each plant genotype [17,18,44].…”

Section: Introductionmentioning

confidence: 99%

“…For effective plant cultivation, it is important to address the possibilities of controlling plant productivity using the potential of EB and the limiting factors constraining this process [15][16][17][18]. According to recent studies, it is necessary to select the strain-dose combination individually for each plant genotype [17,18,44]. In order to conduct such screening, it is important to determine the phenotypic traits of the inoculated plants in the early stages of ontogenesis that correlate with the field effectiveness of bacterial preparations.…”

Section: Introductionmentioning

confidence: 99%

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Predicting Field Effectiveness of Endophytic Bacillus subtilis Inoculants for Common Bean Using Morphometric and Biochemical Markers

Markova,

Garipova,

Chistoedova

et al. 2024

Plants

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According to four field experiments, after the inoculation of Phaseolus vulgaris L. cultivar Ufimskaya with the commercial strain Bacillus subtilis 26D and the promising strain B. subtilis 10–4, it was found that inoculation with B. subtilis 10–4 improved seed productivity (SP) by 31–41% per plant, but only in dry years. In contrast, all 4 years of inoculation with B. subtilis 26D were ineffective or neutral. It was intended to determine the growing and biochemical characteristics of inoculated 7-day-old plants, which correlate with the field SP of bacterial preparations. The SP of inoculated plants (average of 4 years) correlated with root length (0.83), MDA content (−0.98), and catalase (CAT) activity in roots (−0.96) of week-old seedlings. High correlation coefficients between the H2O2 content in the roots and SP (0.89 and 0.77), as well as between the H2O2 content in shoots and SP (0.98 and 0.56), were observed only in two dry years, when the influence of bacteria was detected. These physiological indicators were identified as potential markers for predicting the effectiveness of the endophytic symbiosis between bean plants and B. subtilis strains. The findings may be used to develop effective microbial-based, eco-friendly technologies for bean production.

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Harnessing the action mechanisms of microbial endophytes for enhancing plant performance and stress tolerance: current understanding and future perspectives

Cited by 5 publications

References 169 publications

Species Diversity, Nitrogen Fixation, and Nutrient Solubilization Activities of Endophytic Bacteria in Pea Embryos

Species Diversity, Nitrogen Fixation, and Nutrient Solubilization Activities of Endophytic Bacteria in Pea Embryos

Deciphering the mechanisms, hormonal signaling, and potential applications of endophytic microbes to mediate stress tolerance in medicinal plants

Predicting Field Effectiveness of Endophytic Bacillus subtilis Inoculants for Common Bean Using Morphometric and Biochemical Markers

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