Hard X-ray microtomography of Zebrafish larvae

Osterwalder, Melissa; Bolten, Jan Stephan; Rodgers, Griffin; Humbel, Mattia; Schulz, Georg; Tanner, Christine; Huwyler, Jörg; Müller, Bert

doi:10.1117/12.2593119

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…This study promises a broader understanding of anatomy and corresponding physiology and pathophysiology. Previously, we showed that more than 50 % of anatomical features identified by synchrotron radiation-based micro computed tomography (SRµCT) can also be identified with standard laboratory-based tomography systems [37]. We can, therefore, expect that the cutting-edge laboratory-based systems provide images comparable to the tomography setups at synchrotron radiation facilities.…”

Section: Zebrafish Embryoa Versatile Biomedical Research Modelmentioning

confidence: 99%

Laboratory-based phase and absorption tomography for micro-imaging of annual layers in human tooth cementum, paraffin-embedded nerve and zebrafish embryo

Migga

Schulz

Rodgers³

et al. 2021

Developments in X-Ray Tomography XIII

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Synchrotron radiation-based tomography yields the micro-anatomical features in human and animal tissues without physical slicing. Recent advances in instrumentation have made laboratory-based phase tomography feasible. We compared the performance of three cutting-edge laboratory systems benchmarked by synchrotron radiation-based tomography for three specimens. As an additional criterion, the user-friendliness of the three microtomography systems is considered. The three tomography systems-SkyScan 2214 (Bruker-microCT, Kontich, Belgium), Xradia 620 Versa (Zeiss, Oberkochen, Germany) and the prototype of Exciscope (Stockholm, Sweden)-were given 36 hours to measure three medically relevant specimens-archaeological human tooth, porcine nerve and zebrafish embryo. The obtained datasets were registered to the benchmark synchrotron radiation-based tomography from the same specimens and to the SkyScan 1275 and phoenix nanotom m ® laboratory systems to characterize the development during the last decade. The next-generation laboratory-based microtomography almost reached the quality of synchrotron-radiation facilities with respect to spatial and density resolution, as indicated by the visualization of the medically relevant micro-anatomical features. The Exciscope prototype and the SkyScan 2214 demonstrated the complementarity of phase information by imaging the eyes of the zebrafish embryo. The Xradia 620 Versa enabled the three-dimensional visualization of the 2 to 3 µm-thin annual layers in tooth cementum. The data of the Exciscope prototype with the high photon flux from the liquid metal source from Excillum (Kista, Sweden) showed the spiral nature of the myelin sheaths in the porcine nerve. The SkyScan 2214, the most effortable among the three next-generation systems evaluated, was well suited to visualize the wealth of anatomical features in the zebrafish embryo.

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Section: Zebrafish Embryoa Versatile Biomedical Research Modelmentioning

confidence: 99%

Laboratory-based phase and absorption tomography for micro-imaging of annual layers in human tooth cementum, paraffin-embedded nerve and zebrafish embryo

Migga

Schulz

Rodgers³

et al. 2021

Developments in X-Ray Tomography XIII

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“…The use of three-dimensional visualization techniques is crucial in this regard [9][10][11][12][13]. Among these, the X-Ray microtomography (microCT) technique is gaining popularity for researching both healthy embryogenesis and developmental abnormalities [14][15][16]. This is a non-destructive method of visualizing the internal structure of biological objects.…”

Section: Introductionmentioning

confidence: 99%

High-Performance Microcomputing Tomography of Chick Embryo in the Early Stages of Embryogenesis

Rzhepakovsky,

Piskov,

Avanesyan

et al. 2023

Applied Sciences

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X-ray contrast techniques were tested on the chick embryos in early periods of embryogenesis. For contrast stain, reagents with radiopacity in various concentrations were used: silver proteinate, eosin, Lugol’s solution (I2KI), phosphomolybdic acid and phosphotungstic acid under heating at 25 °C and 40 °C and exposure for 24 and 48 h. The use of silver proteinate, eosin and I2KI in various concentrations in the contrast of the chick embryo in the early period of embryogenesis did not make it possible to obtain microtomographic results that provide reliable microstructural analysis. The most optimal and effective method of embryo staining at the HH22–HH34 embryonic stages reliably determined the staining of 1% phosphotungstic acid at 40 °C heating and exposure for 24 h. Taking into account the size of the chick embryos and their structures at the HH22–HH34 embryonic stages, the features of the development, location of organs, and the minimum permissible parameters of microtomography for obtaining high-quality and reliable results were determined by the isometric spatial resolution of 8.87 μm, X-ray voltage 50 kV, X-ray current 500 μA, and the use of filters started from Al 0.5 mm. Microtomographic results were obtained, characterized by the appearance of the chick embryo at the HH22–HH34 embryonic stages, and they visualized the locations and structures of the chick embryo organs and provided calculation of their volume and X-ray density. The results of the work open up significant prospects for using the chick embryo at the early embryonic period of embryogenesis as an alternative model for screening teratogenicity.

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“…Previously, we showed that more than 50% of anatomical features identified by synchrotron radiation-based microcomputed tomography (

) can also be identified with standard laboratory-based tomography systems. 27 We can, therefore, expect that the cutting-edge laboratory-based systems will provide images comparable to the tomography setups at synchrotron radiation facilities. Such a level of success implies the possibility to easily perform large experimental series of high-resolution imaging fundamental in zebrafish larva-based research activities.…”

Section: Introductionmentioning

confidence: 99%

Comparative hard x-ray tomography for virtual histology of zebrafish larva, human tooth cementum, and porcine nerve

et al. 2022

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Purpose: Synchrotron radiation-based tomography yields microanatomical features in human and animal tissues without physical slicing. Recent advances in instrumentation have made laboratory-based phase tomography feasible. We compared the performance of three cutting-edge laboratory systems benchmarked by synchrotron radiation-based tomography for three specimens. As an additional criterion, the user-friendliness of the three microtomography systems was considered.Approach: The three tomography systems-SkyScan 2214 (Bruker-microCT, Kontich, Belgium), Exciscope prototype (Stockholm, Sweden), and Xradia 620 Versa (Zeiss, Oberkochen, Germany)were given 36 h to measure three medically relevant specimens, namely, zebrafish larva, archaeological human tooth, and porcine nerve. The obtained datasets were registered to the benchmark synchrotron radiation-based tomography from the same specimens and selected ones to the SkyScan 1275 and phoenix nanotom m ® laboratory systems to characterize development over the last decade.Results: Next-generation laboratory-based microtomography almost reached the quality achieved by synchrotron-radiation facilities with respect to spatial and density resolution, as indicated by the visualization of the medically relevant microanatomical features. The SkyScan 2214 system and the Exciscope prototype demonstrated the complementarity of phase information by imaging the eyes of the zebrafish larva. The 3-μm thin annual layers in the tooth cementum were identified using Xradia 620 Versa.

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Hard X-ray microtomography of Zebrafish larvae

Cited by 4 publications

References 26 publications

Laboratory-based phase and absorption tomography for micro-imaging of annual layers in human tooth cementum, paraffin-embedded nerve and zebrafish embryo

Laboratory-based phase and absorption tomography for micro-imaging of annual layers in human tooth cementum, paraffin-embedded nerve and zebrafish embryo

High-Performance Microcomputing Tomography of Chick Embryo in the Early Stages of Embryogenesis

Comparative hard x-ray tomography for virtual histology of zebrafish larva, human tooth cementum, and porcine nerve

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