Comparative hard x-ray tomography for virtual histology of zebrafish larva, human tooth cementum, and porcine nerve

Abstract

Purpose: Synchrotron radiation-based tomography yields microanatomical features in human and animal tissues without physical slicing. Recent advances in instrumentation have made laboratory-based phase tomography feasible. We compared the performance of three cutting-edge laboratory systems benchmarked by synchrotron radiation-based tomography for three specimens. As an additional criterion, the user-friendliness of the three microtomography systems was considered.Approach: The three tomography systems-SkyScan 2214 (Bruker-microCT, Kontich, Belgium), Exciscope prototype (Stockholm, Sweden), and Xradia 620 Versa (Zeiss, Oberkochen, Germany)were given 36 h to measure three medically relevant specimens, namely, zebrafish larva, archaeological human tooth, and porcine nerve. The obtained datasets were registered to the benchmark synchrotron radiation-based tomography from the same specimens and selected ones to the SkyScan 1275 and phoenix nanotom m ® laboratory systems to characterize development over the last decade.Results: Next-generation laboratory-based microtomography almost reached the quality achieved by synchrotron-radiation facilities with respect to spatial and density resolution, as indicated by the visualization of the medically relevant microanatomical features. The SkyScan 2214 system and the Exciscope prototype demonstrated the complementarity of phase information by imaging the eyes of the zebrafish larva. The 3-μm thin annual layers in the tooth cementum were identified using Xradia 620 Versa.

Zebrafish (Danio rerio) larvae as a predictive model to study gentamicin-induced structural alterations of the kidney

“…However, this did not offer additional advantages as images were of similar quality. It is tempting to speculate that recent laboratory-based tomography instruments providing phase-contrast capabilities, liquid metal X-ray source technology, and X-ray detector optics may provide a spatial and density resolution comparable to the present study [ 25 ]. This will provide opportunities for a broader applicability of the presented tomography-based approach, although at the expense of longer acquisition times.…”

Synchrotron X-ray Studies of the Structural and Functional Hierarchies in Mineralised Human Dental Enamel: A State-of-the-Art Review

“…The data were extracted from Endnote software in ‘.txt’ format and manually edited to arrange the studies by techniques. In total, 31 terms were considered based on these studies [ 11 , 31 , 39 , 56 , 57 , 77 , 78 , 89 , 90 , 121 , 169 , 170 , 171 , 172 , 173 , 175 , 193 , 195 , 247 , 266 , 286 , 294 , 299 , 303 , 304 , 305 , 306 , 307 , 308 , 309 , 310 , 311 , 312 , 313 , 314 , 315 , 316 , 317 , 318 , 319 , 320 , 321 , 322 , 323 , 324 , 325 , 326 , 327 , 328 , 329 , 330 , ...…”

“…The data were extracted from Endnote software (Clarivate) in ‘.txt’ format and manually edited to match the reading format and correlation of words; for example, British spelling was applied when translating non-English titles and acronyms were generally written in full length. The minimum number of occurrences of a term was set to four, and in total 140 terms were used based on these studies [ 11 , 31 , 39 , 56 , 57 , 77 , 78 , 89 , 90 , 121 , 169 , 170 , 171 , 172 , 173 , 175 , 193 , 195 , 247 , 266 , 286 , 294 , 299 , 303 , 304 , 305 , 306 , 307 , 308 , 309 , 310 , 311 , 312 , 313 , 314 , 315 , 316 , 317 , 318 , 319 , 320 , 321 , 322 , 323 , 324 , 325 , 326 , 327 ,…”

Three-dimensional imaging and analysis of annual layers in tree trunk and tooth cementum

“…The successful imaging of the layered structures in the tooth cementum of the African buffalo could be regarded as a milestone towards the identification of previous hunting seasons. As the periodicity of the annual layers is close to 50 µm, the spatial resolution of currently available laboratory-based microtomography systems should be satisfactory to determine the month-at-death [31]. Besides the spatial resolution, the density resolution is critical [32].…”