Laboratory-based phase and absorption tomography for micro-imaging of annual layers in human tooth cementum, paraffin-embedded nerve and zebrafish embryo

Abstract

Synchrotron radiation-based tomography yields the micro-anatomical features in human and animal tissues without physical slicing. Recent advances in instrumentation have made laboratory-based phase tomography feasible. We compared the performance of three cutting-edge laboratory systems benchmarked by synchrotron radiation-based tomography for three specimens. As an additional criterion, the user-friendliness of the three microtomography systems is considered. The three tomography systems-SkyScan 2214 (Bruker-microCT, Kontich, Belgium), Xradia 620 Versa (Zeiss, Oberkochen, Germany) and the prototype of Exciscope (Stockholm, Sweden)-were given 36 hours to measure three medically relevant specimens-archaeological human tooth, porcine nerve and zebrafish embryo. The obtained datasets were registered to the benchmark synchrotron radiation-based tomography from the same specimens and to the SkyScan 1275 and phoenix nanotom m ® laboratory systems to characterize the development during the last decade. The next-generation laboratory-based microtomography almost reached the quality of synchrotron-radiation facilities with respect to spatial and density resolution, as indicated by the visualization of the medically relevant micro-anatomical features. The Exciscope prototype and the SkyScan 2214 demonstrated the complementarity of phase information by imaging the eyes of the zebrafish embryo. The Xradia 620 Versa enabled the three-dimensional visualization of the 2 to 3 µm-thin annual layers in tooth cementum. The data of the Exciscope prototype with the high photon flux from the liquid metal source from Excillum (Kista, Sweden) showed the spiral nature of the myelin sheaths in the porcine nerve. The SkyScan 2214, the most effortable among the three next-generation systems evaluated, was well suited to visualize the wealth of anatomical features in the zebrafish embryo.

Synchrotron X-ray Studies of the Structural and Functional Hierarchies in Mineralised Human Dental Enamel: A State-of-the-Art Review

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Special Section Guest Editorial: Hard X-Ray Tomography with Micrometer Resolution

“…They found that the bioapatite in the cone walls and wedges is oriented to resist lateral and axial deflections, respectively. Based on an awarded conference paper, 5 the article lead-authored by A. Migga systematically compared the performance of three cutting-edge laboratory systems for three specimens namely, paraffin-embedded zebrafish larva, bare archaeological human tooth, and paraffin-embedded porcine nerve ( https://doi.org/10.1117/1.JMI.9.3.031507 ). These systems include SkyScan 2214 (Bruker-microCT, Kontich, Belgium), Exciscope prototype (Stockholm, Sweden), and Xradia 620 Versa (Zeiss, Oberkochen, Germany).…”