Hard Keratin IF and Associated Proteins

Powell, Barry C.; Rogers, George E.

doi:10.1007/978-1-4757-9604-9_10

Cited by 19 publications

(8 citation statements)

References 85 publications

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“…It has been reported that the content of HGT-KAPs is decreased in Merino felting luster (FL) mutant wool that loses crimp [ 17 ], and that the helical angle of IFs in the orthocortex is associated with fiber curvature [ 18 ]. The HGT-KAPs are predominantly present in the orthocortex and are thought to have some associations with the crimp of wool fiber [ 19 ]. The results from this research confirm that variation in KRTAP22-1 may affect those traits.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Ovine Keratin-Associated Protein 22-1 (KAP22-1) Gene and Its Effect on Wool Traits

Zhou

Gong

et al. 2017

Genes

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Keratin-associated proteins (KAPs) are structural components of wool and hair fibers. To date, eight high glycine/tyrosine KAP (HGT-KAP) families have been identified in humans, but only three have been identified in sheep. In this study, the putative ovine homolog of the human KAP22-1 gene (KRTAP22-1) was amplified using primers designed based on a human KRTAP22-1 sequence. Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) was used to screen for variation in KRTAP22-1 in 390 Merino × Southdown-cross lambs and 75 New Zealand (NZ) Romney sheep. Three PCR-SSCP banding patterns were detected and DNA sequencing revealed that the banding patterns represented three different nucleotide sequences (A-C). Two single nucleotide polymorphisms (SNPs) were identified in these sequences. Variant B was most common with a frequency of 81.3% in NZ Romney sheep, while in the Merino × Southdown-cross lambs, A was more common with a frequency of 51.8%. The presence of B was found to be associated with increased wool yield and decreased mean fiber curvature (MFC). Sheep of genotype BB or AB had a higher wool yield than those of genotype AA. These results suggest that ovine KRTAP22-1 variation may be useful when developing breeding programs based on increasing wool yield, or decreasing wool curvature.

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Section: Discussionmentioning

confidence: 99%

Identification of the Ovine Keratin-Associated Protein 22-1 (KAP22-1) Gene and Its Effect on Wool Traits

Zhou

Gong

et al. 2017

Genes

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“…One interesting and useful feature of the IFAP group is that the genes lack introns and are therefore quite small, typically 1 kb or less in size (TABLE 1). 8 When conserved family probes from this group are used on genomic blots of digested sheep DNA, multiple bands, each usually representing a gene, are detected. TABLE 1 lists the known wool keratin gene families and their members.…”

Section: The Number Of Hair Keratin Genesmentioning

confidence: 99%

Regulation of Keratin Gene Expression in Hair Follicle Differentiation^a

Powell

Nesci

Rogers

1991

Annals of the New York Academy of Sciences

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“…The HGT-KAPs are predominantly present in the orthocortex and are thought to have some associations with the crimp of wool fiber (Powell and Rogers, 1990). In the wool follicle, the expression of the HGT-KAPs follows shortly after expression of the keratins, but before the expression of the HS and UHS KAPs (Rogers, 2006).…”

Section: Introductionmentioning

confidence: 99%

Search for Variation in the Ovine KAP7-1 and KAP8-1 Genes Using Polymerase Chain Reaction–Single-Stranded Conformational Polymorphism Screening

Gong

Zhou

Plowman

et al. 2012

DNA and Cell Biology

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Keratins and keratin-associated proteins (KAPs) are large heterogeneous groups of proteins that constitute about 90% of the wool fiber. The genes encoding the high glycine-tyrosine (HGT) KAPs are the first sub-group of KAP genes expressed in the wool follicle and just after expression of the keratin genes. Little is known about variation in these genes, which led us to investigate two HGT-KAP genes, KRTAP7-1 and KRTAP8-1. Polymerase chain reaction-single-stranded conformational polymorphism analysis was used to investigate these genes in 250 Romney-cross sheep. For KRTAP7-1, two unique banding patterns were detected for amplicons that spanned the entire coding region. Sequencing confirmed the presence of two sequences with only one nucleotide difference (c.173G/A) putatively resulting in p.Ser58Asn. One was identical to the published ovine KRTAP7-1 sequence. For KRTAP8-1, five unique banding patterns were detected in an amplicon that spanned the entire coding region. Sequencing revealed five different DNA sequences, all of which were highly homologous to the previously reported ovine KRTAP8-1 sequence. Among these five sequences, four single-nucleotide substitutions were identified and three of them were located in the coding region. One of these was nonsynonymous and would putatively result in p.Tyr34Asn. The variation detected in KRTAP7-1 and KRTAP8-1 may influence their expression or protein structure.

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Hard Keratin IF and Associated Proteins

Cited by 19 publications

References 85 publications

Identification of the Ovine Keratin-Associated Protein 22-1 (KAP22-1) Gene and Its Effect on Wool Traits

Identification of the Ovine Keratin-Associated Protein 22-1 (KAP22-1) Gene and Its Effect on Wool Traits

Regulation of Keratin Gene Expression in Hair Follicle Differentiation^a

Search for Variation in the Ovine KAP7-1 and KAP8-1 Genes Using Polymerase Chain Reaction–Single-Stranded Conformational Polymorphism Screening

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Hard Keratin IF and Associated Proteins

Cited by 19 publications

References 85 publications

Identification of the Ovine Keratin-Associated Protein 22-1 (KAP22-1) Gene and Its Effect on Wool Traits

Identification of the Ovine Keratin-Associated Protein 22-1 (KAP22-1) Gene and Its Effect on Wool Traits

Regulation of Keratin Gene Expression in Hair Follicle Differentiationa

Search for Variation in the Ovine KAP7-1 and KAP8-1 Genes Using Polymerase Chain Reaction–Single-Stranded Conformational Polymorphism Screening

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Regulation of Keratin Gene Expression in Hair Follicle Differentiation^a