Hand-powered centrifugal microfluidic disc with magnetic chitosan bead-based ELISA for antibody quantitation

Lin, Chia Tung; Kuo, Shao Hsuan; Lin, Pei Heng; Chiang, Pei Huan; Wan, Lin; Chang, Chun; Tsou, Ping-Hsien; Li, Bor Ran

doi:10.1016/j.snb.2020.128003

Cited by 37 publications

(23 citation statements)

References 45 publications

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“…However, some lab-based tests rely on bulky and expensive instruments, hindering their widespread use, especially in resource-poor settings where centralized laboratory facilities, funds, and trained personnel are in short supply. Point-of-care testing (POCT), defined restrictively by the College of American Pathologists (CAP) as 'testing provided within the institution, but performed outside the physical facilities of the clinical laboratories, and without the requirement of permanent dedicated space but instead includes kits and instruments, which are either hand carried or transported to the vicinity of patient for immediate testing at that site' [2] , [17] , holds advantages of rapid results, unrestricted test sites and low professional skill requirements for operators [18] , [19] , [20] , [21] . Pushed forward by blazing market demands and the obvious bottlenecks of lab-based tests, POCT has blossomed in many fields such as glucose monitoring and pregnancy tests [22] , [23] , [24] .…”

Section: Introductionmentioning

confidence: 99%

Recent advances and challenges of biosensing in point-of-care molecular diagnosis

Chu

Liu

Liu³

et al. 2021

Sensors and Actuators B: Chemical

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Molecular diagnosis, which plays a major role in infectious disease screening with successful understanding of the human genome, has attracted more attention because of the outbreak of COVID-19 recently. Since point-of-care testing (POCT) can expand the application of molecular diagnosis with the benefit of rapid reply, low cost, and working in decentralized environments, many researchers and commercial institutions have dedicated tremendous effort and enthusiasm to POCT-based biosensing for molecular diagnosis. In this review, we firstly summarize the state-of-the-art techniques and the construction of biosensing systems for POC molecular diagnosis. Then, the application scenarios of POCT-based biosensing for molecular diagnosis were also reviewed. Finally, several challenges and perspectives of POC biosensing for molecular diagnosis are discussed. This review is expected to help researchers deepen comprehension and make progresses in POCT-based biosensing field for molecular diagnosis applications.

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Section: Introductionmentioning

confidence: 99%

Recent advances and challenges of biosensing in point-of-care molecular diagnosis

Chu

Liu

Liu³

et al. 2021

Sensors and Actuators B: Chemical

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“…Such powerful tools are often used in molecular-based diagnoses for infection identification, disease biomarker detection and cancer analysis, which are all strongly correlated with patient treatment selection and timing 4,5 . Point-of-care testing (POCT) is easily deployed in the vicinity of patients outside of the central laboratory and is recognized for providing real-time analysis results 6,7 . Due to their efficiency and convenience, POCT devices directly accelerate therapeutic intervention by capturing personal clinical information in a short amount of time [8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

“…With the increasing threat of pandemic infectious diseases through globalization, it is clear that POCT combined with molecular technologies, such as nucleic acid amplification technique, applied to frontline clinical settings can assist in early-stage implementations for public healthcare 11 . In the development of general public diagnostic applications of POCT, the ideal properties are high sensor performance and low system complexity, which potentially benefit patient management and healthcare, infectious determination, and the limitation of disease spread and ensure low resource consumption and low costs [12][13][14][15][16][17] .…”

Section: Introductionmentioning

confidence: 99%

Passively Driven Microfluidic Device with Simple Operation for Quantitative Droplet LAMP Sssay for Point-of-Care Analysis

Lin

2021

Preprint

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Since polymerase chain reaction (PCR) has become a vital tool for disease diagnosis, the development of precise applied PCR technologies in point-of care testing (POCT) has become more significant. The microfluidic-based PCR platform offers a great opportunity for on-site diagnosis efficiency, and the system is aimed at user-friendly access. Herein, we demonstrate a microfluidic system with simple operation that provides reliable nucleic acid results from 18 uniform droplets via LAMP qPCR detection. By using only micropipette regulation, users are able to control the nanoliter scale of the droplets in this valve-free and pump-free microfluidic (MF) chip. Based on the oil enclosure method and impermeable fabrication, we successfully preserved the reagent inside the microfluidic system, which significantly reduced the fluid loss and condensation. The relative standard deviation (RSD) of the fluorescence intensity between the droplets and during the heating process was <5% and 2.0%, respectively. Additionally, for different nucleic acid detection methods, the MF-LAMP chip in this study showed good applicability to both genome detection and gene expression analysis.

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“…Generally speaking, microfluidics devices are composed of closed channels, and lots of components (e.g., micropumps, microvalves, and other micromixers) are used to assist in completing complex liquid handling tasks. [1][2][3][4][5] By comparison, open-surface microfluidics mainly controls dispersed droplets on the open-surface device by adjusting surface structures and wettability. [6][7][8] In recent years, open-surface microfluidics has attracted much attention due to several advantages, such as reduced the consumption of reagents and samples; 9 a simplified integration processes and controlled system (i.e., no bonding is required); and most importantly, no channels exist, thereby avoiding trapped bubbles and eliminating the risk of the microchannel clogging.…”

Section: Introductionmentioning

confidence: 99%

Rapid construct superhydrophobic microcracks on the open-surface platform for droplet manipulations

Lin

Chen

Wang

et al. 2021

Preprint

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Droplet-based transport driven by surface tension has been explored as an automated pumping source for several biomedical applications. This paper presented a simple and fast superhydrophobic modify and patterning approach to fabricate various open-surface platforms to manipulate droplets to achieve transport, mixing, concentration, and rebounding control. Several commercial reagents were tested in our approach, and the Glaco reagent was selected to create a superhydrophobic layer; laser cutters are utilized to scan on these superhydrophobic surface to create gradient hydrophilic micro-patterns. Implementing back-and-forth vibrations on the predetermined parallel patterns, droplets can be transported and mixed successfully. Colorimetry of horseradish peroxidase (HRP) mixing with substrates also reduced the reaction time by more than 5-times with the help of superhydrophobic patterned chips. Besides, patterned superhydrophobic chips can significantly improve the sensitivity of colorimetric glucose-sensing by more than 10 times. Moreover, all bioassays were distributed homogeneously within the region of hydrophilic micropatterns without the coffee-ring effect. In addition, to discuss further applications of the surface wettability, the way of controlling the droplet impacting and rebounding phenomenon was also demonstrated. This work reports a rapid approach to modify and pattering superhydrophobic films to perform droplet-based manipulations with a lower technical barrier, higher efficiency, and easier operation. It holds the potential to broaden the applications of open microfluidics in the future.

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Hand-powered centrifugal microfluidic disc with magnetic chitosan bead-based ELISA for antibody quantitation

Cited by 37 publications

References 45 publications

Recent advances and challenges of biosensing in point-of-care molecular diagnosis

Recent advances and challenges of biosensing in point-of-care molecular diagnosis

Passively Driven Microfluidic Device with Simple Operation for Quantitative Droplet LAMP Sssay for Point-of-Care Analysis

Rapid construct superhydrophobic microcracks on the open-surface platform for droplet manipulations

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