Recent advances and challenges of biosensing in point-of-care molecular diagnosis

Abstract

Molecular diagnosis, which plays a major role in infectious disease screening with successful understanding of the human genome, has attracted more attention because of the outbreak of COVID-19 recently. Since point-of-care testing (POCT) can expand the application of molecular diagnosis with the benefit of rapid reply, low cost, and working in decentralized environments, many researchers and commercial institutions have dedicated tremendous effort and enthusiasm to POCT-based biosensing for molecular diagnosis. In this review, we firstly summarize the state-of-the-art techniques and the construction of biosensing systems for POC molecular diagnosis. Then, the application scenarios of POCT-based biosensing for molecular diagnosis were also reviewed. Finally, several challenges and perspectives of POC biosensing for molecular diagnosis are discussed. This review is expected to help researchers deepen comprehension and make progresses in POCT-based biosensing field for molecular diagnosis applications.

Diagnostic Accuracy of Liquid Biomarkers in Airway Diseases: Toward Point-of-Care Applications

“…The access of multiplex POC technology will be a gamechanger for chronic and complex disease diagnosis and monitoring. Unfortunately, a drawback of multiplex devices is that they are much more susceptible to cross-contamination, which could severely impair the accuracy of the result ( 171 ). To address this, device architecture can be modified with, for example, hydrophobic barriers that ensure reagents and analytes are restricted to flow along a defined path only that prevents mixing ( 172 , 173 ).…”

Construction of a desirable hyperbolic microfluidic chip for ultrasensitive determination of PCT based on chemiluminescence

“…Generally, PCT can reflect the activity of systemic inflammation reactions, which was a parameter of diagnosis and monitoring bacterial inflammatory disease infection. [33][34][35] Therefore, the DHMC integration with chemiluminescence as signal readout enabled achieving the point-of-care testing (POCT) of PCT in the clinical diagnosis treatment (Scheme 1). Notably, the DHMC by simulation showed a high mixing efficiency for sample reagents, which can then improve the detection sensitivity.…”

An enzyme‐centric approach for constructing an amperometric l‐malate biosensor with a long and programmable linear range

“…Ideal biosensors (such as blood glucose monitors) also offer true “sample in, answer out” operability; that is, no pre‐processing or pre‐treatment of the sample is required. In fields from point‐of‐care diagnostics to food and environmental analyses, it is acknowledged that complicated and/or tedious sample preparation can decrease real‐world uptake of new biosensor technologies and increase the errors introduced by minimally trained users (Ashley et al, 2017; Campuzano et al, 2020; Chu et al, 2021; Park et al, 2020; Ribeiro et al, 2014).…”