Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico.

Montalvo‐Rodríguez, Rafael; López‐Garriga, Juan; Vreeland, Russell H.; Oren, Aharon; Ventosa, António; Kamekura, Masahiro

doi:10.1099/00207713-50-3-1065

Cited by 65 publications

(56 citation statements)

References 34 publications

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“…T was 68.1 mol%, which is higher than that of Haloterrigena thermotolerans PR5 T (63.3 mol%) (Montalvo- Rodríguez et al, 2000) in the family Natrialbaceae and similar to that of Halobacterium jilantaiense NG4…”

Section: Cells Of Strain Cba1114mentioning

confidence: 71%

Halostella salina gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt

Song

Tae

Rhee

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Halostella salina gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt . In multilocus sequence analysis (MLSA), five housekeeping genes, atpB, EF-2, radA, rpoB¢ and secY, were found to be closely related to those of the members of the genera Halorientalis(89.7 % similarity of the atpB gene sequence), Halomicroarcula(91.9 %, EF-2), Haloterrigena(85.4 %, radA), Natronoarchaeum (89.2 %, rpoB¢) and Natrinema(75.7 %, secY). A phylogenetic tree generated from the results of MLSA of the five housekeeping genes showed that strain CBA1114T was closely related to species of the genus Halorientalis in the family Halobacteriaceae. The major polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and unidentified lipids. The G+C content of the genomic DNA of strain CBA1114 T was 68.1 mol%. According to the results of phylogenetic, phenotypic and chemotaxonomic analyses, we designate strain CBA1114 T (=JCM 30111 T =KCTC 4206 T ) as the type strain of Halostella salina gen. nov., sp. nov., a novel species of a new genus within the family Halobacteriaceae.

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Section: Cells Of Strain Cba1114mentioning

confidence: 71%

Halostella salina gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt

Song

Tae

Rhee

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…DNase activity was detected on T-Hv agar medium as described by González et al (1978). Utilization of sole carbon and energy sources, and acid production from sugars were determined as described by Montalvo-Rodríguez et al (2000). Anaerobic growth in the presence of nitrate, sulfate, thiosulfate, DMSO and TMAO was tested in filled, stoppered tubes with Sehgal-Gibbons (SG) medium (Sehgal & Gibbons, 1960) for 1 month.…”

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confidence: 99%

“…Since the establishment of the genus Haloterrigena within the family Halobacteriaceae in 1999 (Ventosa et al, 1999), at the time of writing seven species belonging to it have been described: Haloterrigena turkmenica (Ventosa et al, 1999), Haloterrigena thermotolerans (Montalvo-Rodríguez et al, 2000), Haloterrigena saccharevitans (Xu et al, 2005a), Haloterrigena longa and Haloterrigena limicola (Cui et al, 2006), Haloterrigena hispanica (Romano et al, 2007) and Haloterrigena salina (Gutiérrez et al, 2008). To further increase our understanding of the family Halobacteriaceae, we searched for novel strains of this family in shrimp jeotgal, a traditional fermented seafood from Korea that comprises small shrimps and rock salt.…”

mentioning

confidence: 99%

“…It is known that the genera Haloterrigena and Natrinema overlap phylogenetically to some extent, and detailed taxonomic reassessment studies have been carried out (Montalvo-Rodríguez et al, 2000;Oren & Ventosa, 2002). Currently, it is reported that Haloterrigena species contain the glycolipids S 2 -DGD (Cui et al, 2006;Gutiérrez et al, 2008;Montalvo-Rodríguez et al, 2000;Ventosa et al, 1999;Xu et al, 2005a) or mannose-6-sulfate(1-2)-glucose glycerol diether (S-DGD) (Romano et al, 2007), whereas most Natrinema species have phosphatidylglycerol sulfate (PGS) and unidentified glycolipids (McGenity et al, 1998;Tapingkae et al, 2008;Xin et al, 2000;Xu et al, 2005b).…”

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confidence: 99%

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Haloterrigena jeotgali sp. nov., an extremely halophilic archaeon from salt-fermented food

Roh

Nam

Chang

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A novel red-pigmented halophilic archaeon, strain A29T , was isolated from shrimp jeotgal, a traditional salt-fermented food from Korea. This strain grows in the ranges 10-30 % (w/v) NaCl, 17-50 6C and pH 6.5-8.5, with optimal growth occurring at 15-20 % NaCl, 37-45 6C and pH 7.0-7.5. The isolate is Gram-negative and non-motile. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain A29 T is associated with the genus Haloterrigena and closely related to the species Haloterrigena thermotolerans (99.0 % similarity). However, DNA-DNA hybridization experiments revealed that the level of hybridization between strain A29 T and related strains of Haloterrigena is less than 70 %. The polar lipid fraction consists of phosphatidylglyerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me) and mannose-2,6-disulfate(1-2)-glucose glycerol diether (S 2 -DGD). The G+C content of genomic DNA of the type strain is 62.3 mol%. On the basis of this polyphasic taxonomic study, strain A29 T should be placed in the genus Haloterrigena as a novel species, for which the name Haloterrigena jeotgali sp. nov. is proposed. The type strain of the new species is A29 T (5KCTC 4020 T 5DSM 18794T 5JCM 14585 T 5CECT 7218 T ).Although high salinity is toxic to most cells, extreme halophiles are well adapted to their hypersaline environment (Grant, 2004). Since the establishment of the genus Haloterrigena within the family Halobacteriaceae in 1999 (Ventosa et al., 1999), at the time of writing seven species belonging to it have been described: Abbreviations: S-DGD, mannose-6-sulfate(1-2)-glucose glycerol diether; S 2 -DGD, mannose-2,6-disulfate(1-2)-glucose glycerol diether.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A29T is EF077633.Phylogenetic trees based on 16S rRNA gene sequences generated by the minimum-evolution and maximum-parsimony methods, and a thinlayer chromatogram of the polar lipids of strain A29 T and a reference strain (H. thermotolerans PR5 , 1997). Cell morphology was examined using light (Eclipse 80i; Nikon) and electron microscopy (JEM 1010; JEOL). Gram staining was performed as described by Dussault (1955) and motility was examined on semi-solid agar. Optimal conditions for growth were determined in medium 954 containing 0-30 % (w/v) NaCl, and the pH range for growth was assayed from pH 5.0 to 11.0 at intervals of 0.5, and at different temperatures (4, 10, 15, 17, 21, 25, 30, 37, 45, 50 and 60 u C). The Mg 2+ requirement for growth was also determined using medium 954 without MgCl 2 . 6H 2 O and yeast extract. Standard phenotypic tests for indole formation, nitrate reduction, hydrolysis of casein, starch and urea, and activity of oxidase and catalase were conducted as described by Gerhardt et al. (1994). Hydrolysis of gelatin and Tween 80 were tested simultaneously through the procedure adapted for haloarchaea by Gutiérrez & González (1972). Cell lysis in distilled water was detected by microscopic examination. DNase activity was detected on T-Hv agar medium as...

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“…Macroscopic properties were documented by using the classical characterization of colony appearance. Optimal conditions for growth were determined by growing strain MS10 T in SG broth supplemented with 0, 5, 10, 15, 20, 25 and 30 % (w/v) NaCl and at temperatures of 10,15,20,25,30,33,35,40 and 50 u C. The pH range for growth was tested in SG medium with 10 % NaCl adjusted to pH values of 4.0, 5.0, 6.0, 7.0, 8.0, 9.0 and 10.0 with the addition of the appropriate buffering ability to each medium as described by Montalvo-Rodríguez et al (2000). Cells were cultivated in SG medium with constant agitation (150 r.p.m.)…”

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confidence: 99%

Halobacillus mangrovi sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicennia germinans

Soto-Ramírez

Sánchez‐Porro

Rosas-Padilla

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A moderately halophilic, spore-forming, Gram-positive, short-rod-shaped bacterium, designated strain MS10 T , was isolated from the surface of leaves of the black mangrove Avicennia germinans and was subjected to a polyphasic taxonomic study. Strain MS10 T was able to grow at NaCl concentrations in the range 5-20 % (w/v) with optimum growth at 10 % (w/v) NaCl. Growth occurred at temperatures of 10-50 6C (optimal growth at 33-35 6C) and pH 6.0-9.0 (optimal growth at pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MS10 T fell within the branch encompassing members of the genus Halobacillus and was most closely related to Halobacillus dabanensis JCM 12772 T (99.2 % 16S rRNA gene sequence similarity). The DNA G+C content of strain MS10 T was 45.7 mol%, the major respiratory isoprenoid quinone was MK-7 and the cell-wall peptidoglycan was of the L-Orn-D-Asp type, characteristics consistent with its affiliation to the genus Halobacillus. Strain MS10 T showed a level of DNA-DNA hybridization with H. dabanensis JCM 12772 T of 29 % and levels below 70 % were also obtained with respect to other recognized members of the genus Halobacillus. The major fatty acids of strain MS10 T were iso-C 16 : 0 , anteiso-C 15 : 0 , iso-C 14 : 0 and iso-C 15 : 0 . Overall, the phenotypic, genotypic and phylogenetic results presented in this study demonstrate that strain MS10 T represents a novel species of the genus Halobacillus, for which the name Halobacillus mangrovi sp. nov. is proposed. The type strain is MS10 T (5CECT 7206 T 5CCM 7397 T ).The genus Halobacillus was first described by Spring et al. (1996) et al., 2005). Besides the ability to produce ellipsoidal or spherical endospores at the central or subterminal position, one of the main features of the genus Halobacillus is the cell-wall peptidoglycan type based on L-Orn-D-Asp, in contrast to the meso-diaminopimelic acid or L-lysine type cell-wall peptidoglycan found in related genera (Shida et al., 1997;Yoon et al., 2003). There have been no previous reports of members of the genus Halobacillus associated with mangroves that thrive in saline habitats.Avicennia germinans (black mangrove) is a type of mangrove that has developed mechanisms to tolerate high salt concentrations in unstable substrates, to obtain oxygen from almost anoxic sediments and to reproduce under harsh environmental conditions. One of these mechanisms is the ability to secrete salt crystals on to the surface of its leaves when the salt concentration is in excess of that normally present in the vascular system (Lugo & Snedaker, 1975). In the present study, we determined the taxonomic position of bacterial strain MS10 T , which was isolated from the surface of leaves of the black mangrove during a microbial diversity study. On the basis of the results presented, we show that strain MS10 T represents a novel species of the genus Halobacillus.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain MS10 T is DQ888316. & Gibbons, 1960). This p...

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Haloterrigena thermotolerans sp. nov., a halophilic archaeon from Puerto Rico.

Cited by 65 publications

References 34 publications

Halostella salina gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt

Halostella salina gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt

Haloterrigena jeotgali sp. nov., an extremely halophilic archaeon from salt-fermented food

Halobacillus mangrovi sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicennia germinans

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