Halostella salina gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt

Abstract

Halostella salina gen. nov., sp. nov., an extremely halophilic archaeon isolated from solar salt . In multilocus sequence analysis (MLSA), five housekeeping genes, atpB, EF-2, radA, rpoB¢ and secY, were found to be closely related to those of the members of the genera Halorientalis(89.7 % similarity of the atpB gene sequence), Halomicroarcula(91.9 %, EF-2), Haloterrigena(85.4 %, radA), Natronoarchaeum (89.2 %, rpoB¢) and Natrinema(75.7 %, secY). A phylogenetic tree generated from the results of MLSA of the five housekeeping genes showed that strain CBA1114T was closely related to species of the genus Halorientalis in the family Halobacteriaceae. The major polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and unidentified lipids. The G+C content of the genomic DNA of strain CBA1114 T was 68.1 mol%. According to the results of phylogenetic, phenotypic and chemotaxonomic analyses, we designate strain CBA1114 T (=JCM 30111 T =KCTC 4206 T ) as the type strain of Halostella salina gen. nov., sp. nov., a novel species of a new genus within the family Halobacteriaceae.

Diversity of Extremely Halophilic Archaeal and Bacterial Communities from Commercial Salts

“…It is a well-known property of many halophiles to be polyploid and also to harbor multiple unrelated 16S rRNA gene copies in one cell (Oren, 2012 ). Our previous studies on haloarchaeal taxonomy also revealed that the haloarchaeal taxa such as Halostella salina (Song et al, 2016 ), Halapricum salinum (Song et al, 2014 ), and Halorubrum halophilum (Yim et al, 2014 ), have at least two different copies of the 16S rRNA gene. Because it is accurate to consider all of the reported heterogeneous 16S rRNA gene sequences for the phylogenetic study of the haloarchaea, we used the EzTaxon-e database containing the unrelated 16S rRNA gene copies of the haloarchaea to analyze haloarchaeal community profiles in the commercial salt samples.…”

The human gut archaeome: identification of diverse haloarchaea in Korean subjects

“…Information regarding the community-based genetic and functional traits of archaea in animal habitats is scarce. We have previously reported occurrences of diverse members of extremely halophilic archaea (haloarchaea) in avian plumage [13], as well as in food samples such as salt-fermented seafood [14] and solar salts [15,16]. In the human gut where the microbial entities thrive more abundantly than in other parts of the human body, the archaeome consisted mostly of methane-producing archaea (methanogens), of which, members belonging to the orders Methanobacteriales (including Methanobrevibacter smithii and Methanosphaera stadtmanae) and Methanomassiliicoccales (including Methanomethylophilaceae) are predominant [17].…”

The human gut archaeome: identification of diverse haloarchaea in Korean subjects

“…Information regarding the community-based genetic and functional traits of archaea in animal habitats is scarce. We have previously reported occurrences of diverse members of extremely halophilic archaea (haloarchaea) in avian plumage [13], as well as in food samples such as salt-fermented seafood [14] and solar salts [15,16]. In the human gut where the microbial entities thrive more abundantly than in other parts of the human body, the archaeome consisted mostly of methane-producing archaea (methanogens), of which, members belonging to the orders Methanobacteriales (i.e.…”