Halotalea alkalilenta gen. nov., sp. nov., a novel osmotolerant and alkalitolerant bacterium from alkaline olive mill wastes, and emended description of the family Halomonadaceae Franzmann et al. 1989, emend. Dobson and Franzmann 1996

Abstract

Halotalea alkalilenta gen. nov., sp. nov., a novel osmotolerant and alkalitolerant bacterium from alkaline olive mill wastes, and emended description of the family Halomonadaceae Franzmann et al. 1989, emend. Dobson andFranzmann 1996 A novel Gram-negative, motile, nonsporulating, rod-shaped bacterium isolated from alkaline sludge-like wastes ('alpeorujo' or 'alperujo') of two-phase olive oil extraction is described. The strain, designated AW-7 T , is an obligate aerobe that is halotolerant (tolerating up to 15 % w/v NaCl), sugar-tolerant (tolerating up to 45 % and 60 % w/v (+)-D-glucose and maltose respectively; these are the highest concentrations tolerated by any known members of the Bacteria domain) and alkalitolerant (growing at a broad pH range of 5-11). Strain AW-7 T is chemo-organotrophic. Ubiquinone-9 was detected in the respiratory chain of strain AW-7 T . The major fatty acids present are C 18 : 1 v7c, C 16 : 0 , C 19 : 0 cyclo v8c, C 12 : 0 3-OH and C 16 : 1 v7c/iso-C 15 : 0 2-OH. Based on 16S rRNA gene sequence analysis, strain AW-7 T showed almost equal phylogenetic distances from Zymobacter palmae (95.6 % similarity) and Carnimonas nigrificans (95.4 % similarity). In addition, low DNA-DNA relatedness values were found for strain AW-7 T against Carnimonas nigrificans CECT 4437 T (22.5-25.4 %) and Z. palmae DSM 10491 T (11.9-14.4 %). The DNA G+C content of strain AW-7 T is 64.4 mol%. Physiological and chemotaxonomic data further confirmed the differentiation of strain AW-7 T from the genera Zymobacter and Carnimonas. Thus, strain AW-7 T represents a novel bacterial genus within the family Halomonadaceae, for which the name Halotalea gen. nov. is proposed. Halotalea alkalilenta sp. nov. (type strain AW-7 T 5DSM 17697 T 5CECT 7134 T ) is proposed as the type species of the genus Halotalea gen. nov. A reassignment of the descriptive 16S rRNA signature characteristics of the family Halomonadaceae permitted the placement of the novel genus Halotalea into the family; in contrast, the genus Halovibrio possessed only 12 out of the 18 signature characteristics proposed, and hence it was excluded from the family Halomonadaceae.

Halomonas garicola sp. nov., isolated from saeu-jeot, a Korean salted and fermented shrimp sauce

“…At the time of writing, the family Halomonadaceae belonging to the class Gammaproteobacteria consists of nine halophilic genera with validly published names (Aidingimonas, Chromohalobacter, Cobetia, Halomonas, Halotalea, Kushneria, Modicisalibacter, Salinicola and Larsenimonas) (Vreeland et al, 1980;Ventosa et al, 1989;Mellado et al, 1995;Arahal et al, 2002;Arahal & Ventosa, 2006;Gam et al, 2007;Ntougias et al, 2007;Sánchez-Porro et al, 2009;Wang et al, 2009;Leó n et al, 2015) that are capable of growth in saline environments with 15 % (w/v) salt concentrations plus two nonhalophilic genera (Carnimonas and Zymobacter) (Okamoto et al, 1993;Garriga et al, 1998). Among the genera, the genus Halomonas including Gram-stain-negative, moderately halophilic, aerobic or facultatively aerobic rod-shaped bacteria is the largest genus, which includes 90 species with validly published names that have been isolated from diverse saline environments such as solar salterns, salt lakes, fermented seafood, sea squirts, sea ice, hydrothermal vents and saline-alkali soil (Vreeland et al, 1980;Ventosa et al, 1998;Romanenko et al, 2002;Yoon et al, 2002;Kaye et al, 2004;Lim et al, 2004;Arenas et al, 2009;Kim et al, 2010;Guan et al, 2010;Guzmán et al, 2010;Jeong et al, 2013;Miao et al, 2014).…”

Halomonas fontilapidosi sp. nov., a moderately halophilic, denitrifying bacterium

“…Chromohalobacter, Cobetia, Halomonas and Modicisalibacter are composed of halophilic bacteria whereas Carnimonas, Halotalea and Zymobacter contain non-halophilic bacteria (Ben Ali Gam et al, 2007;Garrity et al, 2005;Ntougias et al, 2007). The genus Halomonas currently contains more than 50 species (Euzéby, 2008).…”

Halomonas xinjiangensis sp. nov., a halotolerant bacterium isolated from a salt lake

“…The G+C content of the genomic DNA was 60.0 mol%. The family Halomonadaceae currently includes four genera of halophilic bacteria, Halomonas, Chromohalobacter, Cobetia and Modicisalibacter, and three genera of nonhalophilic bacteria, Carnimonas, Halotalea and Zymobacter (Franzmann et al, 1988;Ventosa et al, 1989;Okamoto et al, 1993;Dobson & Franzmann, 1996;Garriga et al, 1998;Arahal et al, 2002Arahal et al, , 2007Ntougias et al, 2007; Ben Ali Gam et al, 2007). The genus Halomonas was proposed by Vreeland et al (1980), with Halomonas elongata as the type species.…”