Halococcus dombrowskii sp. nov., an archaeal isolate from a Permian alpine salt deposit.

Stan‐Lotter, Helga; Pfaffenhuemer, M.; Legat, Andrea; Busse, Hans‐Jürgen; Radax, Christian; Gruber, Claudia

doi:10.1099/00207713-52-5-1807

Cited by 57 publications

(75 citation statements)

References 44 publications

(48 reference statements)

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“…Determination of antibiotic susceptibility was tested according to the methods of Stan-Lotter et al (2002). Menaquinones were analysed as described by Komagata & Suzuki (1987).…”

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confidence: 99%

Halobacterium piscisalsi sp. nov., from fermented fish (pla-ra) in Thailand

Yachai

Tanasupawat

Itoh

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A Gram-negative, motile, rod-shaped, extremely halophilic archaeon, designated strain HPC1-2(T), was isolated from pla-ra, a salt-fermented fish product of Thailand. Strain HPC1-2(T) was able to grow at 20-60 degrees C (optimum at 37-40 degrees C), at 2.6-5.1 M NaCl (optimum at 3.4-4.3 M NaCl) and at pH 5.0-8.0 (optimum at pH 7.0-7.5). Hypotonic treatment with less than 1.7 M NaCl caused cell lysis. The major polar lipids of the isolate were C(20)C(20) derivatives of phosphatidylglycerol, methylated phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether, sulfated triglycosyl diether and sulfated tetraglycosyl diether. The G+C content of the DNA was 65.5 mol%. 16S rRNA gene sequence analysis indicated that the isolate represented a member of the genus Halobacterium in the family Halobacteriaceae. Based on 16S rRNA gene sequence similarity, strain HPC1-2(T) was related most closely to Halobacterium salinarum DSM 3754(T) (99.2%) and Halobacterium jilantaiense JCM 13558(T) (97.8%). However, low levels of DNA-DNA relatedness suggested that strain HPC1-2(T) was genotypically different from these closely related type strains. Strain HPC1-2(T) could also be differentiated based on physiological and biochemical characteristics. Therefore, strain HPC1-2(T) is considered to represent a novel species of the genus Halobacterium, for which the name Halobacterium piscisalsi sp. nov. is proposed. The type strain is HPC1-2(T) (=BCC 24372(T)=JCM 14661(T)=PCU 302(T)).

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“…Determination of antibiotic susceptibility was tested according to the methods of Stan-Lotter et al (2002). Menaquinones were analysed as described by Komagata & Suzuki (1987).…”

mentioning

confidence: 99%

Halobacterium piscisalsi sp. nov., from fermented fish (pla-ra) in Thailand

Yachai

Tanasupawat

Itoh

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…4, A1, B1, B2), where differences in one-and two-dimensional protein patterns were apparent. Halococci possess acidic proteins with a rather narrow range of pIs between 4.2 and 5.2 (Stan-Lotter et al, 2002), which tend to cluster on two-dimensional gels. However, separation of spots on our two-dimensional gels was adequate (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Cells of Halococcus dombrowskii DSM 14522 T and Haloferax mediterranei DSM 1411 T were grown, with shaking, in M2 medium (Tomlinson and Hochstein, 1976) in liquid culture as described previously (Stan-Lotter et al, 2002). Growth was followed by measuring optical density (OD) at 600 nm with a Novaspec II (Pharmacia).…”

Section: Growth Of Microorganismsmentioning

confidence: 99%

“…For example, Halorubrum chaoviator strain Halo-G* (Mancinelli et al, 2009) was flown on the Biopan facility, a small retrievable capsule developed by the European Space Agency for exposure of biological samples in low-Earth orbit (ESA, 2005), and survived exposure to outer space for 2 weeks (Mancinelli et al, 1998). Halococcus dombrowskii, an isolate from Permian salt sediments (Stan-Lotter et al, 2002), was included in the Adapt experiment on the International Space Station and was exposed to the space environment for 18 months (http://www.esa.int/esaHS/SEM9X9W0EZF_iss_0 .html). For the exposure experiments, suspensions of haloarchaeal cells in high-salt medium or buffers are dried on quartz discs, which leads to the formation of fluid inclusions during crystallization.…”

Section: Introductionmentioning

confidence: 99%

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Responses of Haloarchaea to Simulated Microgravity

Dornmayr-Pfaffenhuemer

Legat

Schwimbersky

et al. 2011

Astrobiology

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Various effects of microgravity on prokaryotes have been recognized in recent years, with the focus on studies of pathogenic bacteria. No archaea have been investigated yet with respect to their responses to microgravity. For exposure experiments on spacecrafts or on the International Space Station, halophilic archaea (haloarchaea) are usually embedded in halite, where they accumulate in fluid inclusions. In a liquid environment, these cells will experience microgravity in space, which might influence their viability and survival. Two haloarchaeal strains, Haloferax mediterranei and Halococcus dombrowskii, were grown in simulated microgravity (SMG) with the rotary cell culture system (RCCS, Synthecon). Initially, salt precipitation and detachment of the porous aeration membranes in the RCCS were observed, but they were avoided in the remainder of the experiment by using disposable instead of reusable vessels. Several effects were detected, which were ascribed to growth in SMG: Hfx. mediterranei's resistance to the antibiotics bacitracin, erythromycin, and rifampicin increased markedly; differences in pigmentation and whole cell protein composition (proteome) of both strains were noted; cell aggregation of Hcc. dombrowskii was notably reduced. The results suggest profound effects of SMG on haloarchaeal physiology and cellular processes, some of which were easily observable and measurable. This is the first report of archaeal responses to SMG. The molecular mechanisms of the effects induced by SMG on prokaryotes are largely unknown; haloarchaea could be used as nonpathogenic model systems for their elucidation and in addition could provide information about survival during lithopanspermia (interplanetary transport of microbes inside meteorites).

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“…Phenotypic characterization (Table 3) as well as protein electrophoresis profiles and the plasmid characterization show distinct differences between the four Haloarcula-like isolates and the three Halorubrum isolates. PAGE of cellular proteins has been found useful for preliminary clustering of unknown halophilic organisms (Hesselberg & Vreeland 1995), and has been used to identify related species (Stan-Lotter et al 2002). Unfortunately, protein profile data of new isolates have seldom been included in species descriptions (Kamekura & Dyall-Smith 1995).…”

Section: Discussionmentioning

confidence: 99%