Characterization of extremely halophilic Archaea isolated from the Ayvalik Saltern, Turkey

Elevi, Rahel; Assa, Parvaneh; Birbir, Meral; Ogan, Ayşe; Oren, Aharon

doi:10.1007/s11274-004-4515-z

Cited by 45 publications

(37 citation statements)

References 30 publications

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“…The low pH of this wetland makes it distinct from other hypersaline environments of Iran like Howz-Soltan and Aran-Bidgol lakes with high salinity and neutral pH and Gomishan wetland with low salinity and high pH. In comparison with Tuz lake in Turkey [18] and Marras lake in Peru [19]. The amount of Mg 2+ ion of this wetland was lower and also in comparison with other hypersaline lakes of Iran, the amount of Mg 2+ in Urmia lake and Aran-Bidgol lake was in order 3.5 and 1.5 times higher than IncheBroun wetland.…”

Section: Discussionmentioning

confidence: 83%

“…Among the strains belonged to archaea most of them were similar to members of Haloarcula and Halorubrum(in order 30 and 27.5 percents of the isolates). Results of other surveys performed in other hypersaline regions of the world shows that: members of Halorubrum and Haloarculaare the main groups of isolates from Tuz salt lake in Turkey [18], members of Haloarculaconstitue 50 percent of the isolates of Maras lake in Peru [18], in solar slatterns of Australia more than 70 percent of the isolates belonged to genus Halorubrum and in Ayakekumu salt lake in China 47 percent of isolates belonged to genus Halorubrum and 24 percents were belonged to Natrinema [27]. In surveys performed in Aran-Bigol salt lake in Iran the archaeal isolates were similar to members of Halorubrum, Haloarcula, Natrinema, Halogeometricum, Natronomonas, Halobacterium, Halovivax, Halolamina and Halorientalis [9].…”

Section: Discussionmentioning

confidence: 95%

“…Eleviet al (2004) studied the production of amylase, protease and lipase in halo archaea. They reported a high rate of amylase activity but neither protease nor lipase activity was observed [18]. Among bacterial strains only lipase activity was observed in strains similar to members of Psuedomonas and Salicola.…”

Section: Volume 56mentioning

confidence: 94%

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Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Rasooli

Amoozegar

Sepahy

et al. 2016

ILNS

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Abstract. Extremely halophilic diversity of IncheBroun wetland located in the north of Iran was investigated by using culture-dependent methods. Sampling was carried out in May and September 2014. In each sampling 4 distinct regions of wetland were analyzed by using complex media like MGM, JCM168, MH1 and an alkaliphilic medium containing 23% salts. After incubation at 40˚C, a total of 406 isolates and 2.1 × 10 6 CFU/ml were obtained in culture media. Among them 361 isolates were obtained from MGM and 39 isolates from JCM 168, 3 isolates from MH1 and 3 isolates from the alkaliphilic media. Initial morphological, biochemical and physiological tests were performed. Production of 4 hydrolytic enzymes by 45 selected strains was assayed qualitatively. A total of 38, 19 and 6 strains were able to produce lipase, DNase and amylase activity. Protease activity was not observed among strains. As total 45 strains were selected randomly and phylogenetic analysis of 16S rRNA was performed for them. Among selected strains 40 isolated strians belonged to Haloarchaea and were belonged to the genera: Haloarcula(30%), Halorubrum(27.5%), Haloferax(17.5%), Halobellus (10%), Halogeometricum(5.2%), Halobacterium(2.6%), Halolamina(2.6%), Halorhabdus (2.6%) and Halostagnicola (2.6%). Haloarcula and Halorubrum were the dominant populations. A total of 5 strains belonged to domain of Bacteria and were similar to members of Rhodovibrio (40%), Pseudomonas (40%) and Salicola (20%).

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 95%

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Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Rasooli

Amoozegar

Sepahy

et al. 2016

ILNS

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“…31 Birbir and Sesal 32 studied the extreme halophilic bacterial communities of Sereflikochisar salt lakes of Turky by using conventional biochemical features, while Birbir et al 33 characterized extremophilic communities in Tuzkoy salt mine and the adjacent Kaldirim and Kayacik salterns using positive PCR amplification and denaturing gradient gel electrophoresis (DGGE) analysis of DNA sequences which revealed the phylogenetic inclusion of the isolated strains within the genera Halobacterium, Haloarcula, Natrinema and Halorubrum. Elvi et al 34 isolated extremely halophilic strains from the Ayvalik saltern in the north-eastern part of Turkey. Similarly, Enache et al 35 isolated extreme halophiles from Telega salt lake, of Romania and identified them as members of the genus Haloferax.…”

Section: Diversity Of Halophilesmentioning

confidence: 99%

Diversity and Production of Extracellular Polysaccharide by Halophilic Microorganisms

Ak¹

2017

BIJ

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Halophilic microorganisms derived from diverse thalassohaline and athalassohaline environments including marine estuaries, saline and soda lakes, inland solar salterns and acidic habitats are categorized as slight-, moderate-and extreme halophiles according to their NaCl requirements. Taxonomic studies with culturable diversities of halophiles revealed that they belong to both Archaea and Bacteria representing the families Halobacteriaceae, Methanosarcinaceae and the class Gammaproteobacteria. As adaptive strategies against harsh salt stresses, majority of halophiles often synthesize and accumulate extracellular polysaccharides (EPS) which differ significantly in terms of their physical, chemical and material properties. So far the novelty in structure and functions of exopolysaccharides are concerned, producer strains belonging to the genera Halomonas and Haloferax have attracted the main attention. However, EPS producing strains belonging to the genera Idiomarina, Salipiger and Alteromonas are not uncommon. Through process optimization and metabolic regulation a number of potent halophilic strains have been found to produce copious amount of EPS indicating its commercial viability. Moreover, the significance of production, physico-chemical and biological properties along with the possible applications of halophilic EPS in industry and biotechnology have also been highlighted..

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“…The protease growth medium contained (g L -l ) the following: NaCl 25, KCl 2, MgSO4 20, tri-sodium citrate 3, yeast extract 10, agar 20, pH 7.2 (Elevi et al, 2004). As a preparation, solid medium salts were separately autoclaved at 121°C for 15 min, cooled and then mixed with warm yeast extract-agar mixture to avoid precipitation of medium components.…”

Section: Screening Of Extracellular Proteasementioning

confidence: 99%

Halophile isolation to produce halophilic protease, protease production and testing crude protease as a detergent ingredient

Sekar¹,

Packyam²,

Kim³

2016

Afr. J. Microbiol. Res.

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Halophilic enzymes are potentially useful in many industries, particularly in food fermentation, pharmaceutical, textile, and leather for the treatment of saline and hypersaline wastewaters. In this study, a halophilic bacterium was isolated from saltpan environment, and was identified as Bacillus sp. Mk22 through biochemical test and 16S rRNA gene sequencing. During protease screening, the isolates produced 24 mm clear zone around the bacterial colony. The maximum production of proteases was due to the following conditions: 45°C, pH 8, 12% NaCl, carbon source glucose, nitrogen source skim milk powder and 42-h culture time, respectively. The protein was purified 16.5 fold, having 24.01% recovery, in DEAE-cellulose chromatography and 64 kDa molecular weight. Ca and Zn enhanced protease activity, while Hg strongly inhibited it. The protease was used to destain blood, ink, coffee and was active and stable under more than one extreme condition of high salt, pH, and temperature.

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Characterization of extremely halophilic Archaea isolated from the Ayvalik Saltern, Turkey

Cited by 45 publications

References 30 publications

Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Isolation, Identification and Extracellular Enzymatic Activity of Culturable Extremely Halophilic Archaea and Bacteria of IncheBoroun Wetland

Diversity and Production of Extracellular Polysaccharide by Halophilic Microorganisms

Halophile isolation to produce halophilic protease, protease production and testing crude protease as a detergent ingredient

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