Halilectin 1 (H‐1) and Halilectin 2 (H‐2): two new lectins isolated from the marine sponge <i>Haliclona caerulea</i>

Carneiro, Rômulo Farias; Melo, Arthur A.; Nascimento, Fernando Edson Pessoa do; Simplicio, Clareane Avelino; Nascimento, Kyria S.; Rocha, B.A.M.; Saker-Sampaio, Silvana; Moura, Raniere da Mata; Mota, Sula Salani; Cavada, Benildo S.; Nagano, Celso Shiniti; Sampaio, Alexandre Holanda

doi:10.1002/jmr.2243

Cited by 21 publications

(20 citation statements)

References 37 publications

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“…Similar to lectins isolated from Haliclona caerulea (Carneiro et al 2013), Axinella corrugata (Dresch et al 2011) and Aplysia kurodai (Kawsar et al 2010), HGL showed a high level of cytotoxicity against Artemia nauplii. However, hemolysis caused by lectin is rare, and few lectins have shown hemolytic activity (Hatakeyama et al 1994, Sudhakar andVincent 2014).…”

Section: Discussionmentioning

confidence: 61%

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Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea

Moura

Melo

Carneiro

et al. 2015

An. Acad. Bras. Ciênc.

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Twenty species of marine invertebrates from the Brazilian coast were screened for hemagglutinating/ hemolytic activity. In at least twelve tested species, hemagglutinating activity was different for different blood types, suggesting the presence of lectins. Extracts from four species showed hemolytic activity. Two new lectins were purified from the marine sponge Cliona varians (CvL-2) and sea cucumber Holothuria grisea (HGL). CvL-2 was able to agglutinate rabbit erythrocytes and was inhibited by galactosides. The hemagglutinating activity was optimal in pH neutral and temperatures below 70 °C. CvL-2 is a trimeric protein with subunits of 175 kDa. On the other hand, HGL showed both hemagglutinating and hemolytic activity in human and rabbit erythrocytes, but hemolysis could be inhibited by osmotic protection, and agglutination was inhibited by mucin. HGL was stable in pH values ranging from 4 to 10 and temperatures up to 90 °C. In electrophoresis and gel filtration, HGL was a monomeric protein with 15 kDa. CvL-2 and HGL showed different levels of toxicity to Artemia naplii. CvL-2 showed LC 50 of 850.1 μg/mL, whereas HGL showed LC 50 of 9.5 µg/mL.

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Section: Discussionmentioning

confidence: 61%

“…Lethality test on Artemia nauplii was performed in accordance to pre-established methods (Carneiro et al 2013).…”

Section: Lethality Test In Artemiamentioning

confidence: 99%

Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea

Moura

Melo

Carneiro

et al. 2015

An. Acad. Bras. Ciênc.

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“…The artemia lethality test was conducted according to Carneiro et al (2013). The Artemia cysts were hatched in artificial seawater at 28ºC under constant lighting and strong aeration.…”

Section: Artemia Lethality Testmentioning

confidence: 99%

Antioxidant, hemolytic, antimicrobial, and cytotoxic activities of the tropical Atlantic marine zoanthid Palythoa caribaeorum

Alencar

Melo

Silva

et al. 2015

An. Acad. Bras. Ciênc.

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Marine invertebrates are capable of synthesizing bioactive compounds, which may be beneficial to human health. The aim of this study was to evaluate the antioxidant, hemolytic, antimicrobial and cytotoxic activities of crude extract (70% EtOH), and dichloromethane (DCM), ethyl acetate (EtOAc), and aqueous (Aq) fractions of the marine zoanthid Palythoa caribaeorum. The phenolic compound contents of the crude extract, DCM, EtOAc and Aq fractions were 12.33, 18.17, 10.53, and 3.18 mg GAE per gram, respectively. DPPH radical scavenging activity showed slight variation. IC 50 of crude extract, DCM, EtOAc and Aq fractions were 11.13, 11.25, 11.74, and 11.28 µg mL -1 , respectively. Among the sample, ferrous ion chelating was the highest in crude extract (IC 50 302.90 µg mL -1 ), followed by EtOAc, Aq, and DCM fractions with 457.77, 547.91, and 641.82 µg mL -1 , respectively. Ferric-reducing antioxidant power showed optical density at about 0.5. The samples tested exhibited low hemolytic activity under 10% up to a concentration of 50 μg mL -1 . No antimicrobial activity was observed against any of the tested bacterial strains. For the cytotoxic activity, LC 50 of DCM, crude extract, EtOAc, and Aq were 52.10, 83.06, 86.34, and 117.45 μg mL -1 , showing high toxicity.

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“…Presence of a non-toxic lectin from Sebastiania jacobinensis bark to Artemia nauplii is also reported (Vaz et al, 2010). Even two lectins (Halilectin 1 and Halilectin 2) from the same source, a marine sponge, had been purified with quite different toxicities (6.4 and 142.1 μg/ml) (Carneiro et al, 2013). Comparing different LC 50 values of these lectins, it became evident that STL-S and STL-D exhibited an intermediary toxic effect against Artemia nauplii.…”

Section: Brine Shrimp Nauplii Lethality Assaymentioning

confidence: 84%

Antiproliferative activity of cytotoxic tuber lectins from Solanum tuberosum against experimentally induced Ehrlich ascites carcinoma in mice

Hasan¹,

Islam²,

Ozeki³

et al. 2014

Afr. J. Biotechnol.

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Cytotoxicity of tuber lectins from two potato cultivars was assessed and their anti-tumor potential against experimentally induced Ehrlich ascites carcinoma in Swiss albino mice was evaluated. Twenty (20) kDa chitin-binding lectins from Solanum tuberosum tubers, STL-S and STL-D were purified through ion-exchange and affinity chromatographic methods, hemagglutinating activity and blood group specificity of the lectins were checked whereas the cytotoxicity was determined using brine shrimp (Artemia salina L.) nauplii lethality assay. The lectins showed no specificity to animal and human erythrocytes. LC 50 values for STL-S and STL-D were found to be 75 and 90 μg/ml, respectively with a dose-dependent intermediary toxic effect. After inducing ascites by intraperitoneal propagation, the Swiss albino mice were treated by administering the lectins at a dose of 1.38 mg/kg/day for five consecutive days. STL-S and STL-D showed 79.84 and 83.04% of growth inhibition of EAC cells, respectively. Additionally, hemoglobin and RBC levels became considerably increased with a drop off in the WBC levels in the treated mice group indicating moderate anticancer activities exhibited by the potato lectins.

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Halilectin 1 (H‐1) and Halilectin 2 (H‐2): two new lectins isolated from the marine sponge Haliclona caerulea

Cited by 21 publications

References 37 publications

Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea

Hemagglutinating/Hemolytic activities in extracts of marine invertebrates from the Brazilian coast and isolation of two lectins from the marine sponge Cliona varians and the sea cucumber Holothuria grisea

Antioxidant, hemolytic, antimicrobial, and cytotoxic activities of the tropical Atlantic marine zoanthid Palythoa caribaeorum

Antiproliferative activity of cytotoxic tuber lectins from Solanum tuberosum against experimentally induced Ehrlich ascites carcinoma in mice

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