Antiproliferative activity of cytotoxic tuber lectins from Solanum tuberosum against experimentally induced Ehrlich ascites carcinoma in mice

Hasan, Imtiaj; Islam, Farhadul; Ozeki, Yasuhiro; Kabir, Syed Rashel

doi:10.5897/ajb2014.13633

Cited by 11 publications

(2 citation statements)

References 37 publications

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“…The hemagglutination assay of crude leaf and seed extracts of B. alba were performed in 96-well microtiter U-bottomed plates following the procedure described by Hasan I. et al, 2014 [37]. According to this process, goat blood was collected in test tubes containing sodium citrate (anticoagulant reagent) and washed for 2-3 times with PBS (phosphate buffer saline) to prepare 2% (w/v) red blood cells.…”

Section: Methodsmentioning

confidence: 99%

In VivoAnticancer Activity ofBasella albaLeaf and Seed Extracts against Ehrlich's Ascites Carcinoma (EAC) Cell Line

Islam

Rahi

Jahangir

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Cancer is a class of diseases characterized by uncontrolled cell growth. The current treatment options of cancer are radiotherapy, chemotherapy, hormone therapy, and surgery, where all of them have unpleasant side effects. Due to their adverse side effects, it is challenging to develop new drug for cancer treatment. Hence, the scientists are trying to seek for noble compounds from natural sources to treat cancer. Therefore, in the present investigation, a widely consumable vegetable Basella alba was subjected to evaluate its antiproliferative effect along with molecular signaling of apoptosis in Ehrlich ascites carcinoma (EAC) cell line. Cell growth inhibition was determined by haemocytometer whereas apoptosis of cancer cells were studied by florescence microscope using Hoechst-33342 stain and result was supported by DNA fragmentation and certain cancer related genes expression through PCR analysis. B. alba leaf and seed extract exhibit a considerable scavenging activity in comparison to a standard antioxidant BHT. Moreover, the leaf and seed extracts were able to agglutinate 2% RBC of goat blood at minimum 12.5μg/ml and 50.0μg/ml concentration, respectively. A significant cytotoxic activity was also found in both leaf and seed extract. In haemocytometic observation, the leaf and seed extracts exhibit about 62.54±2.41% and 53.96±2.34% cell growth inhibition, respectively, whereas standard anticancer drug Bleomycin showed 79.43±1.92% growth inhibition. Morphological alteration under fluorescence microscope showed nuclear condensation and fragmentation which is the sign of apoptosis. Apoptosis induction was also confirmed by DNA laddering in leaf and seed treated EAC cells. Upregulation of the tumor suppressor gene P53 and downregulation of antiapoptotic gene Bcl-2 enumerate apoptosis induction. Therefore, current study manifested that leaf and seed extracts of B. alba have antiproliferative activity against EAC cell line and can be a potent source of anticancer agents to treat cancer.

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Section: Methodsmentioning

confidence: 99%

In VivoAnticancer Activity ofBasella albaLeaf and Seed Extracts against Ehrlich's Ascites Carcinoma (EAC) Cell Line

Islam

Rahi

Jahangir

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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“…The hemagglutination assay of crude root and leaf extracts of J. dolomiaea were performed in 96-well micro-titer U-bottomed plates described by Hasan I. et al, [35]. According to this process, goat blood was collected in test tubes containing sodium citrate (anticoagulant reagent) and washed for 20 times with PBS (phosphate buffer saline) to prepare 2% (w/v) red blood cells.…”

Section: Hemagglutination Assaymentioning

confidence: 99%

In-vivo Anticancer Activity of Root and Leaf extract of Jurinea dolomiaea Boiss (Asteraceae) against Ehrlichs Ascites Carcinoma (EAC) Cell Line

Bhat¹,

Khan²,

Kataria³

2022

J. Adv. Chem. Sci.

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Cancer is a type of disease of uncontrolled growth cells. The currently available treatments are radiotherapy, chemotherapy, hormone therapy and surgery, in which all of them have side effects. Due to the adverse side effects, it is challenging to develop new drug for cancer treatment. Hence, the scientists are trying to seek the compounds from natural sources to treat cancer. Therefore, in this present investigation, root and leaf extracts of Jurinea dolomiaea Boiss has subjected to evaluate its anti-proliferative effect along with molecular signaling of apoptosis in Ehrlich ascites carcinoma (EAC) cell line. Jurinea dolomiaea root and leaf extracts exhibit a considerable scavenging activity in comparison to a standard antioxidant BHT. Moreover, root and leaf extracts were able to agglutinate 2% RBC of goat blood at minimum 12.5 g/mL and 50.0 g/mL concentration respectively. Cytotoxic activity was also found in root and leaf extract. In haemocytometic observation, the root and leaf extract exhibit about 62.54±2.41% and 53.96±2.34% cell growth inhibition respectively where as standard anticancer drug Bleomycin showed 79.43±1.92% growth inhibition. Morphological change under fluorescence microscope showed nuclear condensation and fragmentation which are the sign of apoptosis. Therefore, current results depict root extract of Jurinea dolomiaea Boiss have anti-proliferative activity against EAC cell line and can be a good source of anticancer agents to treat cancer.

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Solanum tuberosum lectin inhibits Ehrlich ascites carcinoma cells growth by inducing apoptosis and G2/M cell cycle arrest

Kabir

Amin

et al. 2016

Tumor Biol.

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Recently, a lectin was purified from the potato cultivated in Bangladesh locally known as Sheel. In the present study cytotoxicity of the lectin against Ehrlich ascites carcinoma (EAC) cells was studied by MTT assay in vitro in RPMI-1640 medium and 8.0-36.0 % cell growth inhibition was observed at the range of 2.5-160 μg/ml protein concentration when incubated for 24 h. The lectin-induced apoptosis in EAC cells was confirmed by fluorescence and optical microscope. The apoptotic cell death was also confirmed by using caspase inhibitors. Cells growth inhibition caused by the lectin (36 %) was remarkably decreased to 7.6 and 22.3 % respectively in the presence of caspase-3 and -8 inhibitors. RT-PCR was used to evaluate the expression of apoptosis-related genes Bcl-X, p53, and Bax. An intensive expression of Bcl-X gene was observed in untreated control EAC cells with the disappeared of the gene in Sheel-treated EAC cells. At the same time, Bax gene expression appeared only in Sheel-treated EAC cells and the expression level of the p53 gene was increased remarkable after the treatment of EAC cells with the lectin. The lectin showed strong agglutination activity against EAC cells. Flow cytometry was used to study the cell cycle phases of EAC cells and it was observed that the lectin arrested the G2/M phase. In conclusion, Sheel lectin inhibited EAC cells growth by inducing apoptosis.

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Antiproliferative activity of cytotoxic tuber lectins from Solanum tuberosum against experimentally induced Ehrlich ascites carcinoma in mice

Cited by 11 publications

References 37 publications

In VivoAnticancer Activity ofBasella albaLeaf and Seed Extracts against Ehrlich's Ascites Carcinoma (EAC) Cell Line

In VivoAnticancer Activity ofBasella albaLeaf and Seed Extracts against Ehrlich's Ascites Carcinoma (EAC) Cell Line

In-vivo Anticancer Activity of Root and Leaf extract of Jurinea dolomiaea Boiss (Asteraceae) against Ehrlichs Ascites Carcinoma (EAC) Cell Line

Solanum tuberosum lectin inhibits Ehrlich ascites carcinoma cells growth by inducing apoptosis and G2/M cell cycle arrest

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