Solanum tuberosum lectin inhibits Ehrlich ascites carcinoma cells growth by inducing apoptosis and G2/M cell cycle arrest

Kabir,; Mm, Rahman; Amin, Ruhul; Karim, Rezaul; Zh, Mahmud; Hossain, Mohammad Zabed

doi:10.1007/s13277-015-4735-x

Cited by 19 publications

(19 citation statements)

References 24 publications

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“…In vitro, exposure of EAC cells to various concentrations of MEHSR for 24 h triggered significant cell death in a dose dependent fashion ( Fig. 2a) and the results obtained from previous studies sustain the findings of this study [21], indicating the potentials of this plant extract as an anticancer agent.…”

Section: Discussionsupporting

confidence: 90%

“…Moreover, EAC cells treated with MEHSR showed characteristics features of apoptosis (cell membrane blebbing, nuclear condensation and fragmentation) whereas regular and round shape was found in EAC cells of untreated control. So characteristics of apoptotic death were observed in MEHSR treated EAC cells and similar type of observations were also reported by using different plant extracts [21,31]. All these are considered as imperative and promising characteristics of an antineoplastic agent [32].…”

Section: Discussionsupporting

confidence: 77%

“…MTT colorimetric assay was performed to measure the cytotoxicity of MEHSR against EAC cells [21]. In brief, EAC cells (2.5 × 10 5 in 200 μl RPMI 1640 media) along with MEHSR at different concentrations (15.625-500 μg/ml) were plated in each well of a culture plate (containing 96-well bottom), followed by incubation in CO 2 incubator at 37°C for 24 h. After the incubation period, aliquot was aspirated.…”

Section: In Vitro Cell Viability Test By Mtt Colorimetric Assaymentioning

confidence: 99%

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Antioxidant and antineoplastic activities of roots of Hibiscus sabdariffa Linn. Against Ehrlich ascites carcinoma cells

et al. 2019

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Background: The goal of this study was to explore the inherent antioxidant and antineoplastic activities of methanolic extract of the roots of Hibiscus sabdariffa (MEHSR). Methods: The dried coarse powder of roots of Hibiscus sabdariffa was subjected to methanolic extraction. Here in vitro methods were used to determine the various types of phytochemical content and antioxidant activity of MEHSR as well as its cytotoxic effect against Ehrlich ascites carcinoma (EAC) cells. In vivo, antineoplastic activity of MEHSR against EAC cells was also evaluated by determining the viable tumor cell count, survival time, body weight gain, hematological profiles of experimental mice along with observing morphological changes of EAC cells by fluorescence microscope. Analysis of the chemical composition of MEHSR was carried out using GC-MS. Results: Total phenolic and flavonoid contents of MEHSR were found to be 143.36 and 82.81 mg/g of extract in terms of gallic acid and catechin equivalent, respectively. The MEHSR exhibited very good scavenging property on DPPH (IC 50 : 13.37 μg/mL) and ABTS (IC 50 : 18.88 μg/mL) radicals in respect to nitric oxide (IC 50 : 72.82 μg/mL) radical and lipid peroxidation (IC 50 : 75.78 μg/mL) inhibition. MEHSR was found to induce Ehrlich ascites carcinoma (EAC) cell death at a dose dependent fashion. At dose 10 mg/kg, MEHSR significantly inhibited tumor cell growth rate (62.24%; p < 0.05), decreased tumor weight (57.81%; p < 0.05), increased life span (38.97%) compared to the untreated control mice. MEHSR also restored all hematological parameters of EAC-bearing mice towards normal level. Furthermore, administration of MEHSR induced apoptosis of EAC cells as observed in Hoechst 33342 stained cells under fluorescence microscope. Arachidic acid (49.18%), oleic acid (36.36%) and octadecanoic acid (14.47%) were identified as the major components of MEHSR by GC-MS analysis.Conclusion: In a nutshell, our findings proposed that MEHSR may possess promising antioxidant and antineoplastic efficacy against Ehrlich ascites carcinoma cells by induction of cell apoptosis. Therefore, it might be a potent and novel candidate for anticancer therapy.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 77%

Section: In Vitro Cell Viability Test By Mtt Colorimetric Assaymentioning

confidence: 99%

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Antioxidant and antineoplastic activities of roots of Hibiscus sabdariffa Linn. Against Ehrlich ascites carcinoma cells

et al. 2019

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“…Based on the literature, it is possible that the cytotoxicity observed in our experiments is due to apoptosis. Lectins isolated from different plants increase expression of Bax (pro-apoptotic molecule) and is dependent of caspase 3, 8, and 9 (Kabir et al, 2013, 2016; Kumar et al, 2014). It is interesting to note that lectins induce ROS production and these species is important to trigger the apoptosis mechanisms (da Mota et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Eutirucallin: A Lectin with Antitumor and Antimicrobial Properties

Palharini

Richter

Silva

et al. 2017

Front. Cell. Infect. Microbiol.

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Eutirucallin is a lectin isolated from the latex of Euphorbia tirucalli, a plant known for its medical properties. The present study explores various characteristics of Eutirucallin including stability, cytotoxicity against tumor cells, antimicrobial and antiparasitic activities. Eutirucallin was stable from 2 to 40 days at 4°C, maintained hemagglutinating activity within a restricted range, and showed optimal activity at pH 7.0–8.0. Eutirucallin presented antiproliferative activity for HeLa, PC3, MDA-MB-231, and MCF-7 tumor cells but was not cytotoxic for non-tumorigenic cells such as macrophages and fibroblasts. Eutirucallin inhibited the Ehrlich ascites carcinoma in vivo and it was also observed that Eutirucallin inhibited 62.5% of Escherichia coli growth. Also, Eutirucallin showed to be effective when tested directly against Toxoplasma gondii infection in vitro. Therefore, this study sheds perspectives for pharmacological applications of Eutirucallin.

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“…Further analysis of mitochondrial pathway of apoptosis revealed that VC-III treatment decreased the expression of prosurvival proteins Bcl-2 and moderately increased the expression levels of proapoptotic Bcl-2 family protein, Bax. 36 Thus, VC-III induced release of cyt c into the cytosol. Studies have shown that caspase activation acts as the downstream of cyt c release during mitochondrial pathway of apoptosis.…”

Section: Vc-iii Activated P53 and Intensified Intrinsic Apoptotic Patmentioning

confidence: 96%

Vanadium(III)-l-cysteine enhances the sensitivity of murine breast adenocarcinoma cells to cyclophosphamide by promoting apoptosis and blocking angiogenesis

et al. 2017

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Various epidemiological and preclinical studies have already established the cancer chemopreventive potential of vanadiumbased compounds. In addition to its preventive efficacy, studies have also indicated the abilities of vanadium-based compounds to induce cell death selectively toward malignant cells. Therefore, the objective of the present investigation is to improve the therapeutic efficacy and toxicity profile of an alkylating agent, cyclophosphamide, by the concurrent use of an organovanadium complex, vanadium(III)-l-cysteine. In this study, vanadium(III)-l-cysteine (1 mg/kg body weight, per os) was administered alone as well as in combination with cyclophosphamide (25 mg/kg body weight, intraperitoneal) in concomitant and pretreatment schedule in mice bearing breast adenocarcinoma cells. The results showed that the combination treatment significantly decreased the tumor burden and enhanced survivability of tumor-bearing mice through generation of reactive oxygen species in tumor cells. These ultimately led to DNA damage, depolarization of mitochondrial membrane potential, and apoptosis in tumor cells. Further insight into the molecular pathway disclosed that the combination treatment caused upregulation of p53 and Bax and suppression of Bcl-2 followed by the activation of caspase cascade and poly (ADP-ribose) polymerase cleavage. Administration of vanadium(III)-l-cysteine also resulted in significant attenuation of peritoneal vasculature and sprouting of the blood vessels by decreasing the levels of vascular endothelial growth factor A and matrix metalloproteinase 9 in the ascites fluid of tumor-bearing mice. Furthermore, vanadium(III)-l-cysteine significantly attenuated cyclophosphamide-induced hematopoietic, hepatic, and genetic damages and provided additional survival advantages. Hence, this study suggested that vanadium(III)-l-cysteine may offer potential therapeutic benefit in combination with cyclophosphamide by augmenting anticancer efficacy and diminishing toxicity to the host.

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Solanum tuberosum lectin inhibits Ehrlich ascites carcinoma cells growth by inducing apoptosis and G2/M cell cycle arrest

Cited by 19 publications

References 24 publications

Antioxidant and antineoplastic activities of roots of Hibiscus sabdariffa Linn. Against Ehrlich ascites carcinoma cells

Antioxidant and antineoplastic activities of roots of Hibiscus sabdariffa Linn. Against Ehrlich ascites carcinoma cells

Eutirucallin: A Lectin with Antitumor and Antimicrobial Properties

Vanadium(III)-l-cysteine enhances the sensitivity of murine breast adenocarcinoma cells to cyclophosphamide by promoting apoptosis and blocking angiogenesis

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