Haemorrhagic fever with renal syndrome: evaluation of ELISA for detection of Puumala-virus-specific IgG and IgM

Prevalence of antibodies specific to Puumala virus among farmers in Sweden

“…First, the sensitivity of the recombinant protein based ELISA is high (1 4). Moreover, antibodies to Puumala virus endure for decades after the infection is contracted (27,28); therefore serological investigation should have detected most of the persons affected irrespective of time lapse after infection. Finally, we selected a group of subjects shown to be at high risk of infection.…”

Hemorrhagic fever with renal syndrome: a study of sequelae following Nephropathia epidemica

“…The study in Norsj6 took advantage of the "Norsj6 project" giving an unique opportunity to investigate a very well defined population living in the most HFRS endemic area of Sweden. Puumala virus infection gives a serological response characterized by high titers of specific IgG for more than 10-20 years, probably life-long [21,24]. The parameters included in the "Norsj6 project" were selected focusing on cardiovascular diseases.…”

“…All clinical cases of NE were confirmed using a PUU virus specific g-capture IgM ELISA as described in detail in previous publications [20,21]. Briefly, goat anti human IgM was adsorbed to the wells, test sera diluted t :400 were added, followed by PUU virus antigen (antigen was prepared from PUU virus (strain Sotkamo) infected Vero E-6 cells), rabbit anti-PUU virus Ig, alkaline phosphatase-labelled goat anti-rabbit IgG and p-nitrophenyl phosphatase substrate.…”

“…Screening of immunity to NE as a sign of previous infection in a normal population was measured by detection of PUU virus specific IgG antibodies by ELISA. The IgG ELISA has also been described in detail in a previous publication [21]. In short, a sandwich ELISA was employed as follows.…”

The Hantaviruses, Etiologic Agents of Hemorrhagic Fever with Renal Syndrome: A Possible Cause of Hypertension and Chronic Renal Disease in the United States

“…Standard serological tests to confirm a diagnosis of HFRS are mostly based on immunofluorescent or enzyme-linked im munosorbent assays to detect immunoglobulin G (29, 64,67,69,90). However, for epidemiological monitoring in regions where more than one hantavirus circulates, positive identification of the infecting agent may de pend on cumbersome cross-plaque-reduction neutralization assays of serum performed on batteries of hantaviruses under laboratory containment con ditions (91).…”