Haemato-biochemical responses and induction of HSP70 to dietary phosphorus in Catla catla (Hamilton) fingerlings

Abstract

A feeding trial of 120 days was conducted to study the effect of graded levels of dietary phosphorus on haematology, serum protein concentrations and HSP70 expression in fingerlings of the Indian major carp, Catla (Catla catla). Eight isonitrogenous and isoenergetic purified diets were formulated to contain graded levels of dietary phosphorus (dP), i.e., T(1), 0.1%; T(2), 0.3%; T(3), 0.5%; T(4), 0.7%; T(5), 0.9%; T(6), 1.1%; T(7), 1.3%; or T(8), 1.5%. Four hundred and eighty fish (average weight 4.23 +/- 0.016 g) were equally distributed into 24 tanks forming eight treatments with three replicates each. The fish were fed daily at the rate of 3.5% body weight in two instalments. At the end of feeding trial fish were sampled to study total RBC and WBC count, haemoglobin, serum lysozyme activity, serum total protein, albumin (A), globulin (G) concentration and HSP70 expression. Total RBC count, haemoglobin concentration and serum lysozyme activity did not vary significantly in response to different dietary phosphorus concentrations. Total WBC count was found to be significantly (P < 0.05) higher in T(1 )relative to all other treatments. Serum albumin and A/G ratio was found to be significantly lower in fish of T(1) and T(2) in relation to T(7) group (P < 0.05). Serum globulin and total protein levels remained unaffected by variations in dietary phosphorus. HSP70 expression was observed in T(1) group (0.1% dP) in gills and brain tissue, but not in liver and muscle tissues. No HSP70 expression was observed in fish of T(4) (0.7% dP) and T(8) (1.5% dP) treatments. These prima facie results suggest that dietary phosphorus had only minor influence on the haemato-biochemical parameters studied; however dietary phosphorus deficiency caused organ specific induction of HSP70 in catla fingerlings.

Dietary available phosphorus requirement for tambaqui,Colossoma macropomum, juveniles based on growth, haematology and bone mineralization

“…P supplementation only affected total leucocyte count of Indian major carp ( Catla catla ) (Sukumaran et al . ). However, except for haematometric indices, all other haematological parameters were affected by P supplementation in our study.…”

“…), and this last parameter is regarded as an essential factor for the development of aquaculture (Sukumaran et al . ).…”

“…Similarly, inconclusive effects of dietary P supplementation on haematology and blood chemistry have been reported for Indian major carp, Catla catla (Sukumaran et al . ).…”

Effect of phosphorus deficiency on erythrocytic morphology and function in cows

“…P deficiency decreases the life span of erythrocytes, an effect presumably resulting from energy depletion. ATP has a significant role in the functioning of a cell [ 28 ]. ATP is considered by biologists to be the energy currency of life, as ATP transports chemical energy within cells for metabolic purposes [ 25 ].…”

Advances and perspectives on the regulation and expression of piscine heat shock proteins

“…Indeed such approaches have been used recently in fish and have yielded valuable data (Geist et al 2007;Williams et al 2008). Due consideration should also be given to the possible effects of nutritional status on the fish HSP response as conditions of starvation (Zarate and Bradley 2003;Cara et al 2005;Weber and Bosworth 2005;Yengkokpam et al 2008) or suboptimal diet (Martin et al 2003;Sukumaran et al 2008) alter HSP expression during stress. We also need to address, from a mechanistic perspective, if and how HSPs play a major protective role in fish.…”