Hadamard transform capillary electrophoresis combined with laser-induced fluorometry using electrokinetic injection

Hata, Kazuki; Kaneta, Takashi; Imasaka, Totaro

doi:10.1016/j.aca.2005.06.068

Cited by 18 publications

(15 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hata et al [93] presented the application of Hadamard transform technique, a multiplexing method, to CE for AA applications, using a particular experimental CE setup. This technique theoretically improves the S/N ratio by a factor of (n 1 1)/2n 1/2 , where n is the order of the Hadamard matrix, i.e., the number of injections used for the Hadamard transformation [94].…”

Section: Discussionmentioning

confidence: 99%

Recent advances in amino acid analysis by CE

et al. 2007

View full text Add to dashboard Cite

This paper describes a number of articles that have been published on amino acid (AA) analysis using CE during the period from June 2005 to May 2007. This review article follows the format of the previous articles of Smith (Electrophoresis 1999, 20, 3078-3083), Prata et al.. (Electrophoresis 2001, 22, 4129-4138), and Poinsot et al.. (Electrophoresis 2003, 24, 4047-4062 and Electrophoresis 2006, 27, 176-194). Several new developments in AA analysis with CE are reported describing the use of laser-emitting diodes for LIF, MS, and chips. In addition, we describe articles concerning clinical studies and neuroclinical applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Recent advances in amino acid analysis by CE

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The discrepancy can be attributed to the number of sample components and/or instability associated with EOF. We previously reported that the improvement factor obtained by a Hadamard transformation was slightly reduced in the case of a multiple component analysis [9,12,13]. Moreover, the addition of high concentrations of SDS leads to Joule heating, which may degrade the reproducibility in EOF.…”

Section: Application To the Separation Of Amino Acidsmentioning

confidence: 96%

“…The data obtained by multiple sample injection were then mathematically transformed into data for which the S/N is remarkably increased. In our previous study, the application of a Hadamard transform (HT) technique to CZE was demonstrated, in which substantial improvement in sensitivity was accomplished, as predicted from theory [6][7][8][9][10][11][12][13]. In this technique, multiple sample injection according to a Hadamard code sequence derived from a Hadamard matrix was employed for CZE separation.…”

Section: Introductionmentioning

confidence: 96%

Application of Hadamard transformation to MEKC

2007

Self Cite

View full text Add to dashboard Cite

The Hadamard transform (HT) technique, which permits the S/N in CE to be improved, was applied to MEKC. Multiple sample injection of fluorescent analytes according to a Hadamard code sequence was performed using an optically gated sample injection technique, in which a sample plug was produced based on photodegradation by irradiation with an intense laser beam. The capillary and reservoirs were filled with a sample solution containing buffer components and SDS as a pseudostationary phase. A preliminary study confirmed that fluorescein ion could be photobleached in the presence of SDS. The optically gated sample injection technique was then applied to multiple sample injection, based on a Hadamard matrix. The S/N in the electropherogram obtained by HT-MEKC was improved substantially compared to that obtained by a single injection method. When the technique was applied to the separation of several amino acids labeled with FITC, the S/N ratio for each amino acid was enhanced, without any evidence of degradation in separation resolution. Moreover, HT-MEKC was applied to the analysis of amino acids contained in a Japanese beverage, resulting in improved S/Ns for the amino acids.

show abstract

“…Accordingly, the SNR of IMS has been improved nearly 2-10 folds. [1][2][3] Due to that, the Hadamard multiplexing has been efficiently introduced into many other fields, such as the capillary electrophoretic separation technique (CE), [4][5][6][7] time-of-flight mass spectrometry, [8][9][10] gas chromatography/ mass spectrometry (GC/MS) and so on. [11][12][13] However, with the enhancement of the SNR, the artifacts were unexpectedly found in the demultiplexed data of HT techniques.…”

Section: Introductionmentioning

confidence: 99%

“…14 Zeppenfeld et al analyzed that the negative systematic false peak errors were introduced by the instrumentation in the analysis of time of flight mass spectrometry. 15 Besides, the false or spurious peaks were also located in data of HT-CE, 6,7 Time-of-flight Mass Spectrometry. 16 Recently, our group focuses on the study of the artifacts in HT-IMS and have put forward some efficient methods to suppress these false peak artifacts.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Baseline Drift Artifact in Hadamard Transform Separation Techniques

Yan¹,

Su²,

Tang³

et al. 2019

Quím. Nova

View full text Add to dashboard Cite

The baseline drift artifact (BDA), namely, the baseline bias between the Hadamard transform (HT) spectrum and the signal averaging (SA) spectrum was unfortunately found in the HT pulsed separation techniques, which makes the HT analytical chemistry face the challenge of non-stability and unreliability. In order to find out the origin and eliminate the BDA phenomenon, the multiplexing mechanism of Hadamard transform ion mobility spectrometry (HT-IMS) has been analyzed in this research. Through simulation and experimental comparisons, the non-ideal overlap defect in Hadamard multiplexing process was suggested as the cause for the BDA phenomenon. Eventually, with a correction method, the BDA phenomenon could be well addressed, which guarantees the reliability and stability of HT techniques without increasing the hardware complexity.

show abstract

Hadamard transform capillary electrophoresis combined with laser-induced fluorometry using electrokinetic injection

Cited by 18 publications

References 20 publications

Recent advances in amino acid analysis by CE

Recent advances in amino acid analysis by CE

Application of Hadamard transformation to MEKC

Analysis of the Baseline Drift Artifact in Hadamard Transform Separation Techniques

Contact Info

Product

Resources

About