Gut region influences the diversity and interactions of bacterial communities in pikas (Ochotona curzoniae and Ochotona daurica)

Li, Huan; Li, Tongtong; Berasategui, Aileen; Rui, Junpeng; Zhang, Xiao; Li, Chaonan; Xiao, Zhishu; Li, Xiangzhen

doi:10.1093/femsec/fix149

Cited by 22 publications

(29 citation statements)

References 55 publications

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“…In addition, functional changes have also been reported between cecal and fecal microbiota in mouse (Tanca et al., ). Our study further confirms that fecal samples, although easily accessible, cannot be used as a proxy of the microbiota in other gut regions (Li, Li, et al., b).…”

Section: Discussionsupporting

confidence: 87%

“…However, recent studies have shown that microbiota community compositions varied between different gut regions and feces of mice, (Gu et al., ; Looft et al., ; Mu et al., ) and pikas (Li, Chen, et al., ) possibly because microbiotas play different functional roles in different intestinal compartments and niches. To date, very few such comparisons have been conducted in wild animals (but see Kohl et al., ; Li, Li, et al., ) to test whether fecal samples can be used as a proxy of the microbiota in other gut regions.…”

Section: Introductionmentioning

confidence: 99%

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Gut microbial diversity in two insectivorous bats: Insights into the effect of different sampling sources

Xing

Sun

et al. 2018

MicrobiologyOpen

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The gut microbiota is now known as a key factor in mammalian physiology and health. Our understanding of the gut microbial communities and their effects on ecology and evolution of their hosts is extremely limited in bats which represent the second largest mammalian order. In the current study, gut microbiota of three sampling sources (small intestine, large intestine, and feces) were characterized in two sympatric and insectivorous bats (Rhinolophus sinicus and Myotis altarium) by high-throughput sequencing of the V3-V4 region of the 16S rRNA gene. Combining with published studies, this work reveals that Gammaproteobacteria may be a dominant class in the whole Chiroptera and Fusobacteria is less observed in bats although it has been proven to be dominant in other mammals. Our results reveal that the sampling source influences alpha diversity of the microbial community in both studied species although no significant variations of beta diversity were observed, which support that fecal samples cannot be used as a proxy of the microbiota in other gut regions in wild animals.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Gut microbial diversity in two insectivorous bats: Insights into the effect of different sampling sources

Xing

Sun

et al. 2018

MicrobiologyOpen

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“…For example, species from Streptococcus are generally common bacterial taxa in human skin, mouth, throat and intestine (van der Mee‐Marquet et al ., ; Zaura et al ., ; Chaffanel et al ., ). Our previous study also showed that Streptococcus was one of the dominant bacterial taxa in pika mouths (Li et al ., ), indicating that this genus probably widely distributes in mammalian microbiota. Since some species in this genus have been reported to be involved in pyruvate metabolism in humans (Jorth et al ., ), we speculate they play important roles in the gut niche of high‐altitude pikas.…”

Section: Discussionmentioning

confidence: 97%

“…Our previous studies and some other reports on pika gut microbiota suggested that host (e.g. genetics, population density, gut regions) (Li et al ., , 2017a,b) and environmental factors (e.g. diet, environmental microbes) (Li et al ., 2016b,d; Kohl et al ., ) considerably influence the gut community composition and diversity patterns.…”

Section: Introductionmentioning

confidence: 99%

Environmental filtering increases with elevation for the assembly of gut microbiota in wild pikas

Zhou

Zhu

et al. 2019

Microbial Biotechnology

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Summary Despite their important roles in host nutrition, metabolism and adaptability, the knowledge on how the mammalian gut microbial community assemble is relatively scanty, especially regarding the ecological mechanisms that govern microbiota along environmental gradients. To address this, we surveyed the diversity, function and ecological processes of gut microbiota in the wild plateau pika, Ochotona curzoniae, along the elevational gradient from 3106 to 4331 m on ‘the Roof of the World’—Qinghai‐Tibet Plateau. The results indicated that the alpha, beta and functional diversity of gut microbiota significantly increased with elevation, and elevation significantly explained the variations in the gut microbial communities, even after controlling for geographical distance, host sex and body weight. Some gene functions (e.g. nitrogen metabolism and protein kinases) associated with metabolism were enriched in the high‐altitude pikas. Null model and phylogenetic analysis suggest that the relative contributions of environmental filtering responsible for local gut communities increased with elevation. In addition, deterministic processes dominated gut microbial communities in the high‐altitude (more than 3694 m) pikas, while the percentages of stochastic and deterministic processes were very close in the low‐altitude (3106 and 3580 m) pikas. The observed mechanisms that influence pika gut microbiota assembly and function seemed to be mainly mediated by the internal gut environment and by the external environmental pressure (i.e. lower temperature) in the harsh high‐altitude environment. These findings enhance our understanding of gut microbiota assembly patterns and function in wild mammals from extreme harsh environments.

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“…An analysis of similarity ( anosim ) was used to reveal whether the beta diversity was significantly different across the pregnancy and parturition stages based on the weighted UniFrac matrices using the ‘vegan’ package in the R program (Li et al ). The effects of the different stages on backfat thickness, plasma biochemical parameters, the apparent relative abundances of communities at the phylum and genus level and the alpha diversity indices were analysed using a one‐way analysis of variance with LSD post hoc test in SAS (SAS Institute, Inc., Cary, NC).…”

Section: Methodsmentioning

confidence: 99%

Stages of pregnancy and weaning influence the gut microbiota diversity and function in sows

Xie

et al. 2019

J Appl Microbiol

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AimsThe gut microbiota is believed to play important roles in the health of pregnant mammals, including their nutrient metabolism, immune programming and metabolic regulation. However, until recently, the shifts in gut microbiota composition and faecal and blood metabolic activity during different stages of pregnancy had not been investigated.Methods and ResultsWe investigated the shifts in backfat thickness, plasma and faecal metabolites and gut microbiota on days 30, 60, 90 and 110 of pregnancy and on day 21 after parturition (weaning) in sows. The backfat thickness of sows did not significantly differ among the different stages of pregnancy. The plasma concentrations of lipid metabolites, including triacylglycerol (TG), total cholesterol, high‐density lipoprotein‐cholesterol, low‐density lipoprotein‐cholesterol and calcium were reduced (P < 0·05) during pregnancy. In addition, the concentration of these metabolites, except TG, reached their maximum at the time of weaning. We also found that Tenericutes, Fibrobacteres and Cyanobacteria varied significantly according to the stages of pregnancy in sows (P < 0·05). Most of the genera, such as Clostridiales, Desulfovibrio, Mogibacteriaceae and Prevotella, increased (P < 0·05) with the progression of pregnancy and decreased (P < 0·05) at weaning. The alpha diversity values (i.e., Shannon diversity and observed species) of sow gut microbiota increased (P < 0·05) from pregnancy to weaning. Pregnancy stages also significantly influenced (P < 0·05) the community structure (beta diversity) of gut microbiota. The progression of pregnancy was associated with changes in lipid metabolism and several carbohydrate‐degradation bacteria (i.e., Prevotella, Succinivibrio, Bacteroides and Parabacteroides).ConclusionsAlthough causal links between the measured parameters remain hypothetical, these findings suggest that the increased diversity and concentration of beneficial gut microbes are associated with the metabolism of pregnant sows.Significance and Impact of the StudyManipulation of the sow gut microbiota composition may potentially influence metabolism and health during pregnancy.

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Gut region influences the diversity and interactions of bacterial communities in pikas (Ochotona curzoniae and Ochotona daurica)

Cited by 22 publications

References 55 publications

Gut microbial diversity in two insectivorous bats: Insights into the effect of different sampling sources

Gut microbial diversity in two insectivorous bats: Insights into the effect of different sampling sources

Environmental filtering increases with elevation for the assembly of gut microbiota in wild pikas

Stages of pregnancy and weaning influence the gut microbiota diversity and function in sows

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