AimsThe gut microbiota is believed to play important roles in the health of pregnant mammals, including their nutrient metabolism, immune programming and metabolic regulation. However, until recently, the shifts in gut microbiota composition and faecal and blood metabolic activity during different stages of pregnancy had not been investigated.Methods and ResultsWe investigated the shifts in backfat thickness, plasma and faecal metabolites and gut microbiota on days 30, 60, 90 and 110 of pregnancy and on day 21 after parturition (weaning) in sows. The backfat thickness of sows did not significantly differ among the different stages of pregnancy. The plasma concentrations of lipid metabolites, including triacylglycerol (TG), total cholesterol, high‐density lipoprotein‐cholesterol, low‐density lipoprotein‐cholesterol and calcium were reduced (P < 0·05) during pregnancy. In addition, the concentration of these metabolites, except TG, reached their maximum at the time of weaning. We also found that Tenericutes, Fibrobacteres and Cyanobacteria varied significantly according to the stages of pregnancy in sows (P < 0·05). Most of the genera, such as Clostridiales, Desulfovibrio, Mogibacteriaceae and Prevotella, increased (P < 0·05) with the progression of pregnancy and decreased (P < 0·05) at weaning. The alpha diversity values (i.e., Shannon diversity and observed species) of sow gut microbiota increased (P < 0·05) from pregnancy to weaning. Pregnancy stages also significantly influenced (P < 0·05) the community structure (beta diversity) of gut microbiota. The progression of pregnancy was associated with changes in lipid metabolism and several carbohydrate‐degradation bacteria (i.e., Prevotella, Succinivibrio, Bacteroides and Parabacteroides).ConclusionsAlthough causal links between the measured parameters remain hypothetical, these findings suggest that the increased diversity and concentration of beneficial gut microbes are associated with the metabolism of pregnant sows.Significance and Impact of the StudyManipulation of the sow gut microbiota composition may potentially influence metabolism and health during pregnancy.

Intercontinental exchanges between communities living in different parts of Eurasia during the late prehistoric period have become increasingly popular as a topic of archaeological research. The Qijia culture, found in northwest China, is one of the key archaeological cultures that can shed light on trans-Eurasian exchange because a variety of imports are found in this cultural context. These imports include new cereals and animals, which suggest that human diets may also have changed compared with previous periods. To understand human and animal diets of the Qijia culture, carbon and nitrogen isotope ratios from human and animal skeletal remains were analysed from the type site of the Qijia culture at Qijiaping. The results demonstrate that human diet at the site mainly consisted of millet and animals fed on millet. C 3 cereals, such as wheat and barley, did not contribute significantly to human diet, and no isotopic differences were found between adult and subadult diets. Furthermore, three outlying human results raise the possibility of exogenous individuals, perhaps in relation to the parallel movement of animals, crops and goods. This study provides human and animal dietary information for evaluating the nature of exchange and diffusion in eastern Eurasia at this time.

No abstract

No abstract

No abstract