Overexpression of Small Heat Shock Protein LimHSP16.45 in Arabidopsis hsp17.6II Mutant Enhances Tolerance to Abiotic Stresses

Yang, Rutong; Yu, Guanzhong; Li, H.; Li, X.; Mu, Cuicui

doi:10.1134/s102144372002017x

Cited by 18 publications

(6 citation statements)

References 30 publications

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“…HSP20s accumulation is essential for basal thermotolerance and the onset of AT in plants. In A. thaliana , an AtHSP17.6II mutant was unable to establish the AT, whereas the overexpression of LimHSP16.45 from Lilium davidii rescued the sensibility to HS ( Yang et al, 2020 ). Supporting these observations, A. thaliana RNAi lines of six cytosolic HSP20s showed higher thermosensitivity, whereas the HSP20s overexpression lines restored the phenotype ( McLoughlin et al, 2016 ).…”

Section: The Role Of Small Heat Shock Proteins Under Heat Stressmentioning

confidence: 99%

Heat Shock Signaling in Land Plants: From Plasma Membrane Sensing to the Transcription of Small Heat Shock Proteins

Bourgine

Guihur

2021

Front. Plant Sci.

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Heat stress events are major factors limiting crop productivity. During summer days, land plants must anticipate in a timely manner upcoming mild and severe temperature. They respond by accumulating protective heat-shock proteins (HSPs), conferring acquired thermotolerance. All organisms synthetize HSPs; many of which are members of the conserved chaperones families. This review describes recent advances in plant temperature sensing, signaling, and response. We highlight the pathway from heat perception by the plasma membrane through calcium channels, such as cyclic nucleotide-gated channels, to the activation of the heat-shock transcription factors (HSFs). An unclear cellular signal activates HSFs, which act as essential regulators. In particular, the HSFA subfamily can bind heat shock elements in HSP promoters and could mediate the dissociation of bound histones, leading to HSPs transcription. Although plants can modulate their transcriptome, proteome, and metabolome to protect the cellular machinery, HSP chaperones prevent, use, and revert the formation of misfolded proteins, thereby avoiding heat-induced cell death. Remarkably, the HSP20 family is mostly tightly repressed at low temperature, suggesting that a costly mechanism can become detrimental under unnecessary conditions. Here, the role of HSP20s in response to HS and their possible deleterious expression at non-HS temperatures is discussed.

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Section: The Role Of Small Heat Shock Proteins Under Heat Stressmentioning

confidence: 99%

Heat Shock Signaling in Land Plants: From Plasma Membrane Sensing to the Transcription of Small Heat Shock Proteins

Bourgine

Guihur

2021

Front. Plant Sci.

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“…In this study, when cut lily flowers were treated with high temperature, low temperature, GABA and ABA, LbHSP17.9 transcription was induced; when LbHSP17.9 was silenced in cut lily flowers, the MDA content was higher than in TRV2; and when LbHSP17.9 was overexpressed in lily petal segments, the OE-LbHSP17.9 segments were brighter in color and had lower relative conductivity than the control under 4 • C stress. The gene LimHSP16.45 from Lilium davidii was cloned into A. thaliana and improved plant resistance to high temperature, high salt and oxidative stresses [23]. In this study, the leaves of two overexpressing A. thaliana lines under 4 • C treatment were greener than those of the WT, and the MDA content in the leaves was also significantly lower than that of the WT, indicating that overexpressing LbHSP17.9 improved the ability of A. thaliana to withstand cold stress.…”

Section: Discussionmentioning

confidence: 99%

LbHSP17.9 Participated in the Regulation of Cold Stress in Cut Lily Flowers

Zhao,

Yan,

Huang

et al. 2024

Horticulturae

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Heat shock proteins (HSPs) play important roles in plant stress resistance, but it is not clear whether small molecular HSPs (sHSPs) are involved in the cold stress resistance of lily flowers. In this study, we cloned LbHSP17.9 and found that its expression was up-regulated under cold stress. When LbHSP17.9 was silenced (TRV2::LbHSP17.9) using virus-induced gene silencing in cut lily flowers, the content of malondialdehyde was increased under 4 °C stress treatment. The catalase (CAT) activity in TRV2::LbHSP17.9 was significantly lower than in TRV2 in the first 7 days, and the peroxidase (POD) activity in TRV2::LbHSP17.9 was significantly lower than in TRV2 after 4 days of 4 °C stress. Further analysis showed that the transcription levels of LbCu/ZnSOD, LbMnSOD and LbCAT in TRV2::LbHSP17.9 were lower than those of TRV2 under 4 °C stress. When LbHSP17.9 was overexpressed in lily petal disks, the OE-LbHSP17.9 disks faded later than the controls at 4 °C and the relative conductivity decreased significantly. Overexpression of LbHSP17.9 in Arabidopsis thaliana resulted in fewer injury symptoms and lower MDA content than wild type under 4 °C stress. Therefore, we speculate that LbHSP17.9 can improve the resistance of lily flowers to cold stress.

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“…Heat stress upregulated most HSR genes at the transcriptional level, which usually had opposite expression patterns under standard growth conditions. The overexpression of many of these HSR genes, such as DDF1 [ 27 ], ATHSFA2 [ 28 ], AT-HSFB2A [ 29 ], BIP3 [ 30 ], MIPS2 [ 31 ], HSP17.6II [ 32 ], HSP17.6B-CI [ 33 ], Hsp70b [ 34 ], HSP70 [ 35 ], and CYP71B2 [ 36 ], would facilitate improved tolerance to heat stress. Thus, we conclude that the overexpression of HSR genes during heat stress may contribute to BrPP5.2-mediated heat stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

BrPP5.2 Overexpression Confers Heat Shock Tolerance in Transgenic Brassica rapa through Inherent Chaperone Activity, Induced Glucosinolate Biosynthesis, and Differential Regulation of Abiotic Stress Response Genes

Muthusamy

Kim

et al. 2021

IJMS

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Plant phosphoprotein phosphatases are ubiquitous and multifarious enzymes that respond to developmental requirements and stress signals through reversible dephosphorylation of target proteins. In this study, we investigated the hitherto unknown functions of Brassica rapa protein phosphatase 5.2 (BrPP5.2) by transgenic overexpression of B. rapa lines. The overexpression of BrPP5.2 in transgenic lines conferred heat shock tolerance in 65–89% of the young transgenic seedlings exposed to 46 °C for 25 min. The examination of purified recombinant BrPP5.2 at different molar ratios efficiently prevented the thermal aggregation of malate dehydrogenase at 42 °C, thus suggesting that BrPP5.2 has inherent chaperone activities. The transcriptomic dynamics of transgenic lines, as determined using RNA-seq, revealed that 997 and 1206 (FDR < 0.05, logFC ≥ 2) genes were up- and down-regulated, as compared to non-transgenic controls. Statistical enrichment analyses revealed abiotic stress response genes, including heat stress response (HSR), showed reduced expression in transgenic lines under optimal growth conditions. However, most of the HSR DEGs were upregulated under high temperature stress (37 °C/1 h) conditions. In addition, the glucosinolate biosynthesis gene expression and total glucosinolate content increased in the transgenic lines. These findings provide a new avenue related to BrPP5.2 downstream genes and their crucial metabolic and heat stress responses in plants.

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Overexpression of Small Heat Shock Protein LimHSP16.45 in Arabidopsis hsp17.6II Mutant Enhances Tolerance to Abiotic Stresses

Cited by 18 publications

References 30 publications

Heat Shock Signaling in Land Plants: From Plasma Membrane Sensing to the Transcription of Small Heat Shock Proteins

Heat Shock Signaling in Land Plants: From Plasma Membrane Sensing to the Transcription of Small Heat Shock Proteins

LbHSP17.9 Participated in the Regulation of Cold Stress in Cut Lily Flowers

BrPP5.2 Overexpression Confers Heat Shock Tolerance in Transgenic Brassica rapa through Inherent Chaperone Activity, Induced Glucosinolate Biosynthesis, and Differential Regulation of Abiotic Stress Response Genes

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