Gut-on-Chip microphysiological systems: Latest advances in the integration of sensing strategies and adoption of mature detection mechanisms

Signore, M.A.; Pascali, Chiara De; Giampetruzzi, Lucia; Siciliano, P.; Francioso, Luca

doi:10.1016/j.sbsr.2021.100443

Cited by 25 publications

(18 citation statements)

References 168 publications

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“…Of course, the length of exposure of the microbiome to the potential perturbator would be a critical consideration. Overall, however, this tier could be a reasonable approach to screen MDCs based on the resilience of the GM under various experimental approaches, extending from “humanized” animals ( National Academies of Sciences, Engineering, and Medicine [NASEM], 2018 ) to ex vivo and in vitro models, such as simulator of the human intestinal microbial ecosystem (SHIME) ( Van den Abbeele et al, 2012 ), minibioreactor arrays ( Auchtung et al, 2015 ) and multi-compartment microfluidic-based gut-on-chip systems ( De Gregorio et al, 2020 ; Signore et al, 2021 ).…”

Section: Incorporating the Gut Microbiome In Food Safety Risk Assessm...mentioning

confidence: 99%

Incorporating the Gut Microbiome in the Risk Assessment of Xenobiotics and Identifying Beneficial Components for One Health

et al. 2022

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Three areas of relevance to the gut microbiome in the context of One Health were explored; the incorporation of the microbiome in food safety risk assessment of xenobiotics; the identification and application of beneficial microbial components to various areas under One Health, and; specifically, in the context of antimicrobial resistance. Although challenging, focusing on the microbiota resilience, function and active components is critical for advancing the incorporation of microbiome data in the risk assessment of xenobiotics. Moreover, the human microbiota may be a promising source of beneficial components, with the potential to metabolize xenobiotics. These may have possible applications in several areas, e.g., in animals or plants for detoxification or in the environment for biodegradation. This approach would be of particular interest for antimicrobials, with the potential to ameliorate antimicrobial resistance development. Finally, the concept of resistance to xenobiotics in the context of the gut microbiome may deserve further investigation.

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Section: Incorporating the Gut Microbiome In Food Safety Risk Assessm...mentioning

confidence: 99%

Incorporating the Gut Microbiome in the Risk Assessment of Xenobiotics and Identifying Beneficial Components for One Health

et al. 2022

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“…Hence, three-dimensional models are required to mimic the gut microenvironment dynamically and to investigate its physiology and pathology properly. Gut-on-a-chip (GoC) platforms are favorable to replicate gut dynamics by consistently perfused microchannels and the utilization of several intestinal cell types to mimic the in vivo morphology of the gut [ 375 ]. Maurer et al developed an intestine-on-a-chip platform to understand microbial interactions in the gut microbiota by replicating the immune tolerance of the intestinal lumen with characteristics of mucosal macrophages and dendritic cells [ 376 ].…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Biomedical Applications of Microfluidic Devices: A Review

et al. 2022

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Both passive and active microfluidic chips are used in many biomedical and chemical applications to support fluid mixing, particle manipulations, and signal detection. Passive microfluidic devices are geometry-dependent, and their uses are rather limited. Active microfluidic devices include sensors or detectors that transduce chemical, biological, and physical changes into electrical or optical signals. Also, they are transduction devices that detect biological and chemical changes in biomedical applications, and they are highly versatile microfluidic tools for disease diagnosis and organ modeling. This review provides a comprehensive overview of the significant advances that have been made in the development of microfluidics devices. We will discuss the function of microfluidic devices as micromixers or as sorters of cells and substances (e.g., microfiltration, flow or displacement, and trapping). Microfluidic devices are fabricated using a range of techniques, including molding, etching, three-dimensional printing, and nanofabrication. Their broad utility lies in the detection of diagnostic biomarkers and organ-on-chip approaches that permit disease modeling in cancer, as well as uses in neurological, cardiovascular, hepatic, and pulmonary diseases. Biosensor applications allow for point-of-care testing, using assays based on enzymes, nanozymes, antibodies, or nucleic acids (DNA or RNA). An anticipated development in the field includes the optimization of techniques for the fabrication of microfluidic devices using biocompatible materials. These developments will increase biomedical versatility, reduce diagnostic costs, and accelerate diagnosis time of microfluidics technology.

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“…Another innovative strategy toward more advanced gut-on-chip models is through the integration of organoid technologies [ 186 ] (Figures 4(e) and 4(f) ). As mentioned in the previous section, gut organoids have the advantages of higher cellular complexity and diversity for the study of the gut microbiome.…”

Section: Materials-enabled New Models For Studying Human Gut Microbiomementioning

confidence: 99%

Material Engineering in Gut Microbiome and Human Health

et al. 2022

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Tremendous progress has been made in the past decade regarding our understanding of the gut microbiome’s role in human health. Currently, however, a comprehensive and focused review marrying the two distinct fields of gut microbiome and material research is lacking. To bridge the gap, the current paper discusses critical aspects of the rapidly emerging research topic of “material engineering in the gut microbiome and human health.” By engaging scientists with diverse backgrounds in biomaterials, gut-microbiome axis, neuroscience, synthetic biology, tissue engineering, and biosensing in a dialogue, our goal is to accelerate the development of research tools for gut microbiome research and the development of therapeutics that target the gut microbiome. For this purpose, state-of-the-art knowledge is presented here on biomaterial technologies that facilitate the study, analysis, and manipulation of the gut microbiome, including intestinal organoids, gut-on-chip models, hydrogels for spatial mapping of gut microbiome compositions, microbiome biosensors, and oral bacteria delivery systems. In addition, a discussion is provided regarding the microbiome-gut-brain axis and the critical roles that biomaterials can play to investigate and regulate the axis. Lastly, perspectives are provided regarding future directions on how to develop and use novel biomaterials in gut microbiome research, as well as essential regulatory rules in clinical translation. In this way, we hope to inspire research into future biomaterial technologies to advance gut microbiome research and gut microbiome-based theragnostics.

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Gut-on-Chip microphysiological systems: Latest advances in the integration of sensing strategies and adoption of mature detection mechanisms

Cited by 25 publications

References 168 publications

Incorporating the Gut Microbiome in the Risk Assessment of Xenobiotics and Identifying Beneficial Components for One Health

Incorporating the Gut Microbiome in the Risk Assessment of Xenobiotics and Identifying Beneficial Components for One Health

Biomedical Applications of Microfluidic Devices: A Review

Material Engineering in Gut Microbiome and Human Health

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