Growth suppression of malignant leukemia cell line in vitro by ascorbic acid (vitamin C) and its derivatives

Roomi, M. Waheed; House, Dennis E.; Eckert‐Maksić, Mirjana; Maksić, Zvonimir B.; Tsao, Constance S.

doi:10.1016/s0304-3835(97)00376-5

Cited by 33 publications

(21 citation statements)

References 16 publications

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“…Ascorbic acid has been reported to have cytotoxic and antimetastatic effects on various malignant cell lines [21][22][23][24][25]; in addition, low levels of ascorbic acid have been reported in cancer patients [26]. Green tea extract is a potent anticancer agent that has been reported to have a growth inhibitory effect against certain human cancer cell lines [27][28][29].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of cell invasion and MMP production by a nutrient mixture in malignant liposarcoma cell line SW-872

et al. 2007

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Liposarcoma, a malignancy of fat cells, is the most common soft tissue sarcoma. Though rare, poorly differentiated liposarcomas commonly metastasize to lungs and liver, leading to poor prognosis. Prevention of Extracellular matrix (ECM) degradation by inhibition of matrix metalloproteinases (MMPs) activity has been shown to be a promising therapeutic approach to inhibition of cancer progression. A nutrient mixture (NM) containing lysine, proline, ascorbic acid, and green tea extract has shown significant anticancer activity against a number of cancer cell lines. We investigated the effect of NM on liposarcoma cell line SW-872 proliferation (MTT assay), MMP secretion (gelatinase zymography), invasion through Matrigel, and apoptosis and morphology (live green caspase kit and H&E). Liposarcoma cell growth was inhibited by 36 and 61% at 500 and 1,000 microg/ml NM. Zymography demonstrated both MMP-2 and MMP-9 secretion, with PMA-enhanced MMP-9 activity. NM inhibited both MMPs with virtual total inhibition at 500 microg/ml NM. Invasion through Matrigel was inhibited at 100, 500, and 1,000 microg/ml by 44, 75, and 100%, respectively. Dose-dependent apoptosis of liposarcoma cells was evident with NM challenge, with virtually all cells exposed to 1,000 microg/ml NM in late apoptosis. H&E staining did not demonstrate any changes in morphology at lower concentrations. However, some apoptotic changes were evident at higher concentrations. In conclusion, NM significantly inhibited liposarcoma cell growth, MMP activity, and invasion and induced apoptosis in vitro-important parameters for cancer development, suggesting NM as a potential treatment strategy for liposarcoma.

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Section: Discussionmentioning

confidence: 99%

Inhibition of cell invasion and MMP production by a nutrient mixture in malignant liposarcoma cell line SW-872

et al. 2007

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“…Asc2P administration accomplishes a more marked enrichment of Asc in than administration of Asc itself; this is because Asc2P is considered to scarcely give such deteriorating pro-oxidant eects as those of administered Asc in the extracellular space (Tsao andYoung 1995;Roomi et al 1998) and is considered not able to avoid esterolytic conversion to a free Asc Fig. 6a±d Inhibitory eects of Asc2P or Asc on gelatinase activities in secretes from HT-1080 cells.…”

Section: Discussionmentioning

confidence: 99%

Tumor invasion is inhibited by phosphorylated ascorbate via enrichment of intracellular vitamin C and decreasing of oxidative stress

Nagao¹,

Nakayama²,

Etoh³

et al. 2000

Journal of Cancer Research and Clinical Oncology

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Tumor metastasis and invasion were shown to be inhibited by the 2-O-phosphorylated form (Asc2P) of L-ascorbic acid (Asc); intact Asc did not inhibit tumor invasion when added once, but appreciably inhibited it upon repeated addition. The anti-metastatic effect is attributable to a marked enrichment of intracellular Asc by Asc2P, subsequently dephosphorylated. Asc2P scavenged most of the intracellular reactive oxygen species (ROSin), and notably inhibited production of matrix metalloproteases and cell motility. ROSin was decreased by Asc2P more markedly than by Asc added once. Thus, involvement of ROSin in tumor invasion and a potent anti-metastatic therapy by ROSin-decreasing agents are suggested.

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“…However, the tumour growth-inhibiting effect of ascorbic acid has been demonstrated in several epidemiological and experimental studies [1,3,4]. While many reports have shown the cytotoxic effects of ascorbic acid on a number of malignant and non-malignant cell lines in vitro and in vivo, much less is known about its inhibitory effect on the formation of metastases by cancer cells [4][5][6][7][8]. Metastasis is a complex multistep process, and is a major cause of death amongst cancer patients.…”

Section: Introductionmentioning

confidence: 99%

Ascorbic acid inhibits the migration of walker 256 carcinosarcoma cells

Wybieralska

Koza

Sroka

et al. 2008

Cellular and Molecular Biology Letters

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Abstract:The results of several experimental studies have shown that ascorbic acid inhibits tumor growth and metastasis. Ascorbic acid is an antioxidant that acts as a scavenger for a wide range of reactive oxygen species (ROS). Both tumour metastasis and cell migration have been correlated with the intracellular ROS level, so it was postulated that the inhibitory effect of ascorbic acid derivatives on cell motility may be caused by scavenging of ROS. Time-lapse analyses of Walker 256 carcinosarcoma cell migration showed that both the speed of movement and the cell displacement were inhibited by ascorbic acid applied in concentrations ranging from 10 to 250 μM. This effect correlated with a reduction in the intracellular ROS level in WC 256 cells, suggesting that ROS scavenging may be a mechanism responsible for the inhibition of WC 256 cell migration. However, another potent antioxidant, N-acetyl-L-cysteine, also efficiently decreased the intracellular ROS level in WC 256 cells, but did not affect the migration of the investigated cells. These results demonstrate that intact, unmodified ascorbic acid applied in physiologically relevant and nontoxic concentrations exerts an inhibitory effect on the migration of WC 256 carcinosarcoma cells, and that this may be one of the factors responsible for the anti-metastatic activity of vitamin C. However, our data does not support the hypothesis that the scavenging of intracellular ROS is the main mechanism in the inhibition of cancer cell migration by ascorbic acid.

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Growth suppression of malignant leukemia cell line in vitro by ascorbic acid (vitamin C) and its derivatives

Cited by 33 publications

References 16 publications

Inhibition of cell invasion and MMP production by a nutrient mixture in malignant liposarcoma cell line SW-872

Inhibition of cell invasion and MMP production by a nutrient mixture in malignant liposarcoma cell line SW-872

Tumor invasion is inhibited by phosphorylated ascorbate via enrichment of intracellular vitamin C and decreasing of oxidative stress

Ascorbic acid inhibits the migration of walker 256 carcinosarcoma cells

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