Inhibition of cell invasion and MMP production by a nutrient mixture in malignant liposarcoma cell line SW-872

Roomi, M. Waheed; Ivanov, Vadim; Kalinovsky, Tatiana; Niedzwiecki, Aleksandra; Rath, Matthias

doi:10.1007/s12032-007-0022-z

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…MMP-2 and -9 are soluble MMPs which degrade gelatine and play key roles in tumor angiogenesis, growth and metastasis [11]. Several studies have demonstrated their role in sarcoma progression [7], [39]–[42]. Our results have shown that MMP-2 and -9 were significantly increased in costunolide-treated SW-982 and TE-671 and dehydrocostus-lactone-treated TE-671 cells.…”

Section: Discussionsupporting

confidence: 53%

Sesquiterpene Lactones Downregulate G2/M Cell Cycle Regulator Proteins and Affect the Invasive Potential of Human Soft Tissue Sarcoma Cells

et al. 2013

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Soft tissue sarcomas (STS) represent a rare group of malignant tumors that frequently exhibit chemotherapeutic resistance and increased metastatic potential. Many studies have demonstrated the great potential of plant-derived agents in the treatment of various malignant entities. The present study investigates the effects of the sesquiterpene lactones costunolide and dehydrocostus lactone on cell cycle, MMP expression, and invasive potential of three human STS cell lines of various origins. Both compounds reduced cell proliferation in a time- and dose-dependent manner. Dehydrocostus lactone significantly inhibited cell proliferation, arrested the cells at the G2/M interface and caused a decrease in the expression of the cyclin-dependent kinase CDK2 and the cyclin-dependent kinase inhibitor p27Kip1. In addition, accumulation of cells at the G2/M phase transition interface resulted in a significant decrease in cdc2 (CDK1) together with cyclin B1. Costunolide had no effect on the cell cycle. Based on the fact that STS tend to form daughter cell nests and metastasize, the expression levels of matrix metalloproteinases (MMPs), which play a crucial role in extracellular matrix degradation and metastasis, were investigated by Luminex® technology and real-time RT-PCR. In the presence of costunolide, MMP-2 and -9 levels were significantly increased in SW-982 and TE-671 cells. Dehydrocostus lactone treatment significantly reduced MMP-2 and -9 expression in TE-671 cells, but increased MMP-9 level in SW-982 cells. In addition, the invasion potential was significantly reduced after treatment with both sesquiterpene lactones as investigated by the HTS FluoroBlock™ insert system.

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Section: Discussionsupporting

confidence: 53%

Sesquiterpene Lactones Downregulate G2/M Cell Cycle Regulator Proteins and Affect the Invasive Potential of Human Soft Tissue Sarcoma Cells

et al. 2013

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“…The proteins are known to play a role in the proliferation and migration of normal stem cells and tumor cells (4) and in liposarcoma progression (5). One of the functions of ECM in regulating cell behavior is through their receptors on the cell surface called heterodimer integrins, which consist of a and b subunits (6).…”

Section: Introductionmentioning

confidence: 99%

Integrin α6high Cell Population Functions as an Initiator in Tumorigenesis and Relapse of Human Liposarcoma

Wang

et al. 2011

Molecular Cancer Therapeutics

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The relapse and resistance to chemo-and radiotherapy are main problems in the treatment of human liposarcoma. It is important to find a functional marker existing in the liposarcoma cells for targeting. In this article, we established a new sub-cell line SW872-S cells with high tumorigenicity from human liposarcoma SW872 cells by repeated inoculation approach. The characteristic of the sub-cell line is linked to the high levels of integrin a6 on the surface. The integrin a6 high cells show much higher tumor initiation and self-renewal potential in vivo than integrin a6 low cells do. Targeting integrin a6 with its specific short interfering RNA and antibody significantly inhibits the cell adhesion to laminin and the tumor growth in vitro and in vivo, respectively. Interestingly, integrin a6 marks almost all of the surgical biopsy specimens of patients with liposarcoma relapse. Moreover, integrin a6 is found to coexpress with CD13, which might contribute to the antiapoptosis ability of integrin a6 high cells. Consistently, integrin a6 high cells are more sensitive to the CD13 inhibitor bestatin, and 61% of 23 other human tumor cell lines also contain integrin a6 high CD13 high subgroup.These results provide evidence, for the first time, to our knowledge, that integrin a6 and CD13 can serve as functional markers of the tumor-initiation subcell population in human liposarcoma as well as other cancers for therapeutic targeting. Mol Cancer Ther; 10(12); 2276-86. Ó2011 AACR.

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“…Similar results were obtained with in vitro cancer models in which 1000 μg/ml NM resulted in growth inhibition of various cancer cell lines as seen in Table 2. The breast cancer cell line MDA-MB-231 [35] showed a growth inhibition of 50%, while melanoma A2058 [36] and liposarcoma SW-872 [37] showed inhibition of 64% and 61%, respectively. A relatively lower growth inhibition was seen in the osteosarcoma cell line MNNG which showed an inhibition of only 10% [38].…”

Section: Discussionmentioning

confidence: 98%

Induction of Apoptosis in the Human Prostate Cancer Cell Line DU-145 by a Novel Micronutrient Formulation

Roomi¹,

Shanker²,

Niedzwiecki³

et al. 2015

OJApo

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Prostate cancer, the most frequently diagnosed cancer in men, primarily affects males aged 55 and older and is more common in African Americans than Caucasians. Once the cancer has metastasized, current treatments are generally ineffective. We have identified a novel anti-neoplastic agent, a specifically designed nutrient mixture (NM), containing ascorbic acid, lysine, proline and green tea extract that demonstrates a broad spectrum of anti-tumor activity against a number of human cancer cell lines. In a previous study NM significantly inhibited prostate tumor in nude mice. In this study, we tested whether the formulation exerts its anti-tumor effects through induction of apoptosis on prostate cancer cell line DU-145. The effect of the nutrient mixture (NM) on cell growth inhibition in DU-145 cells was examined by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Morphological changes and caspase activation associated with apoptosis induction was checked by H&E staining and Live Green Caspase assay, respectively. The NM was found to be slightly toxic to DU-145 cells at 100 μg/ml, but significantly toxic at 500 μg/ml and 1000 μg/ml. Percentage of cells undergoing apoptosis also increased from 6% at 100 μg/ml to 49% at 500 μg/ml and 83% at 1000 μg/ml, with greater number of cells showing morphological changes such as condensed nuclei and an acidophilic cytoplasm at higher concentrations. For the purpose of comparison, NM was also tested on a normal human dermal fibroblast (NHDF) cell line which exhibited far less apoptosis induction as compared to DU-145 cells. The percentage of cells undergoing apoptosis in case of NHDF cells was 7% at 100 μg/ml, 25.6% at 500 μg/ml and 76.5% at 1000 μg/ml. Our results demonstrate that the NM is effective in inhibiting cancer cell viability and inducing apoptosis in prostate cancer DU-145 cells and can thus be used as an effective treatment for prostate cancer.

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Inhibition of cell invasion and MMP production by a nutrient mixture in malignant liposarcoma cell line SW-872

Cited by 9 publications

References 21 publications

Sesquiterpene Lactones Downregulate G2/M Cell Cycle Regulator Proteins and Affect the Invasive Potential of Human Soft Tissue Sarcoma Cells

Sesquiterpene Lactones Downregulate G2/M Cell Cycle Regulator Proteins and Affect the Invasive Potential of Human Soft Tissue Sarcoma Cells

Integrin α6high Cell Population Functions as an Initiator in Tumorigenesis and Relapse of Human Liposarcoma

Induction of Apoptosis in the Human Prostate Cancer Cell Line DU-145 by a Novel Micronutrient Formulation

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