Growth potential of cottonseed culture media for various clinically significant aerobic bacteria

Slifkin, Malcolm; Pouchet, Gail R.

doi:10.1128/jcm.1.6.495-499.1975

Cited by 6 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For cultivation of edible and/or medicinal mushrooms, the substrate rich in essential bio-available nutrients with optimum pH, moisture, and air content is preferred ( Chang & Wasser, 2018 ). Cottonseed hulls provide fine water holding capacity and nitrogen to ensure the water and nutrient supply for the cultivation of edible mushrooms such as Pleurotus ostreatus ( Lu & Qu, 1984 ) and aerobic bacteria ( Slifkin & Pouchet, 1975 ). The mixture of cottonseed hulls with other materials such as wheat bran, corn flour and gypsum was used for industrial production of G. lucidum in China.…”

Section: Discussionmentioning

confidence: 99%

Dynamic succession of substrate-associated bacterial composition and function duringGanoderma lucidumgrowth

Zhang

Yan

et al. 2018

PeerJ

View full text Add to dashboard Cite

BackgroundGanoderma lucidum, a valuable medicinal fungus, is widely distributed in China. It grows alongside with a complex microbial ecosystem in the substrate. As sequencing technology advances, it is possible to reveal the composition and functions of substrate-associated bacterial communities.MethodsWe analyzed the bacterial community dynamics in the substrate during the four typical growth stages of G. lucidum using next-generation sequencing.ResultsThe physicochemical properties of the substrate (e.g. acidity, moisture, total nitrogen, total phosphorus and total potassium) changed between different growth stages. A total of 598,771 sequences from 12 samples were obtained and assigned to 22 bacterial phyla. Proteobacteria and Firmicutes were the dominant phyla. Bacterial community composition and diversity significantly differed between the elongation stage and the other three growth stages. LEfSe analysis revealed a large number of bacterial taxa (e.g. Bacteroidetes, Acidobacteria and Nitrospirae) with significantly higher abundance at the elongation stage. Functional pathway prediction uncovered significant abundance changes of a number of bacterial functional pathways between the elongation stage and other growth stages. At the elongation stage, the abundance of the environmental information processing pathway (mainly membrane transport) decreased, whereas that of the metabolism-related pathways increased.DiscussionThe changes in bacterial community composition, diversity and predicted functions were most likely related to the changes in the moisture and nutrient conditions in the substrate with the growth of G. lucidum, particularly at the elongation stage. Our findings shed light on the G. lucidum-bacteria-substrate relationships, which should facilitate the industrial cultivation of G. lucidum.

show abstract

Section: Discussionmentioning

confidence: 99%

Dynamic succession of substrate-associated bacterial composition and function duringGanoderma lucidumgrowth

Zhang

Yan

et al. 2018

PeerJ

View full text Add to dashboard Cite

show abstract

“…For cultivation of edible and/or medicinal mushrooms, the substrate rich in essential bio-available nutrients with optimum pH, moisture, and air content is preferred (Chang & Wasser 2017). Cottonseed hulls provide fine water holding capacity and nitrogen to ensure the water and nutrient supply for the cultivation of edible mushrooms such as Pleurotus ostreatus (Lu & Qu, 1984) and aerobic bacteria (Slifkin & Pouchet G, 1975). The mixture of cottonseed hulls with other materials such as wheat bran, corn flour and gypsum was used for industrial production of G. lucidum in China.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #2 of "Dynamic succession of substrate-associated bacterial composition and function during Ganoderma lucidum growth (v0.2)"

Ayayee¹

2018

View full text Add to dashboard Cite

Background Ganoderma lucidum, a valuable medicinal fungus, is widely distributed in China. It grows alongside with a complex microbial ecosystem in the substrate. As sequencing technology advances, it is possible to reveal the composition and functions of substrate-associated bacterial communities. Methods We analyzed the bacterial community dynamics in the substrate during the four typical growth stages of G. lucidum using next-generation sequencing. Results The physicochemical properties of the substrate (e.g. acidity, moisture, total nitrogen, total phosphorus and total potassium) changed between different growth stages. A total of 598,771 sequences from 12 samples were obtained and assigned to 22 bacterial phyla. Proteobacteria and Firmicutes were the dominant phyla. Bacterial community composition and diversity significantly differed between the elongation stage and the other three growth stages. LEfSe analysis revealed a large number of bacterial taxa (e.g. Bacteroidetes, Acidobacteria and Nitrospirae) with significantly higher abundance at the elongation stage. Functional pathway prediction uncovered significant abundance changes of a number of bacterial functional pathways between the elongation stage and other growth stages. At the elongation stage, the abundance of the environmental information processing pathway (mainly membrane transport) decreased, whereas that of the metabolism-related pathways increased. Discussion The changes in bacterial community composition, diversity and predicted functions were most likely related to the changes in the moisture and nutrient conditions in the substrate with the growth of G. lucidum, particularly at the elongation stage. Our findings shed light on the G. lucidum-bacteria-substrate relationships, which should facilitate the industrial cultivation of G. lucidum.

show abstract

Feasibility of incorporating cotton seed extract in Clostridium strain P11 fermentation medium during synthesis gas fermentation

Kundiyana

Huhnke

Maddipati

et al. 2010

Bioresource Technology

View full text Add to dashboard Cite

Growth potential of cottonseed culture media for various clinically significant aerobic bacteria

Cited by 6 publications

References 27 publications

Dynamic succession of substrate-associated bacterial composition and function duringGanoderma lucidumgrowth

Dynamic succession of substrate-associated bacterial composition and function duringGanoderma lucidumgrowth

Peer Review #2 of "Dynamic succession of substrate-associated bacterial composition and function during Ganoderma lucidum growth (v0.2)"

Feasibility of incorporating cotton seed extract in Clostridium strain P11 fermentation medium during synthesis gas fermentation

Contact Info

Product

Resources

About