Growth of Rift Valley Fever Virus in Human Diploid (Wi-38) Cells

Evaluation of Factors Related to Growth of Rift Valley Fever Virus in Suspended Cell Cultures

“…Orlando et al (12), of these laboratories, reported that the optimal virus input multiplicity was approximately 2.5. More recently, Johnson et al (9), using a monolayer tissue culture system of mouse fibroblast-like cells, described the growth of RVFV and reported on optimal virus input multiplicities and medium volume for this system. They also developed and described growth curves for both monolayer and suspension systems.…”

Growth of Venezuelan Equine Encephalomyelitis Virus in Human Diploid Cell Strain WI-38

“…Diploid cell strains have supported the replication of human and animal viruses (5,6,14). It was of interest to us to investigate the ability of WI-38 cells to support the growth of VEE virus as a possible method for the production of virus for the preparation of inactivated vac-…”