Evaluation of Factors Related to Growth of Rift Valley Fever Virus in Suspended Cell Cultures

Walker, Jerry S.; Carter, Richard; Klein, Frederick; Snowden, Shirley E.; Lincoln, Ralph E.

doi:10.1128/aem.17.5.658-664.1969

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…When tested with the appropriate controls, it was found that calf serum as the sole supplement at a concentration of 5% did not enhance JEV production. We have, therefore, substantiated the work of Tribble et al (28) and Walker et al (29) who concluded that serum is not a prerequisite for the replication of several other arboviruses in other host cell systems. The second supplement tested, a mixture of nonessential amino acids, consisting of 0.5 mM concentrations of L-proline, L-serine, L-aspartic acid, L-asparagine, Lalanine, L-glutamic acid, and glycine, neither enhanced nor inhibited JEV production in BHK-21 cells suspended in MEM with 20 mM HEPES buffer.…”

Section: Discussionsupporting

confidence: 83%

Adaptation of BHK-21 Cells to Growth in Shaker Culture and Subsequent Challenge by Japanese Encephalitis Virus

Guskey¹,

Jenkin²

1975

Applied Microbiology

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Section: Discussionsupporting

confidence: 83%

Adaptation of BHK-21 Cells to Growth in Shaker Culture and Subsequent Challenge by Japanese Encephalitis Virus

Guskey¹,

Jenkin²

1975

Applied Microbiology

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“…Therefore inactivated RVF vaccine was prepared locally using RVFV with MOI 0.1 to 0.01 according to El-Nimr (1980). The time of peak titer was dependent on MOI and initial cell concentration (Walker et al, 1969).…”

Section: Resultsmentioning

confidence: 99%

Modified Method for Propagation of Rift Valley Fever Virus

2015

NIDOC-ASRT

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rials for propagation of RVFV on Vero cells using different MOI .. ..0.1, 0.01 & 0.001 and different concentrations of trypsin (25 & 50 µg/ml). The result indicated that high titer of RVFV (8.0 log 10 TCID 50 /ml in Vero cells, 7.5 log 10 MIPLD 50 /ml in adult mice and 7.7 log 10 MICLD 50 /ml in baby mice) was obtained with MOI 0.001 using 50 µg / ml of trypsin after 24 hrs post inoculation. Inoculation of freshly subcultured Vero cells as other method lead to obtaining high virus titer (8.5 log 10 TCID 50 /ml in Vero cells, 7.6 log 10 MIPLD 50 /ml in adult mice and 8.2 log 10 MICLD 50 /ml in baby mice) after 48 hours with MOI 0.0001. While using subculture of monolayer Vero cells 24 hours previously infected with RVFV, it was obtained high titer (8.0 log 10 TCID 50 /ml in Vero cells, 7.4 log 10 MIPLD 50 / ml in adult mice and 7.9 log 10 MICLD 50 /ml in baby mice) with MOI 0.001 as well as a triple amount of virus yielded than the traditional method. Different batches of inactivated RVFV vaccine were prepared from the harvested virus which was identified as RVFV using AGPT. Quality control tests were applied proved that all vaccine batches were sterile, safe and potent. It is concluded that modified methods could increase RVFV production with high titer as well as saving time, saving materials used in virus production include quantity of inoculums.

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“…of Pretoria, Pretoria, South Africa) of RVF virus was used. Origin and maintenance of this strain were described earlier (20). The African Chik-2 strain of CHIK virus, harvested 42 hr postinfection, was prepared as a 10% mouse brain suspension in medium 199 containing 400 units of penicillin and 400 ,ug of streptomycin per ml.…”

mentioning

confidence: 99%

Concentration of Rift Valley Fever and Chikungunya Viruses by Precipitation

Klein¹,

Mahlandt²,

Cockey³

et al. 1970

Applied Microbiology

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Simple and efficient methods for concentrating Rift Valley fever (RVF) virus and chikungunya (CHIK) virus are described. Ammonium sulfate, potassium sulfate, or alcohol was used as a precipitating agent and the precipitate was resuspended to volumes suitable for further processing and purification. The methods permitted concentration of live RVF virus and CHIK virus about 100-fold with negligible losses of virus. RVF virus retained a high level of infectivity with potassium aluminum sulfate and alcohol, but CHIK virus retained a higher infectivity level with ammonium sulfate than with potassium aluminum sulfate. The data indicate that serum plays an important role in the concentration of both viruses, at least when the sulfate methods are used. Certain viruses have been concentrated by adsorption to salts and molecular gels, e.g., polioviruses on aluminum hydroxide gels (14, 16) and on calcium and aluminum phosphate gel (19), fowl plaque influenza, Newcastle, and mumps viruses on calcium phosphate (3, 15) or aluminum

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Evaluation of Factors Related to Growth of Rift Valley Fever Virus in Suspended Cell Cultures

Cited by 6 publications

References 8 publications

Adaptation of BHK-21 Cells to Growth in Shaker Culture and Subsequent Challenge by Japanese Encephalitis Virus

Adaptation of BHK-21 Cells to Growth in Shaker Culture and Subsequent Challenge by Japanese Encephalitis Virus

Modified Method for Propagation of Rift Valley Fever Virus

Concentration of Rift Valley Fever and Chikungunya Viruses by Precipitation

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