Transmission of infection with adenovirus type 37 was successfully interrupted following strict infection control, suspension of new admissions, cohorting of residents by unit, and change to a disinfectant that inactivates adenovirus. Recognition of conjunctivitis as an appropriate reason for restricting movement of an infected resident may have prevented extensive viral transmission in this outbreak.

SUMMARYThe viral inhibitors guanidine hydrochloride and 2-(~-hydroxybenzyl)-benzimidazole (HBB) were used to study the relationship between lysosomal enzyme release and the development of poliovirus-induced cytopathic effect (CPE) in HEp-2 cells. Lysosomal enzyme release, the development of CPE and virus replication were inhibited or delayed if these antiviral agents were added up to 2 to 3 hr after infection. When added later the agents were no longer effective. It is suggested that a virus-induced protein produced during 3 hr after infection is responsible for lysosomal enzyme release.

SUMMARYStructural proteins from a large-plaque variant (LPV) of human parainfluenza type 3 virus were analysed by electrophoresis on Laemmli-type polyacrylamide gels. High virus concentrations were obtained by growth in BS-C-1 cells cultivated on microcarrier beads. Purification of the virus in composite equilibrium gradients of potassium tartrate:glycerol resulted in 25% recovery of input infectivity and a preparation containing <0.08 % of input host cell protein and RNA. Parainfiuenza type 3 virus equilibrated at a density of 1.20 g/ml in these gradients. Analysis by polyacrylamide gel electrophoresis of 3H-glucosamine-labelled virus taken from peak gradient fractions revealed 8 or 9 major virion peptides, ranging in mol. wt. from 17 x 103 to 125 x 103 (17K to 125K), two of which were glycoproteins. The sum of the estimated mol. wt. of these peptides, 501.5K to 570.5K, does not exceed the estimated genomic potential of other paramyxoviruses.

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