Growth of Venezuelan Equine Encephalomyelitis Virus in Human Diploid Cell Strain WI-38

Reitman, Morton; Green, Leonard

doi:10.1128/aem.19.1.196-198.1970

Cited by 2 publications

(2 citation statements)

References 7 publications

(8 reference statements)

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“…Preparation of vaccines. Tissue culture virus suspensions were prepared in chick fibroblast monolayers, in Maitland-type chick embryo suspensions (MTCE) as described previously (15), and in monolayers of WI-38 cells (16). Virus suspensions were clarified by centrifuging at 700 X g in a PR-2 (I.E.C.)…”

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Gamma-Irradiated Venezuelan Equine Encephalitis Vaccines

Reitman¹,

Tribble²,

Jr.³

et al. 1970

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The efficacy of Formalin-inactivated Venezuelan equine encephalitis (VEE) vaccine has been reported to be low for man. Although a live VEE vaccine has been shown to be highly effective for the protection of laboratory workers, local and systemic reactions have occurred in approximately 20% of inoculated individuals. Therefore, studies were initiated in an attempt to produce an inactivated vaccine of high potency with low toxicity. Inactivated VEE vaccines were prepared by exposing virus suspensions to 8 x 106 or 10 X 106 r of gamma radiation. Irradiated VEE vaccines prepared from virus suspensions produced in Maitland-type chick embryo (MTCE) cell cultures and in monolayer cultures of human diploid strain WI-38 cells were highly immunogenic for mice and guinea pigs. Guinea pigs vaccinated with a series of three inoculations of vaccine (MTCE) survived challenge with at least 108.4 mouse intracerebral 50% lethal doses of VEE virus. Irradiated vaccines induced high levels of serum-neutralizing and hemagglutinin-inhibiting antibodies in guinea pigs and rabbits. These findings suggest that ionizing radiation may be effective in the preparation of an inactivated VEE vaccine.

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confidence: 99%

Gamma-Irradiated Venezuelan Equine Encephalitis Vaccines

Reitman¹,

Tribble²,

Jr.³

et al. 1970

Self Cite

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“…Ten per cent or more of the animals were bled intracardially before vaccination, 21 days postvaccination, and 14 days postchallenge. Serum samples were assayed for hemagglutinatinginhibiting (HI; reference 14) and serum-neutralizing (SN) antibodies as previously described (15). SN indexes are expressed as log10 ; HI titers are expressed as the reciprocal.…”

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confidence: 99%