Growth of Helicobacter pylori in various liquid and plating media

Abstract

The objectives of this research were to compare commonly used liquid and plating media to elucidate whether one medium provided superior growth of Helicobacter pylori in vitro. The liquid media compared were Mueller‐Hinton broth, brain heart infusion broth and H. pylori special peptone broth, formulated in this laboratory. No significant differences in growth rates were noted and shaking during the incubation of broths was not essential for good growth. The plating media compared included Columbia agar, Mueller‐Hinton agar, modified Glupczynski's Brussels campylobacter charcoal agar, Johnson‐Murano agar and H. pylori special peptone agar (HPSPA). None of the non‐specific plating media that have been used historically to culture H. pylori exhibited any particular advantage. However, HPSPA provided an obvious advantage in colony size. Helicobacter pylori special peptone agar enhances the cultivation of H. pylori and could improve the recovery of the bacterium from clinical samples in vitro.

Optimization and Culture Conditions to Improve Helicobacter Pylori Growth in HAM's F-12 Medium by Response Surface Methodology

“…In fact, in our model, shaking significantly influenced growth and the value of 130 rpm was found to be optimal. This result did not support the observations of Stevenson and collaborators (32) who mentioned that shaking was not essential for good growth. They explained their results by the small volume used, the method of establishing a microaerophilic atmosphere and the area of gas interface with the medium.…”

Evaluation of a Nested-PCR assay based on the phosphoglucosamine mutase gene (glmM) for the detection of Helicobacter pylori from raw milk

“…However, since isolation of H. pylori from foods is extremely difficult due to the presence of accompanying microflora and to the presumably very low H. pylori load contaminating foodstuff (Cellini, Del Vecchio et al, 2004;Dunn et al, 1997;Stevenson et al, 2000), it is important to evaluate specific, sensitive and rapid methods for the detection of this pathogen from food products, and in particular from milk.…”

“…This lack of data could be accounted for the difficulty in H. pylori isolation from foods, particularly in presence of a high load of accompanying microflora. In fact it is exacting and time-consuming since it requires the employment of selective media supplemented with numerous antibiotics, microaerophilic conditions and a long incubation periods (seven days) (Stevenson, Castillo, Lucia, & Acuff, 2000). Furthermore, H. pylori may produce viable nonculturable forms (VNC) (Cellini, Del Vecchio et al, 2004;Dunn, Cohen, & Blaser, 1997) not detectable by means of conventional microbiological techniques; however, it has been hypothesized that VNC forms are still infective (Bode, Mauch, & Malfertheiner, 1993;Cao, Li, Borch, Petersson, & Mardh, 1997) thus representing a potential microbiological risk for consumers.…”

A Comparative Study ofHelicobacter pyloriGrowth on Different Agar-based Media

“…3,4 However, there are no studies that compare the effectiveness of these media by repeated experiments. 3,7,10,11 Therefore, we aimed to compare and contrast four agar-based media (chocolate media, brucella media, BHI media and Thayer-Martin [TM] agar) for allowing efficient growth of H. pylori and to find the most suitable one for laboratory use.…”