Evaluation of a Nested-PCR assay based on the phosphoglucosamine mutase gene (glmM) for the detection of Helicobacter pylori from raw milk

Quaglia, Nicoletta Cristiana; Dambrosio, Angela; Normanno, G.; Celano, Gaetano Vitale

doi:10.1016/j.foodcont.2008.02.010

Cited by 11 publications

(17 citation statements)

References 43 publications

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“…It has been previously reported that detecting this gene improves sensitivity and specificity of recognition of H. pylori in samples containing prokaryotic cells as well as many organic impurities [9, 17, 37]. However, because the PCR assay detects ureC ( glmM ) gene of H. pylori , we are unable to speculate on the viability of organisms in water samples.…”

Section: Resultsmentioning

confidence: 81%

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Detection of Helicobacter pylori in City Water, Dental Units′ Water, and Bottled Mineral Water in Isfahan, Iran

Bahrami

Rahimi

Safaei

2013

The Scientific World Journal

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Helicobacter pylori infection in human is one of the most common infections worldwide. However, the origin and transmission of this bacterium has not been clearly explained. One of the suggested theories is transmission via water. This study was conducted to determine the prevalence rate of H. pylori in tap water, dental units' water, and bottled mineral water in Iran. In the present study, totally 200 water samples were collected in Isfahan province and tested for H. pylori by cultural method and polymerase chain reaction (PCR) by the detection of the ureC (glmM) gene. Using cultural method totally 5 cultures were positive. Two out of 50 tap water samples (4%), 2 out of 35 dental units' water (5.8%) samples, and 1 out of 40 (2.5% ) from water cooler in public places were found to be contaminated with H. pylori. H. pylori ureC gene was detected in 14 (7%) of water samples including 5 tap water (10%), 4 dental units' water (11.4%), 1 refrigerated water with filtration, and 4 (10%) water cooler in public places samples. This may be due to the coccoid form of bacteria which is detected by PCR method.

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Section: Resultsmentioning

confidence: 81%

“…H. pylori DNA has been identified in several water sources using diverse gene targets. Drinking, river, sea, ground, and wastewater have provided positive results by PCR analysis [9, 14, 23, 24, 35–37]. The H. pylori DNA present in water samples could be from dead H. pylori cells or from VBNC forms, since culture is usually not possible.…”

Section: Resultsmentioning

confidence: 99%

Detection of Helicobacter pylori in City Water, Dental Units′ Water, and Bottled Mineral Water in Isfahan, Iran

Bahrami

Rahimi

Safaei

2013

The Scientific World Journal

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“…There is indirect hypothesis of H. pylori transmission through milk, similar to that obtained for water, but less extensive [ 4 , 6 , 22 – 24 ]. These studies led to the hypothesis that H. pylori infection can be considered a zoonosis, which is further reinforced by the occurrence of this bacterium in the gastric mucosa of calves, pigs, and horses and its isolation from gastric tissue and milk of sheep [ 4 ].…”

Section: Resultsmentioning

confidence: 98%

Virulence factors and antibiotic resistance of Helicobacter pylori isolated from raw milk and unpasteurized dairy products in Iran

Mousavi

Dehkordi

Rahimi

2014

J Venom Anim Toxins incl Trop Dis

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BackgroundDespite the high importance of Helicobacter pylori, the origin and transmission of this bacterium has not been clearly determined. According to controversial theories and results of previous studies, animal source foods – especially milk – play an important role in the transmission of H. pylori to humans. The aim of the present study was to determine the distribution of vacA, cagA, iceA and oipA virulence factors in H. pylori strains isolated from milk and dairy products and study their antimicrobial resistance properties.MethodsA total of 520 raw milk and 400 traditional dairy product samples were cultured and tested. Those that were H. pylori-positive were analyzed for the presence of vacA, cagA, iceA and oipA virulence factors. Antimicrobial susceptibility testing was performed by the disk diffusion method.ResultsOne hundred and three out of 520 milk samples (19.8%) and 77 out of 400 dairy products samples (19.2%) were contaminated with H. pylori. The most frequently contaminated samples were ovine milk (35%) and traditional cheese (30%). Total prevalence of vacA, cagA, iceA and oipA factors were 75%, 76.6%, 41.6% and 25%, respectively. H. pylori strains of milk and dairy products harbored high levels of resistance to ampicillin (84.4%), tetracycline (76.6%), erythromycin (70.5%) and metronidazole (70%).ConclusionsHigh presence of antibiotic-resistant strains of H. pylori suggest that milk and dairy samples may be the sources of bacteria that can cause severe infection. Our findings should raise awareness about antibiotic resistance in H. pylori strains in Iran.

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“…51104, QIAGEN), with slightly modifying the supplier’s instructions according to Quaglia et al . [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

“…Nested PCR was already employed for the detection of H. pylori glmM gene from water and was reported to be as a highly sensitive and specific method for the rapid detection and screening of sheep, goat and cow milk [ 11 - 15 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of Helicobacter pylori glmM gene in bovine milk using Nested polymerase chain reaction

Osman

Eleragi

Musa³

et al. 2015

Vet World

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Aim:The aim was to detect the glmM gene of Helicobacter pylori (H. pylori) in cow’s milk from different dairy farms in Khartoum State using Nested polymerase chain reaction (PCR).Materials and Methods:A total of 50 milk samples were collected from different dairy farms in Khartoum State (13 from Khartoum, 24 Khartoum North, and 13 from Omdurman Provinces).Results:The generated results showed that 11/50 (22%) were harboring the investigated H. pylori glmM gene in Khartoum State (1/13 [7.7%] Khartoum, 9/24 [37.5%] Khartoum North, and 1/13 [7.7%] Omdurman provinces, respectively).Conclusion:To the best of our knowledge, this was the first report on the detection of H. pylori glmM gene in cattle milk in Khartoum State. Nonetheless, the high percentages of H. pylori DNA detection in milk opened new avenues toward exploring the risk of human infection with H. pylori through the consumption of raw milk.

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Evaluation of a Nested-PCR assay based on the phosphoglucosamine mutase gene (glmM) for the detection of Helicobacter pylori from raw milk

Cited by 11 publications

References 43 publications

Detection of Helicobacter pylori in City Water, Dental Units′ Water, and Bottled Mineral Water in Isfahan, Iran

Detection of Helicobacter pylori in City Water, Dental Units′ Water, and Bottled Mineral Water in Isfahan, Iran

Virulence factors and antibiotic resistance of Helicobacter pylori isolated from raw milk and unpasteurized dairy products in Iran

Detection of Helicobacter pylori glmM gene in bovine milk using Nested polymerase chain reaction

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