A Comparative Study of<i>Helicobacter pylori</i>Growth on Different Agar-based Media

Lee, Jung Hwan; Park, Ji Wan; Park, Mi Ri; Na, Yoon Hee; Cho, Soo‐Jeong

doi:10.7704/kjhugr.2017.17.4.208

Cited by 5 publications

(5 citation statements)

References 21 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is assumed that AMPKs evolved in the early eukaryote to control the output of carbohydrates produced by acquired bacterial endosymbionts that developed into mitochondria but were thought not to play a role in bacteria [106][107][108]. However, AMPKs were isolated from L. pneumophila [21], H. pylori [22][23][24], Mycobacterium spp., B. subtilis, L. monocytogenes, and rhizobial bacteria [109][110][111][112] and were involved in sensitization of resistant bacteria to antibiotics, too [10,70,[83][84][85]. Bacterial AMPKs may be inhibited by metformin.…”

Section: Inhibition Of Bacterial Serine/threonine/tyrosine Protein Kimentioning

confidence: 99%

“…Copper complexation may also contribute to the antihyperglycaemic action of metformin [19,20]. Metformin exerted effects not only on the gut microbiome but also against Legionella pneumophila [21] and H. pylori [22][23][24] and several other bacterialand viral species [25] in vitro, in vivo and in the clinical setting. Proposed mechanisms of antibacterial action encompass those effects attributed to antihyperglycaemic effects of metformin, inhibition of electron transport, and also AMPK independent effects such as immunomodulation, or production of mitochondrial reactive oxygen species thus enhancing bactericidal activities of macrophages [25,26].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Are antibacterial effects of non-antibiotic drugs random or purposeful because of a common evolutionary origin of bacterial and mammalian targets?

Dalhoff

2020

Infection

View full text Add to dashboard Cite

Purpose Advances in structural biology, genetics, bioinformatics, etc. resulted in the availability of an enormous pool of information enabling the analysis of the ancestry of pro- and eukaryotic genes and proteins. Methods This review summarizes findings of structural and/or functional homologies of pro- and eukaryotic enzymes catalysing analogous biological reactions because of their highly conserved active centres so that non-antibiotics interacted with bacterial targets. Results Protease inhibitors such as staurosporine or camostat inhibited bacterial serine/threonine or serine/tyrosine protein kinases, serine/threonine phosphatases, and serine/threonine kinases, to which penicillin-binding-proteins are linked, so that these drugs synergized with β-lactams, reverted aminoglycoside-resistance and attenuated bacterial virulence. Calcium antagonists such as nitrendipine or verapamil blocked not only prokaryotic ion channels but interacted with negatively charged bacterial cell membranes thus disrupting membrane energetics and inducing membrane stress response resulting in inhibition of P-glycoprotein such as bacterial pumps thus improving anti-mycobacterial activities of rifampicin, tetracycline, fluoroquinolones, bedaquilin and imipenem-activity against Acinetobacter spp. Ciclosporine and tacrolimus attenuated bacterial virulence. ACE-inhibitors like captopril interacted with metallo-β-lactamases thus reverting carbapenem-resistance; prokaryotic carbonic anhydrases were inhibited as well resulting in growth impairment. In general, non-antibiotics exerted weak antibacterial activities on their own but synergized with antibiotics, and/or reverted resistance and/or attenuated virulence. Conclusions Data summarized in this review support the theory that prokaryotic proteins represent targets for non-antibiotics because of a common evolutionary origin of bacterial- and mammalian targets resulting in highly conserved active centres of both, pro- and eukaryotic proteins with which the non-antibiotics interact and exert antibacterial actions.

show abstract

Section: Inhibition Of Bacterial Serine/threonine/tyrosine Protein Kimentioning

confidence: 99%

mentioning

confidence: 99%

Are antibacterial effects of non-antibiotic drugs random or purposeful because of a common evolutionary origin of bacterial and mammalian targets?

Dalhoff

2020

Infection

View full text Add to dashboard Cite

show abstract

“…When comparing with the number of positives obtained by antigenic screening, it can be suggested that the culture method allows determining if the microorganism detected by screening test is viable in the plate, in such a way that the number of viable samples is lower than antigenic screening. In a study conducted by Lee et al (29) after comparing some culture methods from biopsy samples, they determined that the culture media, Thayer agar, Brucella agar, Chocolate agar, and Brain heart infusion agar acted in the same way, obtaining similar results under conditions of 10% CO 2 and 96% humidity at 37°C. Similarly, in a work carried out by Xu et al (30) it was determined that in Brucella agar, chocolate agar and brain heart infusion, H. pylori agar has the ability to live up to 56 days.…”

Section: Discussionmentioning

confidence: 99%

Detection of Helicobacter pylori from Human Biological Samples (Feces) by Antigenic Screening and Culture

Guaman

Bayas-Morejón

Arcos

et al. 2018

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: This research addressed the topic of timely diagnosis of Helicobacter pylori that is currently a problem for public health in Ecuador and worldwide. The prevalence of the pathogen has increased in the last decade, especially due to its direct participation in gastric cancer generation. Objectives: The present investigation was carried out to detect H. pylori in human stool samples by antigenic screening and culture. Methods: The methodology used in this study was direct and indirect analysis of patients with H. pylori infection symptoms. Overall, 50 stool samples were collected (16 from males and 34 from females) and transferred immediately to the laboratory; patients were excluded due to causes such as contamination or insufficient amounts of sample for the analysis. From these samples, antigenic screening and culture in plates were carried out using the Brucella blood agar selective medium under controlled conditions. The two methods were analysed using the Kappa coefficient. Results: After antigenic screening analysis, 25 samples (50%) were positive for H. pylori with four false positives for both genders. The culture results showed that 19 samples (38%) were positive with 32 isolates characteristic for H. pylori. After agreement analysis, the kappa index was 0.28 (discrete). Conclusions:The detection rate by antigenic screening was higher than that obtained by culture, this is mainly due to the lack of viable cells and the difficulty of adapting adequate isolation techniques.

show abstract

“…Cells were washed once with sterile PBS, and then two H. pylori strains (60190 from ATCC, and G27, kindly provided by Prof. Nayoung Kim, Seoul National University, Korea) were added at a multiplicity of infection (MOI) of 100:1 for different time points, followed by washing with PBS six times to remove nonadherent bacteria. Isogenic mutants lacking CagA (60190DA) or CagE (G27DE) were used as described previously (19,20).…”

Section: H Pylori Infection Of Hfe 145 Cellsmentioning

confidence: 99%

CDX1 Expression Induced by CagA-ExpressingHelicobacter pyloriPromotes Gastric Tumorigenesis

Choi

Yoon

Park

et al. 2019

Molecular Cancer Research

Self Cite

View full text Add to dashboard Cite

Intestinal-type gastric cancer often results from Helicobacter pylori infection through intestinal metaplasia, a transdifferentiated premalignant phenotype. Because H. pylori virulence factor CagA has been associated with aberrant expression of the transcription factor CDX1, which regulates intestinal differentiation, we explored its relationship with H. pylori infection and function during gastric carcinogenesis in normal gastric epithelial cells and gastric cancer cell lines. Infection of HFE 145 cells with CagA þ H. pylori increased expression of CDX1, as well as the epithelial-to-mesenchymal transition (EMT) markers Snail and Slug, increased invasion and migration, but those effects were not found in HFE 145 cells infected with CagA-deficient H. pylori. CDX1 overexpression increased expression of the intestinal markers Villin, sucrose isomaltase (SI), and MUC2, induced spheroid formation, and enhanced expression of the stem cell markers CD44, SOX2, Oct4, and Nanog, while CDX1 knockdown inhibited proliferation and intestinal stemness. Treatment of CDX1-expressing cells with metformin, an antidiabetic drug known to decrease the risk of gastric cancer, decreased expression of EMT and stemness markers, and reduced spheroid formation. In a murine xenograft model, combining metformin or shCDX1 with cisplatin reduced tumor growth, increased caspase-3 cleavage, and reduced expression of CD44 and MMP-9 to a greater degree than cisplatin alone. Patients with more advanced intestinal metaplasia staging exhibited higher CDX1 expression than those with earlier intestinal metaplasia staging (P ¼ 0.039), and those with H. pylori tended to have more CDX1 expression than noninfected patients (P ¼ 0.061). Finally, human tissue samples with higher CDX1 levels showed prominent CD44/ SOX2 expression. Our findings indicate CagA þ H. pyloriinduced CDX1 expression may enhance gastric cancer tumorigenesis and progression, and support therapeutic targeting of CDX1 in gastric cancer.Implications: This study shows that CDX1 contributes to the tumorigenesis and progression of gastric cancer and suggests the potential of targeting CDX1 to treat this malignancy.

show abstract

A Comparative Study ofHelicobacter pyloriGrowth on Different Agar-based Media

Cited by 5 publications

References 21 publications

Are antibacterial effects of non-antibiotic drugs random or purposeful because of a common evolutionary origin of bacterial and mammalian targets?

Are antibacterial effects of non-antibiotic drugs random or purposeful because of a common evolutionary origin of bacterial and mammalian targets?

Detection of Helicobacter pylori from Human Biological Samples (Feces) by Antigenic Screening and Culture

CDX1 Expression Induced by CagA-ExpressingHelicobacter pyloriPromotes Gastric Tumorigenesis

Contact Info

Product

Resources

About