Growth Inhibition and Apoptosis Inducing Effects of <i>Artemisia absinthium</i> L. Fractions on Chronic Myeloid Leukemia (K562) Cells

Bhat, Mohd Yasin; Gul, Mir Zahoor; Maurya, Radheshyam; Khan, Nooruddin; Qureshi, Insaf Ahmed; Ghazi, Irfan Ahmad

doi:10.1080/22311866.2018.1461577

Cited by 3 publications

(3 citation statements)

References 46 publications

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“…Apart from this several other unknown peaks were also recognized in both extracts by comparing retention time in already reported literature. This includes Quercitrin, Isolongefinone, β-Myrcene, Rutoside, Allo-Ocimene as reported by [ 12 , 20 , 21 ]. Invanescu et al 2010 reported that compound with retention time 22.54 is Quercetin, and same was evaluated by present results of HPLC.…”

Section: Resultsmentioning

confidence: 90%

Pharmacological activities of Artemisia absinthium and control of hepatic cancer by expression regulation of TGFβ1 and MYC genes

Sohail

Zubaır²,

Hussain³

et al. 2023

PLoS ONE

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Plant derived compounds have always been an important source of medicines and have received significant attention in recent years due to their diverse pharmacological properties. Millions of plant-based herbal or traditional medicines are used to cure various types of cancers especially due to activation of proliferative genes. The aim of the present study was to characterize the altered and attenuated gene expression of the selected growth factor namely Transforming growth factor Beta -1 (TGFβ1) and MYC in human hepatoma-derived (Huh7) liver cancer cell lines in response to extracts of Artemisia absinthium dissolved in selected organic solvents. Ethanolic, methanolic and acetone extract of different plant parts (leaf, stem and flowers) was used to access the antiproliferative activity by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay. Quantitative Real-Time RT-PCR revealed that the transcript levels of TGFβ1 are induced in the samples treated with methanolic extract of Artemisia absinthium. Furthermore, reduced expression levels of MYC gene was noticed in cancerous cells suggesting antiproliferative properties of the plant. This study further highlights the resistance profile of various microbes by antimicrobial susceptibility test with plant extracts. In addition, antidiabetic effect of Artemisia absinthium have also shown positive results. Our study elucidates the potentials of Artemisia absinthium as a medicinal plant, and highlights the differential expression of genes involved in its mitogenic and anti-proliferative activity with a brief account of its pharmacological action.

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Section: Resultsmentioning

confidence: 90%

Pharmacological activities of Artemisia absinthium and control of hepatic cancer by expression regulation of TGFβ1 and MYC genes

Sohail

Zubaır²,

Hussain³

et al. 2023

PLoS ONE

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“…Y79 cell line was least affected by the extracts even by the most active ANM extract. These observations indicated that the antiproliferative potential with in the same plant depended upon which plant part was extracted and with which solvent [28,29] . Moreover, cancer cell lines were sensitive to different plant extracts to different extents.…”

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confidence: 93%

In vitro Evaluation of Antiproliferative, Lipoxygenase and Xanthine Oxidase Inhibitory Activities of Artemisia nilagirica (C.B.Clarke) Pamp. Leaf Extracts

Bhat¹,

Gul²,

Husain³

et al. 2019

pharmaceutical-sciences

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Bhat et al.: Cytotoxicity and Inhibition of Proinflammatory Enzymes by Artemisia nilagiricaThe current study was performed to screen the extracts of Artemisia nilagirica (C.B. Clarke) Pamp. for antiproliferative and antiinflammatory properties. Antiproliferative activity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Antiinflammatory activity was evaluated in terms of lipoxygenase and xanthine oxidase inhibitory activities. Results indicated that out of the 3 extracts tested, methanol extract was found to inhibit Y79 (human retinoblastoma) and MCF-7 (human breast adenocarcinoma) cell lines. The same extract was also found to inhibit lipoxygenase and xanthine oxidase enzymes in a concentration-dependent manner with an IC 50 of 128.20±3.39 and 144.23±2.04 µg/ ml for lipoxygenase and xanthine oxidase, respectively. The present study highlighted the antiproliferative potential of the plant against cancer cell lines. The extracts inhibited lipoxygenase and xanthine oxidase enzymes. Therefore, this plant could be a valuable source for anticancer and antiinflammatory molecules.

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“…Artemisia absinthium L. (Wormwood), a member of the family Asteraceae, is a perennial herb that is found mainly in the regions of Eurasia, North and South America, and Northern Africa (Bora and Sharma, 2010). This plant is known for its prominent ethnopharmacological significance and is used as a remedy in many diseases due to its antibacterial, antihelminthic, antifungal, antiparasitic, antiseptic, anti-inflammatory, and anticancer activities (Bhat et al, 2018;Mughees et al, 2019). All these activities are ascribed to the presence of different flavonoids, phenolics, and tannins whose type varies with varying geographical origin.…”

Section: Introductionmentioning

confidence: 99%

Pre-clinical toxicity assessment of Artemisia absinthium extract-loaded polymeric nanoparticles associated with their oral administration

et al. 2023

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Background: This study was designed to quantify the composition of the ethanolic extract of Artemisia absinthium through gas chromatography–mass spectrometry analysis and ensure in vivo safety of A. absinthium extract-loaded polymeric nanoparticles (ANPs) before considering their application as a drug carrier via the oral route.Methods: We synthesized N-isopropylacrylamide, N-vinyl pyrrolidone, and acrylic acid crosslinked polymeric NPs by free-radical polymerization reaction and characterized them by Fourier-transform infrared spectroscopy, transmission electron microscopy, and dynamic light scattering spectroscopy. Different concentrations of extract (50 mg/kg, 300 mg/kg, and 2,000 mg/kg body weight) were encapsulated into the hydrophobic core of polymeric micelles for the assessment of acute oral toxicity and their LD50 cut-off value as per the test procedure of OECD guideline 423. Orally administered female Wistar rats were observed for general appearance, behavioral changes, and mortality for the first 30 min, 4 h, 24 h, and then, daily once for 14 days.Result: ANPs at the dose of 300 mg/kg body weight were used as an initial dose, and rats showed few short-lived signs of toxicity, with few histological alterations in the kidney and intestine. Based on these observations, the next set of rats were treated at a lower dose of 50 mg/kg and a higher dose of 2,000 mg/kg ANPs. Rats administered with 50 mg/kg ANPs remained normal throughout the study with insignificant histological disintegration; however, rats treated at 2,000 mg/kg ANPs showed some signs of toxicity followed by mortality among all three rats within 24–36 h, affecting the intestine, liver, and kidney. There were no significant differences in hematological and biochemical parameters among rats treated at 50 mg/kg and 300 mg/kg ANPs.Conclusion: We conclude that the LD50 cut-off value of these ANPs will be 500 mg/kg extract loaded in polymeric NPs.

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Growth Inhibition and Apoptosis Inducing Effects of Artemisia absinthium L. Fractions on Chronic Myeloid Leukemia (K562) Cells

Cited by 3 publications

References 46 publications

Pharmacological activities of Artemisia absinthium and control of hepatic cancer by expression regulation of TGFβ1 and MYC genes

Pharmacological activities of Artemisia absinthium and control of hepatic cancer by expression regulation of TGFβ1 and MYC genes

In vitro Evaluation of Antiproliferative, Lipoxygenase and Xanthine Oxidase Inhibitory Activities of Artemisia nilagirica (C.B.Clarke) Pamp. Leaf Extracts

Pre-clinical toxicity assessment of Artemisia absinthium extract-loaded polymeric nanoparticles associated with their oral administration

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