In vitro Evaluation of Antiproliferative, Lipoxygenase and Xanthine Oxidase Inhibitory Activities of Artemisia nilagirica (C.B.Clarke) Pamp. Leaf Extracts

Abstract

Bhat et al.: Cytotoxicity and Inhibition of Proinflammatory Enzymes by Artemisia nilagiricaThe current study was performed to screen the extracts of Artemisia nilagirica (C.B. Clarke) Pamp. for antiproliferative and antiinflammatory properties. Antiproliferative activity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Antiinflammatory activity was evaluated in terms of lipoxygenase and xanthine oxidase inhibitory activities. Results indicated that out of the 3 extracts tested, methanol extract was found to inhibit Y79 (human retinoblastoma) and MCF-7 (human breast adenocarcinoma) cell lines. The same extract was also found to inhibit lipoxygenase and xanthine oxidase enzymes in a concentration-dependent manner with an IC 50 of 128.20±3.39 and 144.23±2.04 µg/ ml for lipoxygenase and xanthine oxidase, respectively. The present study highlighted the antiproliferative potential of the plant against cancer cell lines. The extracts inhibited lipoxygenase and xanthine oxidase enzymes. Therefore, this plant could be a valuable source for anticancer and antiinflammatory molecules.

Effects of solvent—solvent fractionation on the total terpenoid content and in vitro anti‐inflammatory activity of Serevenia buxifolia bark extract

“…(2017) who stated that the higher the LOX inhibitory assay, the higher the concentration of the Garcinia extract. Plant extracts containing phenolics, flavonoids, and terpenoids have involved to exhibit synergism during the inhibition of LOX activity (Bhat et al., 2019).…”

Lipoxygenase Inhibition by Plant Extracts

“…Bhat et al extracted leaves of Artemisia nilagirica in three different solvents (hexane, methanol, and ethanol) [ 27 ]. All extracted inhibited lipoxygenase, but the most effective was the methanolic extract, with an IC 50 value of 128 µg/mL, followed by hexane extract (196 µg/mL).…”

Anticancer Activity of Silver Nanoparticle of Prodigiosin on Lung Cancer

“…This combination was phenol extracted, and DNA was precipitated in the aqueous phase using 25 µl (1/10 volume) of 7.5 M ammonium acetate and 250 µl (1/1 volume) isopropanol. DNA fragments were seen by exposing the gel to UV light followed by photography after electrophoresis on a 1 percent agarose gel containing 1 µg/ml ethidium bromide at 70 V [21].…”