Growth in Revascularized Bone Grafts in Young Puppies

Donski, P; G, Carwell; Sharzer, Leonard A.

doi:10.1097/00006534-197908000-00017

Cited by 77 publications

(24 citation statements)

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“…Angiogenesis is also critical for successful bone fracture healing [Donski et al, 1979;Steinbrech et al, 2000], but the effects of different kinds of magnetic fields on angiogenesis through vascular growth factors are inconsistent and contradictory. Prior to the studies of SMF, the effects of pulsed electromagnetic fields (PEMF) or electromagnetic fields (EMF) on angiogenesis have been studied in vitro and/or in vivo [Roland et al, 2000;Williams et al, 2001;Tepper et al, 2004].…”

Section: Introductionmentioning

confidence: 99%

Effects of a moderate‐intensity static magnetic field on VEGF‐A stimulated endothelial capillary tubule formation in vitro

Okano

Onmori

Tomita

et al. 2006

Bioelectromagnetics

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Effects of a moderate-intensity static magnetic field (SMF) on the early-stage development of endothelial capillary tubule formation were examined during the initial cell growth periods using co-cultured human umbilical vein endothelial cells and human diploid fibroblasts. The co-cultured cells within a well (16 mm in diameter) were exposed to SMF intensity up to 120 mT (Bmax) with the maximum spatial gradient of 21 mT/mm using a disc-shaped permanent magnet (16 mm in diameter and 2.5 mm in height) for up to 10 days. Control exposure was performed without magnet. Some vascular endothelial cells were treated with vascular endothelial growth factor (VEGF)-A (10 ng/ml) to promote the tubule formation every 2-3 days. Four experimental protocols were performed: (1) non-exposure (control); (2) SMF exposure alone; (3) non-exposure with VEGF-A; (4) SMF exposure with VEGF-A. Photomicrographs of tubule cells immunostained with an anti-platelet-endothelial cell adhesion molecule-1 (PECAM-1 [CD31[) antibody as a pan-endothelial marker, were analyzed after culture at 37 degrees C for 4, 7, and 10 days. The mean values of the area density and the length of tubules (related mainly to arteriogenesis) as well as the number of bifurcations (related mainly to angiogenesis) were determined as parameters of tubule formation and were compared between the groups. After a 10 day incubation, in the peripheral part of the culture wells, SMF alone significantly promoted the tubule formation in terms of the area density and the length of tubules, compared with control group. In the central part of the wells, however, SMF did not cause any significant changes in the parameters of tubule formation. After a 7 day incubation, VEGF-A significantly promoted all the parameters of tubule formation in any part of the wells, compared with control group. With regard to the synergistic effects of SMF and VEGF-A on tubule formation, after a 10 day incubation, SMF significantly promoted the VEGF-A-increased area density and length of tubules in the peripheral part of the wells, compared with the VEGF-A treatment alone. However, SMF did not induce any significant changes in the VEGF-A-increased number of bifurcations in any part of the wells. The tubule cells observed in the wells had elongated, spindle-like shapes, and the direction of cell elongation was random, irrespective of the presence and direction of SMF. These findings suggest that the application of SMF to intact or VEGF-A-stimulated vascular endothelial cells leads mainly to promote or enhance arteriogenesis in the peripheral part of the wells, where the spatial gradient increases relative to the central part. The effects of SMF on the VEGF-A-enhanced tubule formation appear to be synergistic or additive in arteriogenesis but not in angiogenesis.

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Section: Introductionmentioning

confidence: 99%

Effects of a moderate‐intensity static magnetic field on VEGF‐A stimulated endothelial capillary tubule formation in vitro

Okano

Onmori

Tomita

et al. 2006

Bioelectromagnetics

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“…There is good evidence to support the preservation of osteocyte viability after the transfer of non-epiphyseal-containing vascularized bone, [10][11][12][13] but the bone growth may be unpredictable. While experimental evidence supports the hypothesis that vascularized membranous bone grafts transferred to the mandible and zygomaticomaxillary defects in immature animals contribute to normal craniofacial development in a more predictable fashion than nonvascularized bone grafts, 21 there is little evidence to support this clinically.…”

Section: Commentmentioning

confidence: 99%

Reconstruction of the Pediatric Maxilla and Mandible

Genden

Buchbinder²,

Chaplin³

et al. 2000

Arch Otolaryngol Head Neck Surg

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“…We used the vascularized ulna model previously reported (4,12), in which 4 cm of diaphyseal ulna was mobilized through both a dorsal and volar approach. Twenty-four beagle dogs were anesthetized with Nembutal (30 mglkg).…”

Section: Methodsmentioning

confidence: 99%

Experimental vascularized bone grafts: Histopathologic correlations with postoperative bone scan: The risk of false‐positive results

Shaffer

Field

Wilber

et al. 1987

Journal Orthopaedic Research

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A positive bone scan in an experimental model of bilateral ulna diaphyseal bone grafts demonstrated early bone repair in both vascularized and nonvascularized orthotopic ulna autografts. A positive bone scan did not correlate with the perfusion of the vascularized and nonvascularized grafts as measured by microangiograms done 1 week postoperatively. In this model, if the bone scan is intended to monitor the circulatory status and viability of the bone graft, it must be done earlier than 1 week postoperative prior to the onset of creeping repair in both vascularized and nonvascular ulna autografts.

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Growth in Revascularized Bone Grafts in Young Puppies

Cited by 77 publications

References 0 publications

Effects of a moderate‐intensity static magnetic field on VEGF‐A stimulated endothelial capillary tubule formation in vitro

Effects of a moderate‐intensity static magnetic field on VEGF‐A stimulated endothelial capillary tubule formation in vitro

Reconstruction of the Pediatric Maxilla and Mandible

Experimental vascularized bone grafts: Histopathologic correlations with postoperative bone scan: The risk of false‐positive results

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