Growth factors, CD34 positive cells, and fibrin network analysis in concentrated growth factors fraction

Rodella, Luigi Fabrizio; Favero, Gaia; Boninsegna, Ramon; Buffoli, Barbara; Labanca, Mauro; Scarı̀, Giorgio; Sacco, Luigi; Batani, Tiziano

doi:10.1002/jemt.20968

Cited by 224 publications

(246 citation statements)

References 30 publications

(53 reference statements)

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“…One of these products, the concentrated growth factor (CGF), was defined by Sacco in 2006 [35]. CGF also has its own centrifugal technique in a manner similar to PRF.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of effectiveness of concentrated growth factor on osseointegration

et al. 2017

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BackgroundGrowth factor-containing products have been reported to increase implant stability and accelerate osseointegration. Concentrated growth factor (CGF) can be used for this purpose with the growth factors it contains. The aim of this study is to assess the effect of CGF on implant stability and osseointegration.MethodsTwelve patients with maxillary anterior toothless were included in the study. Implant cavities prepared in the study group were covered with CGF membrane before implant placement, but conventional implant placement was performed in the control group. Resonance frequency measurements were performed with the Osstell device intra-operatively, post-operatively, at the 1st week, and at the 4th week.ResultsThe mean ISQ values were found to be 79.40 ± 2.604 for the study group and 73.50 ± 5.226 for the control group at 1st week, 78.60 ± 3.136 for the study group and 73.45 ± 5.680 for the control group at 4th week. The differences between the groups were statistically significant (p < 0.05).ConclusionsIt was observed that the concentrated growth factor had positive effects on implant stabilization. The ISQ measurements at week 1 and week 4 were notably higher in the study group. Application of this material seems to accelerate osseointegration.

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“…One of these products, the concentrated growth factor (CGF), was defined by Sacco in 2006 [35]. CGF also has its own centrifugal technique in a manner similar to PRF.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of effectiveness of concentrated growth factor on osseointegration

et al. 2017

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“…The mechanism responsible for the cell proliferation by CGFs may be explained as follows: CGFs are rich in a variety of growth factors, such as TGF-β, PDGF-AB, VEGF and IGF-Ⅰ (24). These growth factors while functioning on their own (25)(26)(27)(28)(29), are also synergistic and create close contact tissue repair regulatory systems (24,30,31). Unlike PRP, CGFs do not dissolve rapidly following application, instead, the strong fibrin gel in the matrix addition is slowly remodeled in a similar manner to a natural blood clot.…”

Section: Discussionmentioning

confidence: 99%

Effect of concentrated growth factors on beagle periodontal ligament stem cells in vitro

Wang

2013

Molecular Medicine Reports

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Abstract. Identifying a reliable and effective cytokine or growth factor group has been the focus of stem cell osteogenic induction studies. Concentrated growth factors (CGFs) as the novel generation of platelet concentrate products, appear to exhibit a superior clinical and biotechnological application potential, however, there are few studies that have demonstrated this effect. This study investigated the proliferation and differentiation of periodontal ligament stem cells (PDLSCs) co-cultured with CGFs. The rate of proliferation was analyzed by cell counting and an MTT assay. Mineralization nodule counts, alkaline phosphatase activity detection, qPCR, western blot analysis and immunohistochemistry were used to analyze mineralization effects. The results showed that CGF significantly promoted the proliferation of PDLSCs, and exhibited a dose-dependent effect on the activation and differentiation of the stem cells. The application of CGF on PDLSC proliferation and osteoinduction may offer numerous clinical and biotechnological application strategies.

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“…In contrast, the ''de facto standard'' procedure of PRF preparation minimizes the variability incurred by centrifuge speed and operator skill, and enables operators to provide PRF preparations of consistent quality to patients. Therefore, compared to PRP preparations, it is theoretically possible to compare the Choukroun's group and others have theorized and further modified PRF to produce A-PRF (leukocyte-enriched, advanced type), i-PRF (injectable type), and CGF (concentrated growth factors) [48][49][50]. Although the preparation of CGF adopts a seamless two-step centrifuge speed control, CGF is almost identical to PRF.…”

Section: Prfmentioning

confidence: 99%

Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances

Kawase

2015

Odontology

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Over the past decade, platelet-rich plasma (PRP), a platelet-concentrated plasma fraction, has been widely investigated and applied to regenerative medicine. The clinical utility of PRP is supported by evidence that PRP contains high concentrations of platelet-related growth factors and normal concentrations of plasma-derived fibrinogen, both of which contribute synergistically to the regenerative process. Additionally, its superior cost-efficacy versus conventional therapies is attractive to many clinicians. However, current disadvantages of PRP include a relatively complicated preparation procedure and variable operator-dependent efficacy. An additional disadvantage is the use of bovine thrombin, an animal-derived biological, as a coagulant. Many of these disadvantages are overcome by recent advances in preparation procedures and devices; for example, Joseph Choukroun simplified the platelet-rich fibrin preparation procedure and improved handling efficiency without the aid of animal-derived factors. With advancements in cell processing technology, there has been a general shift in cell therapy from autologous to allogeneic treatment; however, autologous PRP therapy will not easily be replaced by allogeneic treatment in the near future. Therefore, to provide more predictable regenerative therapy outcomes using autologous PRP, further investigations should address developing a standardized procedure for PRP preparation to augment its efficacy and potency, independent of donor variability. We would then propose that operators and clinicians prepare PRP according to the standardized protocol and to carefully evaluate the clinical scenario (i.e., recipient factors comprising skeletal defects) to determine which factor(s) should be added to PRP preparations. This careful approach will lead to improved clinical outcomes for patients.

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Growth factors, CD34 positive cells, and fibrin network analysis in concentrated growth factors fraction

Cited by 224 publications

References 30 publications

Evaluation of effectiveness of concentrated growth factor on osseointegration

Evaluation of effectiveness of concentrated growth factor on osseointegration

Effect of concentrated growth factors on beagle periodontal ligament stem cells in vitro

Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances

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