Effect of concentrated growth factors on beagle periodontal ligament stem cells in vitro

Abstract

Abstract. Identifying a reliable and effective cytokine or growth factor group has been the focus of stem cell osteogenic induction studies. Concentrated growth factors (CGFs) as the novel generation of platelet concentrate products, appear to exhibit a superior clinical and biotechnological application potential, however, there are few studies that have demonstrated this effect. This study investigated the proliferation and differentiation of periodontal ligament stem cells (PDLSCs) co-cultured with CGFs. The rate of proliferation was analyzed by cell counting and an MTT assay. Mineralization nodule counts, alkaline phosphatase activity detection, qPCR, western blot analysis and immunohistochemistry were used to analyze mineralization effects. The results showed that CGF significantly promoted the proliferation of PDLSCs, and exhibited a dose-dependent effect on the activation and differentiation of the stem cells. The application of CGF on PDLSC proliferation and osteoinduction may offer numerous clinical and biotechnological application strategies.

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

“…The kinetics of PDGF-AB, VEGF, TNF-α, TGF-β1, IGF-I, BDNF and BMP-2 released from CGF clots were evaluated in triplicate by incubation of the CGF with RPMI 1640 cell medium (Lonza, Verviers, Belgium) without growth supplements for 5 hours, 1, 3, 6, 7 and 8 days (34). The CGF were placed in a 12-well plates (one in each well) with the addition of 1.6 ml of cell culture medium and then incubated at 37°C.…”

“…For example, it has been reported that CGF has a positive effect for the following: sinus and alveolar ridge augmentation [31]; pre-implant augmentation procedures [32]; promotion of in vitro proliferation, osteogenic maturation and mineralization of mesenchymal stem cells and healing of critical-size bone defects in vivo [33]; promotion of in vitro periodontal ligament stem cells proliferation [34], management of chronic venous ulcers [35]. Considering the small amount of data on morphology, biological properties and regenerative potentiality of CGF, the aim of this study was to evaluate the blood cell localization, the in vitro cumulative release of growth factors and the in vitro power of growth on three different types of human cell lines: the Normal Human Dermal Fibroblasts (NHDF), the human umbilical vein endothelial cells (HUVEC) and the Human Osteoblast (HOB).…”

The Effect of Concentrated Growth Factors in the Treatment of Periodontal Intrabony Defects

“…Instead, the strong fibrin gel in the matrix addition is slowly remodeled in a similar manner to a natural blood clot. Thus, CGFs prolong the duration of growth factor activity, which is conducive for growth factor synergy, and enhances cell proliferation and osteogenic differentiation [30]. …”

“…These tubes were then immediately centrifuged (Medifuge, Silfradentsrl, Sofia, Italy) using a program with the following characteristics: 30 s acceleration, 2 min at 2700 rpm, 4 min at 2400 rpm, 4 min at 2700 rpm, 3 min at 3000 rpm and 36 s deceleration and stop. At the end of the process, three blood fractions were created (Figure 1A): a superior phase represented by the serum (blood plasma without fibrinogen and coagulation factors, platelet poor plasma, PPP); an interim phase represented by a very large and dense polymerized fibrin block containing the CGFs, white blood cells and stem cells; and the lower RBC layer [26,30]. …”

Bioactive Platelet Aggregates: Prp, Prgf, Prf, Cgf And Sticky Bone

“…CGF seems to possess a good regenerative capacity and versatility. For example, it has been reported that CGF has a positive effect for the following: sinus and alveolar ridge augmentation [54] ; pre-implant augmentation procedures [55] ; promotion of in vitro proliferation, osteogenic maturation and mineralization of mesenchymal stem cells and healing of critical-size bone defects in vivo [56] ; promotion of in vitro periodontal ligament stem cells proliferation [57] , management of chronic venous ulcers [58] .…”