Group‐specific antigen of murine leukemia viruses in mice of low‐leukemic strains

Абелев, Г. И.; Él'gort, D. A.

doi:10.1002/ijc.2910060202

Cited by 43 publications

(18 citation statements)

References 15 publications

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“…In addition, the stability to storage at -20°C of the material in control sera was less than that of the p30 in leukaemic serum. It is of interest that Abelev and Elgort (1970), using an indirect immunoautoradiographic technique, found trace quantities of group specific internal virion antigens of murine leukaemia viruses in the sera of normal mice of one strain of low leukaemia incidence (i.e. C57B1/6), but not in the serum of Balblc mice, also considered to be a low leukaemia incidence strain.…”

Section: Methodsmentioning

confidence: 99%

Studies on mouse Moloney virus induced tumours: II. Detection of p30 in the serum of mice with Moloney leukaemia by in vitro blocking of complement dependent antibody mediated cytotoxicity

Epstein¹,

Knight²

1975

Br J Cancer

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Sera from Balb/c mice bearing Moloney leukaemia block complement dependent antibody mediated cytotoxicity of an antiserum prepared in rats against syngeneic Moloney virus induced lymphomata when either spleen cells from mice bearing Moloney leukaemia (M) or an in vitro line of Moloney virus transformed cells (MSC) are used as targets. This antiserum has been shown to recognize p30, the major internal virion protein, as a cytotoxic target on these cells. Viral particles were identified by electron microscopic examination of pelleted material obtained from leukaemic sera after high speed centrifugation. However, removal of virus did not affect the capacity of the leukaemic sera to absorb cytotoxicity of rat ILR-3 for MSC targets, and only depressed somewhat its ability to absorb activity of the same antisera against M targets. Virus-free leukaemic sera also blocks complement dependent antibody mediated cytotoxicity of an antiserum prepared in goats against the gs3 determinant of p30. This indicates that the material in leukaemic sera responsible for the in vitro block of antibody mediated cytotoxicity was p30. A lesser degree of block was observed with sera obtained from normal Balb/c mice, but the nature of material responsible is as yet undefined.

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Section: Methodsmentioning

confidence: 99%

Studies on mouse Moloney virus induced tumours: II. Detection of p30 in the serum of mice with Moloney leukaemia by in vitro blocking of complement dependent antibody mediated cytotoxicity

Epstein¹,

Knight²

1975

Br J Cancer

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“…This stable, species-specific antigen is characteristic of C-type viruses regardless of the cellular substrate used to grow the particular virus under study (3)(4)(5). Results of tests for this specific marker have shown widespread occurrence of viral gene activity under conditions where infectious virus or even virus particles could not be detected (6,7). These findings contributed significantly to the theory that viral-genetic information is inherited as a part of the cellular genome (2,8,9).…”

mentioning

confidence: 93%

Group-Specific Antigens of RNA Tumor Viruses as Markers for Subinfectious Expression of the RNA Virus Genome

Gilden

Oroszlan

1972

Proc. Natl. Acad. Sci. U.S.A.

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Antigenic markers associated with the major internal protein of RNA tumor viruses of the C-type have proven extremely useful in natural history studies of these viruses. This protein possesses species-specific antigenic determinants, and, in the case of mammalian Ctype viruses, the protein possesses crossreactive determinants as well. These determinants are, thus, useful for species identification and classification of mammalian viruses. A unique distribution of antigens in embryonic tissues of several species (where tests are available) was detected, and in addition, antigen expression in tissues appears to be controlled by a dominant gene. These data have contributed greatly to the theory that RNA tumorvirus information is inherited as part of the cellular genome.

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“…Type-C viral protein antigens have been detected in tissues of a large number of different strains of mice, including low leukemia strains (12)(13)(14)(15)(16). For most of these early studies, the specific proteins measured in assays of type-C virus group specific (gs) antigens is unknown.…”

mentioning

confidence: 99%

Host Control of Endogenous Murine Leukemia Virus Gene Expression: Concentrations of Viral Proteins in High and Low Leukemia Mouse Strains

Strand¹,

Lilly²,

August³

1974

Proc. Natl. Acad. Sci. U.S.A.

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Two of the major molecular components of murine leukemia virus particles, the internal protein (p30) and the envelope glycoprotein (gp69/71) have been measured in the spleens of normal, 6-to 10-week-old mice of various inbred strains and crosses. Both proteins were detected in virtually all mice. Extracts of high leukemia, high murine leukemia virus strains (AKR, C58, PL) showed high levels of both proteins; extracts of other strains usually showed lower levels. Of particular interest, however, were the exceptions to these general observations: (1) Very little gp69/71 could be detected in spleens of BALB mice, and this trait was dominant in crosses with AKR and DBA/2, both of which express a high level of gp69/71. Thymus-deficient BALB/c-nu/nu (nude) mice, in contrast, showed a higher concentration of gp69/71 typical of other low leukemia strains, suggesting that the virtual absence of the protein in normal BALB/c mice may result from immunologic suppression. (2) With C57L, C57BL/l0Sn, and C57BL/6 strains the concentration of p30 was lower, in some cases much lower, than would be expected from the concentration of gp69/71. (3) DBA/2 mice showed high levels of gp69/71, 10-fold greater than that of p30, whereas congenic DBA/2-Fv-lb mice showed the opposite pattern. (4) Mice of 129-Gix-strain showed no detectable levels of either p30 or gp69/71 proteins, although the congenic 129 (Gix+) showed appreciable levels of both. The absence of these proteins in 129-Gix-mice is a recessive trait, as F1 hybrids with AKR and DBA/2 showed appropriate levels of both proteins. It is concluded that expression of viral p30 and gp69/71 proteins in mice is not coordinately linked and that expression is complex, being influenced by several genes, including Gv-1, H-2, Fv-I, and probably still others.Evidence from many sources, culminating with the demonstration that clonal lines of virus-free mouse embryo cells can be induced to yield type-C RNA viruses, has suggested that the genetic determinants of murine leukemia viruses (Mu-LV) are present as heritable components in all mouse cells (1)(2)(3)(4)(5).This viral gene information is under several forms of host control and may exist in unexpressed form for many generations. One extensively studied genetic factor is the host range restriction system governed by the Fv-1 gene (6). In this system, N-tropic MuLV readily propagates in N-type (Fve-i homozygous) cells but grows poorly in B-type (Fv-1b homozygous) cells; B-tropic viruses show the reciprocal pattern, and both types of virus grow poorly in heterozygous cells (7,8). A second major genetic system has been elucidated in studies of the inheritance of infectious MuLV or of MuLV proteins in crosses of AKR, a high leukemia strain with high levels of infectious MuLV and viral proteins, with strains showing little or no MuLV or viral protein (9-11). These studies showed that two unlinked, dominant chromosomal loci, Akv-1 and Akv-2, are the determinants of MuLV in AKR mice, and both loci have the capacity of eliciting virus expres...

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Group‐specific antigen of murine leukemia viruses in mice of low‐leukemic strains

Cited by 43 publications

References 15 publications

Studies on mouse Moloney virus induced tumours: II. Detection of p30 in the serum of mice with Moloney leukaemia by in vitro blocking of complement dependent antibody mediated cytotoxicity

Studies on mouse Moloney virus induced tumours: II. Detection of p30 in the serum of mice with Moloney leukaemia by in vitro blocking of complement dependent antibody mediated cytotoxicity

Group-Specific Antigens of RNA Tumor Viruses as Markers for Subinfectious Expression of the RNA Virus Genome

Host Control of Endogenous Murine Leukemia Virus Gene Expression: Concentrations of Viral Proteins in High and Low Leukemia Mouse Strains

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