Green tea extract activates AMPK and ameliorates white adipose tissue metabolic dysfunction induced by obesity

Rocha, Andréa L.; Bolin, Anaysa Paola; Cardoso, Cláudia Andréa Lima; Otton, Rosemari

doi:10.1007/s00394-015-1033-8

Cited by 79 publications

(101 citation statements)

References 27 publications

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“…Moreover, EGCG prevents fatty liver by AMPK activation via liver kinase B1 inhibiting mediators responsible for the synthesis of fatty acid and de novo lipogenesis in mice fed a HFD [38]. In addition, similar effects of GT supplementation in the prevention of the deleterious effects of HFD have been supported by Rocha et al, whose results suggested that GT extract could improve WAT metabolic dysfunction induced via activation of AMPK [21]. Thus, GT supplementation contributed to the energy homeostasis of adipose tissue via the regulation of the AMPK signaling pathway in DIO mice.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Green Tea Extract on Systemic Metabolic Homeostasis in Diet-Induced Obese Mice Determined via RNA-Seq Transcriptome Profiles

et al. 2016

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Green tea (GT) has various health effects, including anti-obesity properties. However, the multiple molecular mechanisms of the effects have not been fully determined. The aim of this study was to elucidate the anti-obesity effects of GT via the analysis of its metabolic and transcriptional responses based on RNA-seq profiles. C57BL/6J mice were fed a normal, high-fat (60% energy as fat), or high-fat + 0.25% (w/w) GT diet for 12 weeks. The GT extract ameliorated obesity, hepatic steatosis, dyslipidemia, and insulin resistance in diet-induced obesity (DIO) mice. GT supplementation resulted in body weight gain reduction than mice fed high-fat through enhanced energy expenditure, and reduced adiposity. The transcriptome profiles of epididymal white adipose tissue (eWAT) suggested that GT augments transcriptional responses to the degradation of branched chain amino acids (BCAAs), as well as AMP-activated protein kinase (AMPK) signaling, which suggests enhanced energy homeostasis. Our findings provide some significant insights into the effects of GT for the prevention of obesity and its comorbidities. We demonstrated that the GT extract contributed to the regulation of systemic metabolic homeostasis via transcriptional responses to not only lipid and glucose metabolism, but also amino acid metabolism via BCAA degradation in the adipose tissue of DIO mice.

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Section: Discussionmentioning

confidence: 99%

“…In particular, GT has been shown to increase energy expenditure [18] and enhance the metabolic rate and fat-burning ability [19,20]. Most recently, Rocha and colleagues [21] reported that GT extract activates AMP-activated protein kinase (AMPK) and ameliorates WAT metabolic dysfunction that is induced by obesity.…”

Section: Introductionmentioning

confidence: 99%

Effect of Green Tea Extract on Systemic Metabolic Homeostasis in Diet-Induced Obese Mice Determined via RNA-Seq Transcriptome Profiles

et al. 2016

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“…Insulin resistance is considered to be responsible for the development of all constituents of the metabolic syndrome, which in turn may have cardiovascular and metabolic consequences [39]. Green tea, capsaicin and ginger intake may improve markers of insulin metabolism through increased AMP-activated protein kinase activation, modulating the expression of genes involved in metabolism, particularly in adipose tissue [40] and decreasing apoptosis of β-cells by potentiating insulin/IGF-1 signaling [35]. The discrepancies between findings of this study and those of previous reports might be explained by doses of green tea, capsaicin and ginger supplements used, the intervention time, clinical characteristics of the participants such as variation in baseline levels of metabolic profiles.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Dietary Supplements Containing Green Tea, Capsaicin and Ginger Extracts on Weight Loss and Metabolic Profiles in Overweight Women: A Randomized Double-Blind Placebo-Controlled Clinical Trial

Taghizadeh

Farzin²,

Taheri³

et al. 2017

Ann Nutr Metab

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Background: This study was conducted to determine the effects of dietary supplements containing green tea, capsaicin and ginger extracts on weight loss and metabolic profiles among overweight women. Methods: This randomized double-blind placebo-controlled clinical trial was implemented among 50 overweight women. Participants were randomly divided into 2 groups. Group A received dietary supplements containing 125 mg green tea, 25 mg capsaicin and 50 mg ginger extracts (n = 25) group B received placebos (n = 25) twice with lunch and twice with dinner daily for 8 weeks. Results: Compared with placebo, taking dietary supplements containing green tea, capsaicin and ginger resulted in a significant decrease in weight (-1.8 ± 1.5 vs. +0.4 ± 1.2 kg, respectively, p < 0.001) and body mass index (BMI; -0.7 ± 0.5 vs. +0.1 ± 0.5 kg/m², respectively, p < 0.001). In addition, subjects who received green tea, capsaicin and ginger co-supplements had significantly decreased serum insulin concentrations (-2.6 ± 3.9 vs. -0.6 ± 2.0 µIU/mL, p = 0.02), homeostatic model of assessment for insulin resistance (-0.5 ± 0.8 vs. -0.05 ± 0.6, p = 0.01), and increased quantitative insulin sensitivity check index (+0.01 ± 0.01 vs. +0.001 ± 0.01, p = 0.008) and plasma glutathione (GSH) levels (+73.8 ± 120.6 vs. -28.3 ± 193.4 µmol/L, p = 0.03) compared with the placebo. Conclusions: Our study indicated that taking green tea, capsaicin and ginger co-supplements for 8 weeks among overweight women had beneficial effects on weight, BMI, markers of insulin metabolism and plasma GSH levels.

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“…The potential therapeutic benefits of V . angustifolium and naturally occurring phenolic compounds are in part due to regulated secretion of extracellular matrix degrading enzymes and/or their inhibitors through adenosine monophosphate‐activated protein kinase (AMPK) cell signalling (Banskota, Regmi, & Kim, ; Hwang et al, ; Ong, Hsu, & Tan, ; Rocha, Bolin, Cardoso, & Otton, ; Tong, Wang, Sun, & Suo, ; Vuong, Martineau, Ramassamy, Matar, & Haddad, ). Although the effects of V .…”

Section: Introductionmentioning

confidence: 99%

Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro

Ferrier

Gaudet

et al. 2018

Phytotherapy Research

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Perturbations to extravillous trophoblast (EVT) cell migration and invasion are associated with the development of placenta-mediated diseases. Phytochemicals found in the lowbush blueberry plant (Vaccinium angustifolium) have been shown to influence cell migration and invasion in models of tumorigenesis and noncancerous, healthy cells, however never in EVT cells. We hypothesized that the phenolic compounds present in V. angustifolium leaf extract promote trophoblast migration and invasion. Using the HTR-8/SVneo human EVT cell line and Boyden chamber assays, the influence of V. angustifolium leaf extract (0 to 2 × 10 ng/ml) on trophoblast cell migration (n = 4) and invasion (n = 4) was determined. Cellular proliferation and viability were assessed using immunoreactivity to Ki67 (n = 3) and trypan blue exclusion assays (n = 3), respectively. At 20 ng/ml, V. angustifolium leaf extract increased HTR-8/SVneo cell migration and invasion (p < .01) and did not affect cell proliferation or viability. Chlorogenic acid was identified as a major phenolic compound of the leaf extract and the most active compound. Evidence from Western blot analysis (n = 3) suggests that the effects of the leaf extract and chlorogenic acid on trophoblast migration and invasion are mediated through an adenosine monophosphate-activated protein (AMP) kinase-dependent mechanism. Further investigations examining the potential therapeutic applications of this natural health product extract and its major chemical compounds in the context of placenta-mediated diseases are warranted.

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Green tea extract activates AMPK and ameliorates white adipose tissue metabolic dysfunction induced by obesity

Abstract: Our study identified that metabolic changes caused by GT intake induced AMPK activation and modulate the expression of genes involved in metabolism, particularly in adipose tissue, thus offering a therapeutic strategy to combat insulin resistance, dyslipidemia, and obesity in rats.

Cited by 79 publications

References 27 publications

Effect of Green Tea Extract on Systemic Metabolic Homeostasis in Diet-Induced Obese Mice Determined via RNA-Seq Transcriptome Profiles

Effect of Green Tea Extract on Systemic Metabolic Homeostasis in Diet-Induced Obese Mice Determined via RNA-Seq Transcriptome Profiles

The Effect of Dietary Supplements Containing Green Tea, Capsaicin and Ginger Extracts on Weight Loss and Metabolic Profiles in Overweight Women: A Randomized Double-Blind Placebo-Controlled Clinical Trial

Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro

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