Green-Synthesized Silver Nanoparticles Induced Apoptotic Cell Death in MCF-7 Breast Cancer Cells by Generating Reactive Oxygen Species and Activating Caspase 3 and 9 Enzyme Activities

Ullah, Ikram; Khalil, Ali Talha; Ali, Muhammad; Iqbal, Javed; Ali, Waqar; Alarifi, Saud; Shinwari, Zabta Khan

doi:10.1155/2020/1215395

Cited by 108 publications

(65 citation statements)

References 84 publications

(79 reference statements)

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“…AgNPs generate different biochemical pathways, including ROS-mediated mitochondrial dysfunction, DNA damage, and apoptosis, that are related to the enhanced anticancer activities in MCF-7 cells. 53 , 59 The results we obtained in this study are in line with the findings of researchers in previous examinations. Mugade et al bioengineered (mannan sulfate-capped AgNPs) to downregulate TNF-α and IL-6 expression in rats.…”

Section: Discussionsupporting

confidence: 93%

Silver Nanoparticle-Coated Ethyl Cellulose Inhibits Tumor Necrosis Factor-α of Breast Cancer Cells

Abdellatif

Alsharidah

Rugaie

et al. 2021

DDDT

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Introduction: Cancer is one of the leading causes of death worldwide. In many cases, cancer is related to the elevated expression of a significant cytokine known as tumor necrosis factor-α (TNF-α). Breast cancer in particular is linked to increased proliferation of tumor cells, high incidence of malignancies, more metastases, and generally poor prognosis for the patient. The research sought to assess the effect of silver nanoparticles reduced with ethyl cellulose polymer (AgNPs-EC) on TNF-α expression in MCF-7 human breast cancer cells. Methods: The AgNPs-EC were produced using the green synthesis reduction method, and their formation was proofed via UV-VIS spectroscopy. Furthermore, AgNPs-EC were characterized for their size, charge, morphology, Ag ion release, and stability. The MCF-7 cells were treated with AgNPs-EC. Then, the expression of TNF-α genes was determined through PCR in real time, and protein expression was studied using ELISA. Results: The AgNPs-EC were spherical with an average size of 150±5.1 nm and a zetapotential of −41.4±0.98 mV. AgNPs-EC had an inhibitory effect on cytokine mRNA and protein expression levels, which suggests that they could be used safely in the fight against cancer. AgNPs-EC cytotoxicity was also found to be non-toxic to MCF-7. Conclusion:Our data determined AgNPs-EC as a novel inhibitor of TNF-α production. These results are promising for developing novel therapeutic approaches for the future treatment of cancer with safe materials.

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Section: Discussionsupporting

confidence: 93%

Silver Nanoparticle-Coated Ethyl Cellulose Inhibits Tumor Necrosis Factor-α of Breast Cancer Cells

Abdellatif

Alsharidah

Rugaie

et al. 2021

DDDT

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“…However, the mechanism through which ZnO-NP is capable of conferring partly differential responses remains controversial. A majority of researchers believe that differential physicochemical characteristics of nanoparticles relative to the bulk contributed to differences in their uptake kinetics, subsequent interactions with biomolecules, signaling, and responses [1][2][3][10][11][12]22,27,28]. More studies are, therefore, required to confirm this hypothesis.…”

Section: Plos Onementioning

confidence: 99%

“…Nowadays, nanotechnology provides great opportunities for development in a plethora of disciplines, including biotechnology and agriculture [1][2][3]. Taking account of agriculture's requirements, attempts have been made to enhance crop productivity and fruit quality, to improve immunity, and to reduce the consumption of chemical fertilizers/pesticides [4,5].…”

Section: Introductionmentioning

confidence: 99%

Zinc oxide nanoparticles mediated substantial physiological and molecular changes in tomato

et al. 2021

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There has long been debate about how nanoproducts meet agricultural requirements. This study aimed to investigate tomato responses to the long-time foliar application of zinc oxide nanoparticles (ZnO-NP; 0 and 3 mgl-1) or bulk type (BZnO). Both ZnO-NP and BZnO treatments, especially the nanoform, were significantly capable of improving growth, biomass, and yield. The ZnO-NP treatment upregulated the expression of the R2R3MYB transcription factor by 2.6 folds. The BZnO and ZnO-NP treatments transcriptionally up-regulated WRKY1 gene by 2.5 and 6.4 folds, respectively. The bHLH gene was also upregulated in response to BZnO (2.3-fold) or ZnO-NP (4.7-fold). Moreover, the ZnO-NP application made a contribution to upregulation in the EREB gene whereas the bulk compound did not make a significant change. Upregulation in the HsfA1a gene also resulted from the ZnO-NP (2.8-fold) or BZnO (1.6-fold) supplementation. The MKK2 and CAT genes displayed a similar upregulation trend in response to the supplements by an average of 3-folds. While the application of ZnO-NP slightly down-regulated the histone deacetylases (HDA3) gene by 1.9-fold, indicating epigenetic modification. The supplements, especially the nano-product, enhanced concentrations of K, Fe, and Zn in both leaves and fruits. The concentrations of Chla, Chlb, and carotenoids were increased in response to the BZnO or ZnO-NP treatments. Likewise, BZnO or ZnO-NP mediated an increase in activity of nitrate reductase and proline content in leaves. These treatments increased soluble phenols and phenylalanine ammonia-lyase activity. With a similar trend, the BZnO or ZnO-NP application improved the activities of catalase and peroxidase enzymes. The reinforcement in metaxylem and secondary tissues resulted from the applied supplements. This study provides comprehensive comparative evidence on how ZnO-NPs may remodel the chromatin ultrastructure and transcription program, and confer stress tolerance in crops. This study also underlines the necessity of providing integrated transcriptome and proteome data in future studies.

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“…Reactive oxygen species (ROS) produced in normal cells and have roles in many physiological conditions [21]. Under excessiveness of ROS, a series of cellular events were occurring results in lipid peroxidation, DNA damaging, cell cycle arrest, and promote apoptosis pathway [22]. It has been reported that new strategies for targeting ROS contribute to the cancer therapy [23].…”

Section: Measurement Of Reactive Oxygen Species Productionmentioning

confidence: 99%

“…In the current study, we evaluated the roles of pro-apoptotic Bax and CAD regulation in relation to the apoptotic death of cancer cells [22]. The mRNA expression levels of genes in AgNPs-OAL -mediated apoptotic cells were displayed by qRT-PCR method, which is presented in Fig.…”

Section: Agnps-oal Induce Apoptosis In Mda-mb231 Cellsmentioning

confidence: 99%

Antineoplastic Efectiveness of Silver Nanoparticles Synthesized from Onopordum Acanthium L. extract (AgNPs-OAL) toward MDA-MB231 Breast Cancer Cells

Delalat

Shandiz

Pakpour

2021

Preprint

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The present research was done to investigate the anticancer properties of silver nanoparticles (AgNPs) fabricated using bioactive extract of Onopordum acanthium L. (AgNPs-OAL) against breast cancer cell MDA_MB231 in vitro. The determination studies of AgNPs-OAL were confirmed by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM) analysis. Interestingly, FESEM image observed the spherical shape of AgNPs-OAL with the range of 1–100 nm. As AgNP-OAL exhibited significant cytotoxicity properties on breast cancer MDA_MB231 cells with IC50 values of 66.04 μg/mL, while lowing toxicity toward normal human embryonic kidney 293 (HEK293) cells with IC50 values of 101.04 μg/mL was evaluated. Further, up-regulation of apoptotic Bax and CAD genes expressions were confirmed by quantitative real-time reverse transcription-PCR (qRT-PCR) technique results. Moreover, enhanced cell cycle population (sub-G1), annexin V/PI staining, acridine orange and ethidium bromide (AO/EB) staining, Hoescht 33258 dye, and generation of reactive oxygen species (ROS) observed in AgNP-OAL-treated MDA_MB231 cancer cells. The green-synthesized AgNP-OAL has promising anticancer efficiency that can trigger apoptosis pathway in the MDA_MB231 breast cancer cells.

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Green-Synthesized Silver Nanoparticles Induced Apoptotic Cell Death in MCF-7 Breast Cancer Cells by Generating Reactive Oxygen Species and Activating Caspase 3 and 9 Enzyme Activities

Cited by 108 publications

References 84 publications

Silver Nanoparticle-Coated Ethyl Cellulose Inhibits Tumor Necrosis Factor-α of Breast Cancer Cells

Silver Nanoparticle-Coated Ethyl Cellulose Inhibits Tumor Necrosis Factor-α of Breast Cancer Cells

Zinc oxide nanoparticles mediated substantial physiological and molecular changes in tomato

Antineoplastic Efectiveness of Silver Nanoparticles Synthesized from Onopordum Acanthium L. extract (AgNPs-OAL) toward MDA-MB231 Breast Cancer Cells

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