The current research was carried out to reveal the possible impacts of cold plasma on growth and physiology of wheat, as a new approach in plant science. Short and long-term impacts of different types of plasma (nitrogen and helium) with surface power density of 0.4 W cm-2 , exposure times (15, 30, 60, and 120 s), and repetitions (1, 2, and 4 times with 24 h intervals) were evaluated. Single-time applied helium or nitrogen derived plasma significantly promoted total root and shoot lengths, in contrast to four times application, and the root system was more sensitive than the shoot one. In addition, seedlings were more sensitive to nitrogen derived plasma, compared with helium. The physiological responses to plasma treatment were analyzed via protein assay and peroxidase or phenylalanine ammonia lyase (PAL) activities measurements. Plasma generated signaling molecules, especially ozone, nitric oxide, and/or UV radiation induced promotions in the peroxidase and PAL activities as well as increase in protein content in leaves, especially when times and/or repetitions increased. Plants were perished by the nitrogen derived plasma at the highest exposure time and number of repetitions. However, the seedlings with inhibited growth not only caught up control one month after, but even the growth rate and biomass accumulation in the shoot and leaves were accelerated. Increased leaf soluble phenol content was recorded in plasma treated seedlings, especially at longer times and more repetitions.
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