Silver Nanoparticle-Coated Ethyl Cellulose Inhibits Tumor Necrosis Factor-α of Breast Cancer Cells

Abstract

Introduction: Cancer is one of the leading causes of death worldwide. In many cases, cancer is related to the elevated expression of a significant cytokine known as tumor necrosis factor-α (TNF-α). Breast cancer in particular is linked to increased proliferation of tumor cells, high incidence of malignancies, more metastases, and generally poor prognosis for the patient. The research sought to assess the effect of silver nanoparticles reduced with ethyl cellulose polymer (AgNPs-EC) on TNF-α expression in MCF-7 human breast cancer cells. Methods: The AgNPs-EC were produced using the green synthesis reduction method, and their formation was proofed via UV-VIS spectroscopy. Furthermore, AgNPs-EC were characterized for their size, charge, morphology, Ag ion release, and stability. The MCF-7 cells were treated with AgNPs-EC. Then, the expression of TNF-α genes was determined through PCR in real time, and protein expression was studied using ELISA. Results: The AgNPs-EC were spherical with an average size of 150±5.1 nm and a zetapotential of −41.4±0.98 mV. AgNPs-EC had an inhibitory effect on cytokine mRNA and protein expression levels, which suggests that they could be used safely in the fight against cancer. AgNPs-EC cytotoxicity was also found to be non-toxic to MCF-7. Conclusion:Our data determined AgNPs-EC as a novel inhibitor of TNF-α production. These results are promising for developing novel therapeutic approaches for the future treatment of cancer with safe materials.

Characterization and Anticancer Activity of Biosynthesized Au/Cellulose Nanocomposite from Chlorella vulgaris

“…The anticancer action of the nanocomposite can be connected to the induction of apoptosis in MCF-7 cells via blocking the activation and nuclear translocation of TNF-mRNA and protein expression. TNF-induced inflammation and toxicity are both exacerbated by AgNPs-EC [80,81].…”

[Retracted] Silver Nanoparticles Stabilized by Poly (Vinyl Pyrrolidone) with Potential Anticancer Activity towards Prostate Cancer

“…e era of nanomedicine and nanoparticulate drug delivery systems has begun long ago and shown significant success in different fields, especially tumor diagnosis and treatment [4][5][6][7][8][9]. Nanoparticles directed at cancer have been shown to be promising materials in this area.…”

Retardation of Bacterial Biofilm Formation by Coating Urinary Catheters with Metal Nanoparticle-Stabilized Polymers

“…Garcia et al [ 59 ] also reported a similar result. Regarding AgNPs-EC, it was reported in our research group that the negatively charged EC facilitates the attraction of Ag+ to the EC chains, followed by reduction with the present, reducing groups in the polymer chain [ 49 ]. In addition, the observed surface plasmon resonance of the produced NPs shifted to a higher wavelength than the AgNO 3 value (the reported λ max , 390 nm), following other researchers [ 59 ].…”

“…Thus, the use of surface stabilizing agents for AgNPs dispersions is mandatory. Polyvinyl pyrrolidone (PVP), polyethylene glycol (PEG), and ethyl cellulose (EC) are amongst the most popular stabilizing agents for AgNPs dispersion due to their good biocompatibility and low cytotoxicity [ 47 , 48 , 49 , 50 ]. In addition, as previously mentioned, AgNPs stabilized with PVP and EC showed proven antibacterial properties, especially with highly resistant strains of E. coli .…”