Green quantitative spectrofluorometric analysis of rupatadine and montelukast at nanogram scale using direct and synchronous techniques

Abstract

Two green-sensitive spectrofluorometric methods were investigated for assay of rupatadine (RUP) [method I] and its binary mixture with montelukast (MKT) [method II]. Method I depends on measuring native fluorescence of RUP in the presence of 0.10 M H 2 SO 4 and 0.10%w/v sodium dodecyl sulfate at 455 nm after excitation at 277 nm. The range of the first method was 0.20–2.00 µg ml −1 with detection and quantitation limits of 59.00 and 179.00 ng ml −1 , respectively. Method II depends on the first derivative synchronous spectrofluorometry. The derivative intensities were measured for the two drugs in an aqueous solution containing Mcllvaine's buffer pH 2.60 at fixed Δ λ of 140 nm. Each drug was estimated at zero-contribution of the other. The intensity was measured at 261 and 371 nm for RUP and MKT, respectively. The method was linear over 0.10–4.00 and 0.20–1.60 µg ml −1 with limits of detection 31.00 and 66.00 ng ml −1 and limits of quantitation 94.00 and 200.00 ng ml −1 for RUP and MKT, respectively. The method was extended to determine this mixture in laboratory-prepared mixtures and combined tablets. Method validation was performed according to ICH guidelines. Statistical interpretation of data revealed good agreement with the comparison method. Method greenness was confirmed by applying three different assessment tools.

The first spectrofluorimetric protocol for sensitive quantitative analysis of bromocriptine in its pure and pharmaceutical forms: evaluation of the greenness of the method

“…As a result of binding the drug to the SDS micellar system, restriction of intramolecular free rotation and increased rigidity of the drug molecules will occur, potentially increasing the drug fluorescence quantum yield. 18–20…”

A comprehensive review of the analytical methods used for the determination of selected antihistamines in pharmaceutical dosage forms and biological fluids

“…The native fluorescence of RUP was examined at 455 nm after excitation at 277 nm and synchronized with MKT determined via first order derivative technique (Ghonim, El-Awady, Tolba, & Ibrahim, 2021). RUP was derivatized using erythrosine B via Resonance Rayleigh scattering and spectrofluorometric approaches (Almahri, Abdel-Lateef, Samir, Derayea, & El Hamd, 2021).…”

Sustainable PVP-Capped Silver Nanoparticles as a Free-Standing Nanozyme Sensor for Visual and Spectrophotometric Detection of Hg2+ in Water Samples: A Green Analytical Method

“…In quantitative analysis, the "greenness" of a proposed analytical method is seen as a difficulty because, in emergency situations, organic dangers are occasionally utilized in large quantities and/or with tired instruments. Optimizing the experimental needs of these organic hazards and the utilized instruments indicated the greenness of such methods [78,79]. Our objective in the developed study was to guard environmental and human health, in line with the general meaning of the twelve principles of green analytical chemistry [80].…”