Two green-sensitive spectrofluorometric methods were investigated for assay of rupatadine (RUP) [method I] and its binary mixture with montelukast (MKT) [method II]. Method I depends on measuring native fluorescence of RUP in the presence of 0.10 M H
2
SO
4
and 0.10%w/v sodium dodecyl sulfate at 455 nm after excitation at 277 nm. The range of the first method was 0.20–2.00 µg ml
−1
with detection and quantitation limits of 59.00 and 179.00 ng ml
−1
, respectively. Method II depends on the first derivative synchronous spectrofluorometry. The derivative intensities were measured for the two drugs in an aqueous solution containing Mcllvaine's buffer pH 2.60 at fixed Δ
λ
of 140 nm. Each drug was estimated at zero-contribution of the other. The intensity was measured at 261 and 371 nm for RUP and MKT, respectively. The method was linear over 0.10–4.00 and 0.20–1.60 µg ml
−1
with limits of detection 31.00 and 66.00 ng ml
−1
and limits of quantitation 94.00 and 200.00 ng ml
−1
for RUP and MKT, respectively. The method was extended to determine this mixture in laboratory-prepared mixtures and combined tablets. Method validation was performed according to ICH guidelines. Statistical interpretation of data revealed good agreement with the comparison method. Method greenness was confirmed by applying three different assessment tools.
The non-steroidal anti-inflammatory medication acemetacin was assessed via two straightforward green spectrofluorimetric techniques. The quenching-dependent derivatizing spectrofluorimetric reactions are the master point of this study. Acriflavine-based method (Method I) depends on forming an ion association complex between acriflavine and the drug in a ratio of 1:1, decreasing the former's fluorescence intensity. Acriflavine or Ag NP's intensity-related quenching action goes linearly with the acemetacin concentration in the 2.0–20.0 µg/mL and 1.0–16.0 µg/mL ranges, respectively. The second quenching mechanism depends on using the silver nanoparticles (Ag NP's) as a fluorescence probe (Method II); Ag NP's were prepared from reducing silver nitrate using sodium borohydride. Both methods could be applied to determine pure and pharmaceutical dosage forms of acemetacin. The methods proved valid according to the international conference on harmonization (ICH) guidelines. In addition to this, this work has been estimated under green criteria assessment tools. There is no significant difference between the proposed and the comparison methods after the statistical interpretation.
Antihistaminic drugs are a group of therapeutic agents representing one of the most-widely prescribed medications for treating different diseases. They are often used to relieve symptoms of allergies, such as hay fever, conjunctivitis, and reactions to insect bites or stings. A comprehensive review with more than two hundred references reported on the analysis of selected antihistamines is explored. The selected drugs belong to different generations of antihistaminic drugs. These drugs include rupatadine, montelukast, desloratadine and fexofenadine.. The literature review covers most methods described for determining these drugs in bulk powder, pharmaceutical dosage forms, animal tissues and biological fluids. Different analytical techniques include UV and Visible spectrophotometry, spectrofluorometry, thin-layer liquid chromatography, high-pressure liquid chromatography, ultra-performance liquid chromatography, gas chromatography, capillary electrophoresis, and electrochemical methods.
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