Green and Efficient Extraction of Polysaccharides From Poria cocos F.A. Wolf by Deep Eutectic Solvent

Abstract

Deep eutectic solvents (DESs) were proposed for the extraction of polysaccharides from Poria cocos (PCPs). Six types of DESs were prepared, and the DES composed of choline chloride and oxalic acid was proved to be suitable. Based on the results of single-factor test, the Box-Behnken experimental design with response surface methodology was carried out, giving the optimal extraction conditions including mole ratio of 1:2 (choline chloride:oxalic acid) and extraction 15 minutes at 100°C. Under the optimal extraction conditions, the extraction yield (46.24% ± 0.13%) was 8.6 times higher than that of hot water. The reusability of DES was demonstrated by a 6-run test, and an extraction yield of PCP was 38.40% ± 0.23% after reusing for 6 times without adding any additional chemicals. Moreover, molecular weight distributions of the resulting PCP were analyzed, and then mainly distributed in the range of 753 to 3578 g/mol. Therefore, DESs were proved to be an excellent extraction solvent alternative to the extraction of PCP.

Ultrasound-Assisted Deep Eutectic Solvents Extraction of Polysaccharides From Morchella importuna: Optimization, Physicochemical Properties, and Bioactivities

“…Effects of DES-5 and DES-6 on the extraction yields of MIPs were significantly lower than those obtained by hot water. DES-1 exhibited higher extraction efficiency than others, which might be attributed to its stronger electrostatic interaction between DES-1 and MIPs than those of other DESs ( 20 ). Furthermore, oxalic acid and choline chloride are non-toxic substances and widely found in natural foods ( 21 ).…”

“…Study indicated that the liquid–solid ratio could affect the extraction efficiency of polysaccharides ( 20 ). As shown in Figure 1F , the effect of liquid–solid ratios on the extraction efficiency of MIPs was measured in this experiment.…”

Wen Dan Tang: A Potential Jing Fang Decoction for Headache Disorders?

“…Traditionally, Fu Ling has been used for medicinal purposes for more than a thousand years [ 214 ]. The pharmacological actions of PCPs in Fu Ling include antibacterial, antitumor, anti-hyperglycemic, immunomodulatory, anti-inflammatory, immunostimulatory, anti-oxidative, anti-aging, anti-hepatic, anti-diabetic, and anti-hemorrhagic fever effects [ 200 , 213 , 215 , 216 ]. Additionally, Sun et al showed in their study that PCPs improved hyperglycemia, hyperlipidemia, and hepatic steatosis in mice [ 217 ].…”

Seaweed-based polysaccharides – review of extraction, characterization, and bioplastic application

“…Seven type III DESs, combined with SWE at 150 °C, 20 bar pressure, 70% water content, and a 1 : 36 S : L ratio, yielded high alginate (28% DW) and fucoidan (15% DW) from defatted Saccharina japonica . 86 Similarly, a DES (choline chloride : 1,4-butanediol; 1 : 5) with MWE at 168 °C and 32% water content produced potent fucoidan extracts from Fucus vesiculosus , exhibiting strong antioxidant and antiproliferative effects. 87 A DES consisting of choline chloride and 1.2-propanediol (1 : 2) with a 30% water content, a 1 : 30 S : L ratio, and ultrasonication (30 min, 40% amplitude) at 70 °C rapidly extracted polysaccharides with an 11% yield from Sargassum horneri .…”